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Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) is a SARS-CoV-2 Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >=95% purity and suitable for ELISA, WB, SDS-PAGE.
Alternative names=Nucleoprotein, N, Nucleocapsid protein, NC, Protein N, N
- Purity
>=95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
ELISA, WB, SDS-PAGE
- Biologically active
No
- Sequence
M S D N G P Q N Q R N A P R I T F G G P S D S T G S N Q N G E R S G A R S K Q R R P Q G L P N N T A S W F T A L T Q H G
K E D L K F P R G Q G V P I N T N S S P D D Q I G Y Y R R A T R R I R G G D G K M K D L S P R W Y F Y Y L G T G P E A G
L P Y G A N K D G I I W V A T E G A L N T P K D H I G T R N P A N N A A I V L Q L P Q G T T L P K G F Y A E G S R G G S
Q A S S R S S S R S R N S S R N S T P G S S R G T S P A R M A G N G G D A A L A L L L L D R L N Q L E S K M S G K G Q Q
Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K A Y N V T Q A F G R R G P E Q T Q G N F G D Q E L I R Q G T D Y K H
W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y T G A I K L D D K D P N F K D Q V I L L N K H I D A Y
K T F P P T E P K K D K K K K A D E T Q A L P Q R Q K K Q Q T V T L L P A A D L D D F S K Q L Q Q S M S S A D S T Q A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Every lot is tested by SDS-PAGE. It has also been tested as a control sample by WB with ab280201 and ELISA with ab271180. |
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Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) is a SARS-CoV-2 Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >=95% purity and suitable for ELISA, WB, SDS-PAGE.
Alternative names=Nucleoprotein, N, Nucleocapsid protein, NC, Protein N, N
Key facts
- Purity
>=95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
ELISA, WB, SDS-PAGE
- Biologically active
No
- Predicted molecular weight
48 kDa
- Amino acids
1 to 419
- Sequence
M S D N G P Q N Q R N A P R I T F G G P S D S T G S N Q N G E R S G A R S K Q R R P Q G L P N N T A S W F T A L T Q H G
K E D L K F P R G Q G V P I N T N S S P D D Q I G Y Y R R A T R R I R G G D G K M K D L S P R W Y F Y Y L G T G P E A G
L P Y G A N K D G I I W V A T E G A L N T P K D H I G T R N P A N N A A I V L Q L P Q G T T L P K G F Y A E G S R G G S
Q A S S R S S S R S R N S S R N S T P G S S R G T S P A R M A G N G G D A A L A L L L L D R L N Q L E S K M S G K G Q Q
Q Q G Q T V T K K S A A E A S K K P R Q K R T A T K A Y N V T Q A F G R R G P E Q T Q G N F G D Q E L I R Q G T D Y K H
W P Q I A Q F A P S A S A F F G M S R I G M E V T P S G T W L T Y T G A I K L D D K D P N F K D Q V I L L N K H I D A Y
K T F P P T E P K K D K K K K A D E T Q A L P Q R Q K K Q Q T V T L L P A A D L D D F S K Q L Q Q S M S S A D S T Q A- Accession
- P0DTC9-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
SARS-CoV-2
- Reconstitution
Reconstitute in Phosphate Buffered saline, aliquot and store at -80 C for 12 months or +4 C for 1 week. Avoid repeated freeze thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Specifications
- Form
Lyophilized
General info
- Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (By similarity). May modulate transforming growth factor-beta signaling by binding host smad3 (By similarity).
- Sequence similarities
Belongs to the betacoronavirus nucleocapsid protein family.
- Post-translational modifications
ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-80°C
Notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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