Recombinant human Coxsackie Adenovirus Receptor/hCAR protein (His tag) is a Human Fragment protein, in the 20 to 237 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Publications
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- Molecular biology reports 49:3213-32232022Human Coxsackie- and adenovirus receptor is a putative target of neutrophil elastase-mediated shedding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeonie Herrmann,Louise Schelletter,Raimund Hoffrogge,Karsten Niehaus,Volker Rudolph,Martin FarrPubMed 35122600
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
L S I T T P E E M I E K A K G E T A Y L P C K F T L S P E D Q G P L D I E W L I S P A D N Q K V D Q V I I L Y S G D K I Y D D Y Y P D L K G R V H F T S N D L K S G D A S I N V T N L Q L S D I G T Y Q C K V K K A P G V A N K K I H L V V L V K P S G A R C Y V D G S E E I G S D F K I K C E P K E G S L P L Q Y E W Q K L S D S Q K M P T S W L A E M T S S V I S V K N A S S E Y S G T Y S C T V R N R V G S D Q C L L R L N V V P P S N K A G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes The protein has a calculated MW of 24.9 kDa. The protein migrates as 32-35 kDa under reducing condition (SDS-PAGE) due to glycosylation. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. (Microbial infection) Acts as a receptor for adenovirus type C. (Microbial infection) Acts as a receptor for Coxsackievirus B1 to B6.
Alternative names
CAR, CXADR, Coxsackievirus and adenovirus receptor, hCAR, CVB3-binding protein, Coxsackievirus B-adenovirus receptor, HCVADR
Recommended products
Recombinant human Coxsackie Adenovirus Receptor/hCAR protein (His tag) is a Human Fragment protein, in the 20 to 237 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by the ability of immobilized ab168893 to support the adhesion of mouse neutrophils. When 50000 cells/well are added to Coxsackie Adenovirus Receptor coated plates (4 µg/mL and 100 µL/well ), approximately 20% - 50% will adhere specifically after 60 minutes at 37°C.
- Accession
- P78310-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS
Sequence info
Amino acid sequence
- L S I T T P E E M I E K A K G E T A Y L P C K F T L S P E D Q G P L D I E W L I S P A D N Q K V D Q V I I L Y S G D K I Y D D Y Y P D L K G R V H F T S N D L K S G D A S I N V T N L Q L S D I G T Y Q C K V K K A P G V A N K K I H L V V L V K P S G A R C Y V D G S E E I G S D F K I K C E P K E G S L P L Q Y E W Q K L S D S Q K M P T S W L A E M T S S V I S V K N A S S E Y S G T Y S C T V R N R V G S D Q C L L R L N V V P P S N K A G
- Accession
- P78310
- Protein length
- Fragment
- Predicted molecular weight
- 24.87 kDa
- Amino acids
- 20 to 237
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as Coxsackie Adenovirus Receptor
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1 product image
SDS-PAGE - Recombinant human Coxsackie Adenovirus Receptor/hCAR protein (His tag) (ab168893)
SDS-PAGE analysis of ab168893. Stained overnight with Coomassie Blue.
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