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Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) is a Fragment protein, in the 1 to 674 aa range, expressed in HEK 293, with >95% purity, < 1 EU/mL endotoxin level and suitable for SDS-PAGE, I-ELISA.

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Images

Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) (AB272105), expandable thumbnail
  • Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) (AB272105), expandable thumbnail
  • Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) (AB272105), expandable thumbnail
  • Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) (AB272105), expandable thumbnail
  • Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) (AB272105), expandable thumbnail

Publications

Key facts

Purity

>95%

Endotoxin level

< 1 EU/mL

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE, I-ELISA

Biologically active

No

Amino acid sequence

M F V F L V L L P L V S S Q C V N L T T R T Q L P P A Y T N S F T R G V Y Y P D K V F R S S V L H S T Q D L F L P F F S N V T W F H A I H V S G T N G T K R F D N P V L P F N D G V Y F A S T E K S N I I R G W I F G T T L D S K T Q S L L I V N N A T N V V I K V C E F Q F C N D P F L G V Y Y H K N N K S W M E S E F R V Y S S A N N C T F E Y V S Q P F L M D L E G K Q G N F K N L R E F V F K N I D G Y F K I Y S K H T P I N L V R D L P Q G F S A L E P L V D L P I G I N I T R F Q T L L A L H R S Y L T P G D S S S G W T A G A A A Y Y V G Y L Q P R T F L L K Y N E N G T I T D A V D C A L D P L S E T K C T L K S F T V E K G I Y Q T S N F R V Q P T E S I V R F P N I T N L C P F G E V F N A T R F A S V Y A W N R K R I S N C V A D Y S V L Y N S A S F S T F K C Y G V S P T K L N D L C F T N V Y A D S F V I R G D E V R Q I A P G Q T G K I A D Y N Y K L P D D F T G C V I A W N S N N L D S K V G G N Y N Y L Y R L F R K S N L K P F E R D I S T E I Y Q A G S T P C N G V E G F N C Y F P L Q S Y G F Q P T N G V G Y Q P Y R V V V L S F E L L H A P A T V C G P K K S T N L V K N K C V N F N F N G L T G T G V L T E S N K K F L P F Q Q F G R D I A D T T D A V R D P Q T L E I L D I T P C S F G G V S V I T P G T N T S N Q V A V L Y Q D V N C T E V P V A I H A D Q L T P T W R V Y S T G S N V F Q T R A G C L I G A E H V N N S Y E C D I P I G A G I C A S Y

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

I-ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Targets

SARS-CoV-2 Spike Glycoprotein S1

Recommended products

Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) is a Fragment protein, in the 1 to 674 aa range, expressed in HEK 293, with >95% purity, < 1 EU/mL endotoxin level and suitable for SDS-PAGE, I-ELISA.

Key facts

Purity

>95%

Endotoxin level

< 1 EU/mL

Expression system

HEK 293 cells

Applications

SDS-PAGE, I-ELISA

Animal free

No

Concentration
Loading...
Storage buffer

pH: 7 - 8
Constituents: PBS

Sequence info

Amino acid sequence

M F V F L V L L P L V S S Q C V N L T T R T Q L P P A Y T N S F T R G V Y Y P D K V F R S S V L H S T Q D L F L P F F S N V T W F H A I H V S G T N G T K R F D N P V L P F N D G V Y F A S T E K S N I I R G W I F G T T L D S K T Q S L L I V N N A T N V V I K V C E F Q F C N D P F L G V Y Y H K N N K S W M E S E F R V Y S S A N N C T F E Y V S Q P F L M D L E G K Q G N F K N L R E F V F K N I D G Y F K I Y S K H T P I N L V R D L P Q G F S A L E P L V D L P I G I N I T R F Q T L L A L H R S Y L T P G D S S S G W T A G A A A Y Y V G Y L Q P R T F L L K Y N E N G T I T D A V D C A L D P L S E T K C T L K S F T V E K G I Y Q T S N F R V Q P T E S I V R F P N I T N L C P F G E V F N A T R F A S V Y A W N R K R I S N C V A D Y S V L Y N S A S F S T F K C Y G V S P T K L N D L C F T N V Y A D S F V I R G D E V R Q I A P G Q T G K I A D Y N Y K L P D D F T G C V I A W N S N N L D S K V G G N Y N Y L Y R L F R K S N L K P F E R D I S T E I Y Q A G S T P C N G V E G F N C Y F P L Q S Y G F Q P T N G V G Y Q P Y R V V V L S F E L L H A P A T V C G P K K S T N L V K N K C V N F N F N G L T G T G V L T E S N K K F L P F Q Q F G R D I A D T T D A V R D P Q T L E I L D I T P C S F G G V S V I T P G T N T S N Q V A V L Y Q D V N C T E V P V A I H A D Q L T P T W R V Y S T G S N V F Q T R A G C L I G A E H V N N S Y E C D I P I G A G I C A S Y

Accession

Protein length

Fragment

Predicted molecular weight

101 kDa

Amino acids

1 to 674

Nature

Recombinant

Tags

Fc tag C-Terminus

Specifications

Form

Liquid

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Predicted molecular weight: 130-140kD, including the Fc tag, Purity >95% SDS-PAGE

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The SARS-CoV-2 Spike Glycoprotein S1 also known as the G10 spike or glycoprotein spike plays an important role in allowing the virus to attach and enter host cells. This protein with a mass of approximately 180 kDa is located on the surface of the virus and forms the outer spikes observed in coronaviruses. Expression of the spike glycoprotein is in virus-infected cells where it facilitates the interaction with host cell receptors. The S1 subunit includes a receptor-binding domain that specifically binds to the human angiotensin-converting enzyme 2 (ACE2) receptors initiating the infection process.

Biological function summary

The spike glycoprotein S1 mediates the fusion of the viral and cellular membranes which is necessary for viral entry. It forms part of a larger trimeric complex comprising S1 and S2 subunits. This complex undergoes conformational changes that drive the membrane fusion process. The glycoprotein contains multiple glycosylation sites which help shield the virus from the host immune response. The proper function and presentation of this glycoprotein are critical for efficient viral spread and infection establishment.

Pathways

The spike glycoprotein S1 is integral to the viral infection pathway and host immune evasion. It interacts with the renin-angiotensin system by binding to the ACE2 receptor disrupting normal receptor activity. This interaction not only facilitates viral entry but also impacts the homeostatic functions typically mediated by ACE2 which include blood pressure regulation. Additionally the spike protein is involved in downstream activation of immune signaling pathways including those related to inflammation and cytokine production which may involve proteins such as IL-6.

Associated diseases and disorders

Infection with the spike glycoprotein S1 is directly related to COVID-19. The binding to ACE2 receptors is linked to the pathology of the disease contributing to respiratory symptoms and in severe cases acute respiratory distress syndrome (ARDS). Through the IL-6 signaling pathway the spike protein is indirectly connected to cytokine release syndrome often observed in severe COVID-19 cases. This connection highlights the importance of targeting this glycoprotein for potential therapeutic interventions and diagnostics such as ELISA SARS-CoV-2 tests and the development of anti-spike antibodies available on platforms like antispark.com for research and clinical purposes.

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