Recombinant Human coronavirus SARS Nucleocapsid Protein (Tagged) is a SARS-CoV Fragment protein, in the 1 to 49 aa range, expressed in Escherichia coli, with >95% purity and suitable for ELISA, WB, SDS-PAGE.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes May appear as multiple bands on SDS-PAGE (intact protein at 31.6 kDa, GST fusion protein at 26 kDa and the nucleocapsid fragment at 5.6 kDa). |
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455).
9a, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N
Recombinant Human coronavirus SARS Nucleocapsid Protein (Tagged) is a SARS-CoV Fragment protein, in the 1 to 49 aa range, expressed in Escherichia coli, with >95% purity and suitable for ELISA, WB, SDS-PAGE.
Constituents: 50% Glycerol (glycerin, glycerine), 0.8% Tris HCl, 0.35% Sodium chloride
GS-4B Sepharose-Affinity Purification.
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication (PubMed:17210170). May modulate transforming growth factor-beta signaling by binding host SMAD3 (PubMed:18055455).
Belongs to the betacoronavirus nucleocapsid protein family.
Proteolytically cleaved by host CASP6. The cleavage leads to two fragments and facilitates viral replication by inhibiting host IFN signaling. The two fragments may interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes.
Contains the nucleocapsid protein immunodominant region. Contains GST fusion partner. Immunoreactive with SARS positive sera.
The nucleocapsid protein of SARS-CoV-2 also known as N protein or COVID-19 nucleocapsid protein is important for the viral replication and packaging processes. It has a mass of approximately 43-46 kDa and is expressed in the cytoplasm of infected host cells. This protein is highly conserved among coronaviruses and plays a significant role in the viral lifecycle by binding to the viral RNA genome thereby facilitating its encapsulation within the virion. Moreover it aids in stabilizing the viral RNA structure and is essential for ribonucleoprotein complex formation.
The nucleocapsid protein of SARS-CoV-2 interacts directly with other viral components and host cellular machinery to orchestrate efficient viral assembly and release. It forms a ribonucleoprotein complex by self-association and binding to the RNA genome promoting virion integrity. This protein also plays a part in modulating host cell responses potentially by interfering with antiviral signaling pathways ensuring that the virus evades the host immune system to some degree.
Many scientific studies show that the SARS-CoV-2 nucleocapsid protein is involved in the viral replication and assembly pathways. It interacts with viral proteins such as the membrane (M) protein and possibly others to facilitate these processes. The protein also exhibits connectivity with cellular pathways related to RNA processing and metabolism due to its RNA-binding capabilities further influencing viral replication efficiency and host-pathogen interactions.
The nucleocapsid protein has a significant association with COVID-19 the disease caused by SARS-CoV-2. Its expression correlates with viral pathogenicity and immune response dysregulation which can lead to severe outcomes in infected individuals. The N protein has links with comorbid conditions potentially affecting disease progression. Furthermore the nucleocapsid protein interacts with host proteins like G3BP1 and other cellular factors that may contribute to immune modulation influencing the clinical symptoms associated with COVID-19.
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