Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera)

• WB

Unknown

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

**Lane 1 : **Red - loading control Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) binding to the sheep Fc Chimera tag on recombinant human coronavirus SARS-CoV-2 spike glycoprotein S2 - observed at 135 kDa **Lanes 2 : **Green - ab283913 observed at 135 kDa. ab283913 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S2 in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) and Donkey anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/20000 dilution. Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122] (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s2-antibody-epr25038-122-ab283913'>ab283913</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (ab272106) at 2 µg

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (ab272106) at 0.2 µg

Secondary

Lane 1:

Western blot - Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/donkey-goat-igg-h-l-irdye-800cw-preadsorbed-ab216775'>ab216775</a>)

Lane 2:

Western blot - Donkey Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/donkey-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216779'>ab216779</a>) at 1/20000 dilution

Observed band size: 135 kDa

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• I-ELISA

Lab

Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

Indirect ELISA showing primary antibody ab323000 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) and Recombinant human coronavirus SARS spike glycoprotein (Tagged, ab270844) at 1000 ng/ml. Binding of ab323000 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1 : 5000.

• I-ELISA

Supplier Data

Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

Indirect ELISA showing primary antibody ab283913 binding SARS-CoV-2 (Omicron variant) Spike Glycoprotein S1 (ab290828); SARS-CoV-2 (Omicron variant) Spike Glycoprotein RBD (ab290829); Recombinant SARS-CoV-2 Omicron variant Spike protein (GCN4-IZ) His tag; Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (ab272106). Antigen concentration is 1000 ng/ml. Substrate solution is p-nitrophenyl phosphate(PNPP). Binding of ab283913 was assessed in a serial dilution range 1000-0 ng/ml. Binding was detected using the secondary antibody, Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

• I-ELISA

Lab

Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

Indirect ELISA showing primary antibody ab323001 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) and Recombinant Human coronavirus SARS spike glycoprotein (Tagged, ab270844) at 1000 ng/ml. Binding of ab323001 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Chicken IgY H&L (HRP, ab6877) was used at 1 : 5000.

• I-ELISA

Lab

Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

Indirect ELISA showing primary antibody ab322272 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105) and Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) at 1000 ng/ml. Binding of ab322272 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1 : 5000.

• I-ELISA

Lab

Indirect ELISA - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

Indirect ELISA showing primary antibody ab322999 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) and Recombinant Human coronavirus SARS spike glycoprotein (Tagged, ab270844) at 1000 ng/ml. Binding of ab322999 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Chicken IgY H&L (HRP, ab6877) was used at 1 : 5000.

• WB

Supplier Data

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122] - BSA and Azide free - Chicken IgY (Chimeric) (ab324893) staining at 1/500 dilution, shown in black.

In Western blot, ab324893 was shown to bind specifically to SARS-CoV-2 Spike Glycoprotein S2. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1h at room temperature, washed again four times then imaged.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122]-Chicken IgY (Chimeric)-BSA&Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s2-antibody-epr25038-122-chicken-igy-chimeric-bsa-and-azide-free-ab324893'>ab324893</a>) at 1/500 dilution

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (ab272106) at 0.2 µg

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.2 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/10000 dilution

Observed band size: 135 kDa

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Exposure time: 10s

• WB

Supplier Data

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122] - BSA and Azide free - Human IgG1 (Chimeric) (ab324889) staining at 1/1000 dilution, shown in black.

In Western blot, ab324889 was shown to bind specifically to SARS-CoV-2 Spike Glycoprotein S2. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1h at room temperature, washed again four times then imaged.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 S Glycoprotein S2 antibody [EPR25038-122] - Human IgG1 (Chimeric) - BSA & Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s2-antibody-epr25038-122-human-igg1-chimeric-bsa-and-azide-free-ab324889'>ab324889</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (ab272106) at 0.2 µg

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.2 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

Observed band size: 135 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122] - BSA and Azide free - Mouse IgG1 (Chimeric) (ab324891) staining at 1/1000 dilution, shown in black.

In Western blot, ab324891 was shown to bind specifically to SARS-CoV-2 Spike Glycoprotein S2. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1h at room temperature, washed again four times then imaged.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 S Glycoprotein S2 antibody [EPR25038-122] - Mouse IgG1 (Chimeric) - BSA & Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s2-antibody-epr25038-122-mouse-igg1-chimeric-bsa-and-azide-free-ab324891'>ab324891</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (ab272106) at 0.2 µg

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.2 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-fc-hrp-preadsorbed-ab98717'>ab98717</a>) at 1/10000 dilution

Observed band size: 135 kDa

false

Exposure time: 10s

• WB

Lab

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

This data was developed using ab283913, the same antibody clone in a different buffer formulation. **Lane 1 : **Red - loading control Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) binding to the sheep Fc Chimera tag on recombinant human coronavirus SARS-CoV-2 spike glycoprotein S2 - observed at 135 kDa **Lanes 2 : **Green - ab283913 observed at 135 kDa. ab283913 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S2 in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) and Donkey anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/20000 dilution. **Blocking buffer : **3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s2-antibody-epr25038-122-bsa-and-azide-free-ab284041'>ab284041</a>)

All lanes:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (ab272106) at 0.2 µg

Secondary

Lane 1:

Western blot - Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/donkey-goat-igg-h-l-irdye-800cw-preadsorbed-ab216775'>ab216775</a>)

Lane 2:

Western blot - Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/donkey-goat-igg-h-l-irdye-800cw-preadsorbed-ab216775'>ab216775</a>) at 1/20000 dilution

false

• Dot

Lab

Dot Blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

Dot blot analysis using ab283913 at 1/1000 dilution followed by a Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution. Lane 1 : Recombinant Human coronavirus SARS-CoV-2 (B.1.1.529/Omicron) Spike Glycoprotein S1 (ab290828). Lane 2 : Recombinant Human coronavirus SARS-CoV-2 (B.1.1.529/Omicron) Spike Glycoprotein S1 RBD protein (ab290829). Lane 3 : Recombinant Human coronavirus SARS-CoV-2 (B.1.1.529/Omicron) Spike protein. Lane 4 : Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (ab272106). Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. Exposure Time : 180 seconds.

• SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (AB272106)

SDS-PAGE analysis of ab272106.