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AB116193

Recombinant Human COTL1 protein

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Recombinant Human COTL1 protein is a Human Full Length protein, in the 1 to 142 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CLP, COTL1, Coactosin-like protein

1 Images
SDS-PAGE - Recombinant Human COTL1 protein (AB116193)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human COTL1 protein (AB116193)

15% SDS-PAGE showing ab116193 at approximately 18.1kDa (3µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q14019

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMATKIDKEACRAAYNLVRDDGSAVIWVTFKYDGSTIVPGEQGAEYQHFIQQCTDDVRLFAFVRFTTGDAMSKRSKFALITWIGENVSGLQRAKTGTDKTLVKEVVQNFAKEFVISDRKELEEDFIKSELKKAGGANYDAQTE","proteinLength":"Full Length","predictedMolecularWeight":"18.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":142,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q14019","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COTL1 also known as Coactosin-like protein is a small protein of approximately 16 kDa. This protein is expressed in a variety of tissues with notable presence in hematopoietic cells such as leukocytes. COTL1 binds actin and is involved mechanically in modulating actin polymerization. This protein also interacts with arachidonate 5-lipoxygenase (ALOX5) facilitating its enzymatic activity which plays an important role in lipid metabolism.
Biological function summary

COTL1 plays an important role in the regulation of immune responses and inflammatory processes. It acts as a cofactor stabilizing the interaction between ALOX5 and actin filaments impacting the formation of leukotrienes which mediate immune responses. COTL1 is not known to be part of a larger protein complex but it influences the function of ALOX5 suggesting a collaborative role in cellular processes related to inflammation.

Pathways

COTL1 influences the biosynthesis of leukotrienes within the arachidonic acid metabolic pathway. This pathway is significant for inflammatory response modulation. COTL1 and ALOX5 together affect the production of leukotrienes providing a connection to the eicosanoid signaling pathway. This involvement positions COTL1 as an important regulator of inflammation.

COTL1 has connections with inflammatory disorders and certain cancers. The protein's interaction with ALOX5 links it to conditions such as asthma where leukotriene pathways play a part in pathogenesis. Overexpression or dysregulation of COTL1 can also relate to specific cancers including leukemia where inflammatory signaling contributes to disease progression. Understanding the COTL1-protein interactions may offer therapeutic potential.
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Specifications

Form

Liquid

Additional notes

ab116193 was purified using conventional chromatography techniques.

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General info

Function

Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis.

Sequence similarities

Belongs to the actin-binding proteins ADF family. Coactosin subfamily.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis.
See full target information COTL1
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