AB273530

Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag)

Name: Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530) | Abcam
Brand: Abcam Limited
SKU: AB273530

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(7 Publications)

Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) is a SARS-CoV-2 Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >95%, suitable for ELISA, WB, SDS-PAGE.

View Alternative Names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

14 Images

ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

ELISA

Lab

ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Plates were coated with Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, ab273530) and Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 500 ng/ml.

Primary antibody (ab271180) concentration range 0.045-100 ng /ml.

Pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) used at 1/2000 dilution.

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Samples

Lanes 1 & 3 : Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab273530) (0.2 μg)

Lanes 2 & 4 : Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab272107) (0.2 μg)

Green : Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)

Red : Mouse monoclonal [HIS.H8] to 6X His tag® (ab18184)

Lanes 1 & 2 : Green - ab271180 observed at 50 kDa.

Lanes 3 & 4 : Red - loading control Mouse anti-6x HisTag observed at 50 kDa.

ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3% milk before incubation with ab271180 and Mouse anti-6x HisTag overnight at 4 °C at a 1/1000 dilution. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (<a href='/en-us/products/primary-antibodies/rabbit-monoclonal-epr24334-118-to-sars-cov-2-nucleocapsid-protein-ab271180'>ab271180</a>)

All lanes:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)

Observed band size: 50 kDa

false

ELISA

Lab

ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Plates were coated with Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, ab273530) and Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 500 ng/ml.

Primary antibody (ab271180) concentration range 0.045-100 ng /ml.

Pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) used at 1/2000 dilution.

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Samples

Lanes 1 & 3 : Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab273530) (0.1 μg)

Lanes 2 & 4 : Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab272107) (0.1 μg)

Green : Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free (ab280201)

Red : Mouse monoclonal [HIS.H8] to 6X His tag® (ab18184)

Lanes 1 & 2 : Green - ab280201 observed at 50 kDa.

Lanes 3 & 4 : Red - loading control Mouse anti-6x HisTag observed at 50 kDa.

ab280201 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3% milk before incubation with ab271180 and Mouse anti-6x HisTag overnight at 4 °C at a 1/1000 dilution. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [EPR24334-118] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-nucleocapsid-protein-antibody-epr24334-118-bsa-and-azide-free-ab280201'>ab280201</a>)

All lanes:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)

Observed band size: 50 kDa

false

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Green - ab286217 observed at 50 kDa. ab286217 was shown to react with SARS-CoV-2 nucleocapsid in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween ® ) before incubation with ab286217 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Human 800CW secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [F12] - Human (Chimeric) (<a href='/en-us/products/primary-antibodies/sars-cov-2-nucleocapsid-protein-antibody-f12-human-chimeric-ab286217'>ab286217</a>)

All lanes:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)

Secondary

All lanes:

Goat anti-Human 800CW at 1/20000 dilution

false

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

**Lanes 1 - 2 : **Green - ab283894 observed at 51 kDa. ab283894 was shown to react with SARS-CoV-2 N-protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab283894 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Human 800CW secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] (<a href='/en-us/products/primary-antibodies/sars-cov-2-nucleocapsid-protein-antibody-abk79-e11-ab283894'>ab283894</a>)

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530) at 0.2 µg

Lane 2:

Recombinant Human coronavirus SARS Nucleocapsid Protein at 0.2 µg

Secondary

All lanes:

Goat anti-Human 800CW at 1/20000 dilution

false

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

**Lanes 1 - 3 : ** Green - ab271180 observed at 51 kDa.** Lanes 4 - 6 : ** Red - loading control ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) observed at 51 kDa. ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab271180 and ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) overnight at 4 °C at a 1 in 1000 dilution of both antibodies. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Lanes 1 and 4:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530) at 0.1 µg

Lanes 2, 3 and 5:

Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 0.1 µg

Lane 6:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab272107'>ab272107</a>) at 0.1 µg

Observed band size: 51 kDa

false

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Lanes **1 - 2** : Green - ab283895 observed at 51 kDa.ab283895 was shown to react with SARS-CoV-2 N-protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab283894 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Mouse 800CW secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [ABK79-E11] - Mouse IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/sars-cov-2-nucleocapsid-protein-antibody-abk79-e11-mouse-igg2a-chimeric-ab283895'>ab283895</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530) at 0.2 µg

Lane 2:

Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 0.2 µg

Secondary

All lanes:

Goat anti-Mouse 800CW at 1/20000 dilution

false

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

** Lanes 1 - 3 : ** Green - ab271180 observed at 51 kDa.** Lanes 4 - 6 : ** Red - loading control ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) observed at 51 kDa. ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab271180 and ab18184 (Mouse monoclonal [HIS.H8] to 6X His tag®) overnight at 4 °C at a 1 in 1000 dilution of both antibodies. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Lanes 1 and 4:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530) at 0.1 µg

Lanes 2 and 5:

Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 0.1 µg

Lanes 3 and 6:

Observed band size: 51 kDa

false

SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

SDS-PAGE analysis of ab273530.

Supplier Data

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122] - BSA and Azide free - Mouse IgG1 (Chimeric) (ab324891) staining at 1/1000 dilution, shown in black.

In Western blot, ab324891 was shown to bind specifically to SARS-CoV-2 Spike Glycoprotein S2. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1h at room temperature, washed again four times then imaged.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 S Glycoprotein S2 antibody [EPR25038-122] - Mouse IgG1 (Chimeric) - BSA & Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s2-antibody-epr25038-122-mouse-igg1-chimeric-bsa-and-azide-free-ab324891'>ab324891</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s2-fc-chimera-ab272106'>ab272106</a>) at 0.2 µg

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530) at 0.2 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-fc-hrp-preadsorbed-ab98717'>ab98717</a>) at 1/10000 dilution

Observed band size: 135 kDa

false

Exposure time: 10s

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Anti-V5 tag antibody [SV5-P-K] staining at 1/1000 dilution, shown in black. In Western blot, ab206566 was shown to bind specifically to V5 tag. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 3 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) at 1/50000 dilution.

All lanes:

Western blot - Anti-V5 tag antibody [SV5-P-K] (<a href='/en-us/products/primary-antibodies/v5-tag-antibody-sv5-p-k-ab206566'>ab206566</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant E. coli Multi Tag protein (<a href='/en-us/products/proteins-peptides/recombinant-e-coli-multi-tag-protein-ab36791'>ab36791</a>) at 0.2 µg

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530) at 0.2 µg

Observed band size: 45 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122] - BSA and Azide free - Chicken IgY (Chimeric) (ab324893) staining at 1/500 dilution, shown in black.

In Western blot, ab324893 was shown to bind specifically to SARS-CoV-2 Spike Glycoprotein S2. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1h at room temperature, washed again four times then imaged.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122]-Chicken IgY (Chimeric)-BSA&Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s2-antibody-epr25038-122-chicken-igy-chimeric-bsa-and-azide-free-ab324893'>ab324893</a>) at 1/500 dilution

Lane 1:

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530) at 0.2 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/10000 dilution

Observed band size: 135 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122] - BSA and Azide free - Human IgG1 (Chimeric) (ab324889) staining at 1/1000 dilution, shown in black.

In Western blot, ab324889 was shown to bind specifically to SARS-CoV-2 Spike Glycoprotein S2. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1h at room temperature, washed again four times then imaged.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 S Glycoprotein S2 antibody [EPR25038-122] - Human IgG1 (Chimeric) - BSA & Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s2-antibody-epr25038-122-human-igg1-chimeric-bsa-and-azide-free-ab324889'>ab324889</a>) at 1/1000 dilution

Lane 1:

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530) at 0.2 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

Observed band size: 135 kDa

false

Exposure time: 10s

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

Accession

P0DTC9

Animal free

Carrier free

Species

SARS-CoV-2

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 6 - 8 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Every lot is tested by SDS-PAGE. It has also been tested as a control sample by WB with <a href='/en-us/products/primary-antibodies/sars-cov-2-nucleocapsid-protein-antibody-epr24334-118-bsa-and-azide-free-ab280201'>ab280201</a> and ELISA with <a href='/en-us/products/primary-antibodies/rabbit-monoclonal-epr24334-118-to-sars-cov-2-nucleocapsid-protein-ab271180'>ab271180</a>." } } }

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Product details

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment

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Sequence info

[{"sequence":"MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA","proteinLength":"Full Length","predictedMolecularWeight":"48 kDa","actualMolecularWeight":null,"aminoAcidEnd":419,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0DTC9","tags":[{"tag":"8x His","terminus":"C-Terminus"},{"tag":"SKIK","terminus":"N-Terminus"}]}]

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Properties and storage information

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

False

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

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Specifications

Form

Lyophilized

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General info

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.

Sequence similarities

Belongs to the betacoronavirus nucleocapsid protein family.

Post-translational modifications

ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.. Phosphorylated on serine residues by host GSK3A and GSK3B (PubMed:34593624, PubMed:35728038). This promotes the solubility of homodimers that would otherwise aggregate (PubMed:32974389). Host phosphatase would dephosphorylate the protein during assembly at M bound membranes (PubMed:32974389).. Proteolytically cleaved by host CASP6. The cleavage leads to two fragments and facilitates viral replication by inhibiting host IFN signaling. The two fragments may interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information N

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

European journal of pediatrics 184:327 PubMed40332604

2025

Dynamics of Multisystem Inflammatory Syndrome in Children (MIS-C) associated to COVID-19: steady severity despite declining cases and new SARS-CoV-2 variants-a single-center cohort study.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Carzaniga,Valeria Calcaterra,Luca Casiraghi,Tommaso Inzani,Stephana Carelli,Gabriele Del Castillo,Danilo Cereda,Gianvincenzo Zuccotti,Marco Buscaglia

Pathogens and disease 82: PubMed39354682

2024

Differential patterns of antibody response against SARS-CoV-2 nucleocapsid epitopes detected in sera from patients in the acute phase of COVID-19, convalescents, and pre-pandemic individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Razim,Katarzyna Pacyga-Prus,Wioletta Kazana-Płuszka,Agnieszka Zabłocka,Józefa Macała,Hubert Ciepłucha,Andrzej Gamian,Sabina Górska

International journal of microbiology 2024:1331067 PubMed39346702

2024

Development and Evaluation of In-House ELISAs for the Detection of SARS-CoV-2-Specific Antibodies in COVID-19 Patients in Sri Lanka.

Applications

Unspecified application

Species

Unspecified reactive species

Sisira L Pathirana,Bhagya Deepachandi,Peshala Gunasekara,Narmada Fernando,Inoka C Perera,Dakshika Gangani,James Thambyarajah,Dhanushka Dasanayake,Rajiva de Silva,Sunil Premawansa,Andreas Nitsche,Shiroma M Handunnetti

Small (Weinheim an der Bergstrasse, Germany) 19:e2207731 PubMed36916701

2023

Duplex Electrochemical Microfluidic Sensor for COVID-19 Antibody Detection: Natural versus Vaccine-Induced Humoral Response.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Mazzaracchio,Mauricio Rios Maciel,Tatiana Porto Santos,Kazumi Toda-Peters,Amy Q Shen

International journal of molecular sciences 23: PubMed36498845

2022

N-acetylcysteine Reduces Inflammasome Activation Induced by SARS-CoV-2 Proteins In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Milara,Fernando Martínez-Expósito,Paula Montero,Inés Roger,Maria Amparo Bayarri,Pilar Ribera,Miriam Natsuki Oishi-Konari,Jose Ramón Alba-García,Enrique Zapater,Julio Cortijo

Diagnostics (Basel, Switzerland) 12: PubMed36359457

2022

Functionalized Titanium Dioxide Nanoparticle-Based Electrochemical Immunosensor for Detection of SARS-CoV-2 Antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Mohd Abubakar Sadique,Shalu Yadav,Vedika Khare,Raju Khan,Gagan Kant Tripathi,Purnima Swarup Khare

ACS applied bio materials 5:2421-2430 PubMed35522141

2022

Highly Sensitive Electrochemical Immunosensor Platforms for Dual Detection of SARS-CoV-2 Antigen and Antibody based on Gold Nanoparticle Functionalized Graphene Oxide Nanocomposites.

Applications

Unspecified application

Species

Unspecified reactive species

Mohd Abubakar Sadique,Shalu Yadav,Pushpesh Ranjan,Raju Khan,Firoz Khan,Ashok Kumar,Debasis Biswas

View all publications

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Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag)

ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

Reconstitution

Storage buffer

Reactivity data

Product details

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Specifications

Form

General info

Function

Sequence similarities

Post-translational modifications

Product protocols

Target data

Publications (7)

Complementary Products

Primary Antibodies

Proteins & Peptides

Secondary Antibodies

Primary Antibodies

Product promise