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Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) is a SARS-CoV-2 Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >=95% purity and suitable for ELISA, WB, SDS-PAGE.

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Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530), expandable thumbnail
  • Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530), expandable thumbnail
  • Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530), expandable thumbnail
  • Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530), expandable thumbnail
  • Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530), expandable thumbnail

Publications

Key facts

Purity
>=95% SDS-PAGE
Expression system
Escherichia coli
Tags
8x His tag C-Terminus SKIK tag N-Terminus
Applications
ELISA, WB, SDS-PAGE
Biologically active
No

Amino acid sequence

M S D N G P Q N Q R N A P R I T F G G P S D S T G S N Q N G E R S G A R S K Q R R P Q G L P N N T A S W F T A L T Q H G K E D L K F P R G Q G V P I N T N S S P D D Q I G Y Y R R A T R R I R G G D G K M K D L S P R W Y F Y Y L G T G P E A G L P Y G A N K D G I I W V A T E G A L N T P K D H I G T R N P A N N A A I V L Q L P Q G T T L P K G F Y A E G S R G G S Q A S S R S S S R S R N S S R N S T P G S S R G T S P A R M A G N G G D A A L A L L L L D R L N Q L E S K M S G K G Q Q Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K A Y N V T Q A F G R R G P E Q T Q G N F G D Q E L I R Q G T D Y K H W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y T G A I K L D D K D P N F K D Q V I L L N K H I D A Y K T F P P T E P K K D K K K K A D E T Q A L P Q R Q K K Q Q T V T L L P A A D L D D F S K Q L Q Q S M S S A D S T Q A

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

Target data

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed:33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed:34901782, PubMed:36534661). May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed:35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed:35921414). May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury.

Additional Targets

SARS-CoV-2 nucleocapsid protein

Alternative names

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N

Recommended products

Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) is a SARS-CoV-2 Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >=95% purity and suitable for ELISA, WB, SDS-PAGE.

Key facts

Purity
>=95% SDS-PAGE
Expression system
Escherichia coli
Applications
ELISA, WB, SDS-PAGE
Accession
P0DTC9-1
Animal free
No
Species
SARS-CoV-2
Reconstitution
Reconstitute in PBS
Concentration
Loading...
Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

M S D N G P Q N Q R N A P R I T F G G P S D S T G S N Q N G E R S G A R S K Q R R P Q G L P N N T A S W F T A L T Q H G K E D L K F P R G Q G V P I N T N S S P D D Q I G Y Y R R A T R R I R G G D G K M K D L S P R W Y F Y Y L G T G P E A G L P Y G A N K D G I I W V A T E G A L N T P K D H I G T R N P A N N A A I V L Q L P Q G T T L P K G F Y A E G S R G G S Q A S S R S S S R S R N S S R N S T P G S S R G T S P A R M A G N G G D A A L A L L L L D R L N Q L E S K M S G K G Q Q Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K A Y N V T Q A F G R R G P E Q T Q G N F G D Q E L I R Q G T D Y K H W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y T G A I K L D D K D P N F K D Q V I L L N K H I D A Y K T F P P T E P K K D K K K K A D E T Q A L P Q R Q K K Q Q T V T L L P A A D L D D F S K Q L Q Q S M S S A D S T Q A
Accession
P0DTC9
Protein length
Full Length
Predicted molecular weight
48 kDa
Amino acids
1 to 419
Nature
Recombinant
Tags
8x His tag C-Terminus, SKIK tag N-Terminus

Specifications

Form
Lyophilized

General info

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed:33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed:34901782, PubMed:36534661).

Sequence similarities

Belongs to the betacoronavirus nucleocapsid protein family.

Post-translational modifications

ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C

Notes

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

Associated diseases and disorders

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

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