Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag)
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Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) is a SARS-CoV-2 Full Length protein, in the 1 to 419 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, sELISA.
View Alternative Names
Nucleoprotein, N, Nucleocapsid protein, NC, Protein N
- sELISA
Supplier Data
Sandwich ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB279288)
sELISA analysis of three different batches of ab279288.
Capture and detection antibodies are Mouse monoclonal antibodies that are reactive with the nucleocapsid of SARS-CoV-2. Detection antibody is conjugated to HRP.
Plates were coated overnight with capture antibody (5 μg/ml) in DPB, 100 μl/well at 23°C. Blocked with 1% BSA in DPBS (300 μl/well for 1.5 hours. Washed X3 with PBS + 0.1% Tween 20. Diluted antigen (ab279288) added in DPBS/1% BSA/0.1% Tween 20 at 100 μl/well and incubated for 3.5 hours at 23°C. Washed X3 , as above. Detection antibody (1 μg/ml) added in DPBS/1% BSA/0.1% Tween 20 at 100 μl/well and incubated with shaking for 2 hours at 23°C. Detection with TMB (100 μl/well). Incubated for 10 minutes. Read absorbance at 450/620nm.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB279288)
SDS-PAGE analysis of ab279288 (2.5 μg).
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Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.
Pathways
The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.
Specifications
Form
Liquid
General info
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. Disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation (PubMed : 39225180).
Sequence similarities
Belongs to the betacoronavirus nucleocapsid protein family.
Post-translational modifications
ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.. Phosphorylated on serine residues by host GSK3A and GSK3B (PubMed:34593624, PubMed:35728038). This promotes the solubility of homodimers that would otherwise aggregate (PubMed:32974389). Host phosphatase would dephosphorylate the protein during assembly at M bound membranes (PubMed:32974389).. Proteolytically cleaved by host CASP6. The cleavage leads to two fragments and facilitates viral replication by inhibiting host IFN signaling. The two fragments may interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes.. Acetylated (PubMed:39135075). Deacetylated by host HDAC6 which promotes association of the viral N protein with human G3BP1, disrupting cellular stress granule formation and facilitating viral replication (PubMed:39135075).
Target data
Product promise
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