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AB153194

Recombinant Human CPEB3 protein (GST tag N-Terminus)

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Recombinant Human CPEB3 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 697 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

KIAA0940, CPEB3, Cytoplasmic polyadenylation element-binding protein 3, CPE-BP3, CPE-binding protein 3, hCPEB-3

1 Images
SDS-PAGE - Recombinant Human CPEB3 protein (GST tag N-Terminus) (AB153194)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CPEB3 protein (GST tag N-Terminus) (AB153194)

ab153194 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q8NE35

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MQDDLLMDKSKTQPQPQQQQRQQQQPQPESSVSEAPSTPLSSETPKPEENSAVPALSPAAAPPAPNGPDKMQMESPLLPGLSFHQPPQQPPPPQEPAAPGASLSPSFGSTWSTGTTNAVEDSFFQGITPVNGTMLFQNFPHHVNPVFGGTFSPQIGLAQTQHHQQPPPPAPAPQPAQPAQPPQAQPPQQRRSPASPSQAPYAQRSAAAAYGHQPIMTSKPSSSSAVAAAAAAAAASSASSSWNTHQSVNAAWSAPSNPWGGLQAGRDPRRAVGVGVGVGVGVPSPLNPISPLKKPFSSNVIAPPKFPRAAPLTSKSWMEDNAFWTDNGNNLLPFQDRSRPYDTFNLHSLENSLMDMIRTDHEPLKGKHYPPSGPPMSFADIMWRNHFAGRMGINFHHPGTDNIMALNNAFLDDSHGDQALSSGLSSPTRCQNGERVERYSRKVFVGGLPPDIDEDEITASFRRFGPLVVDWPHKAESKSYFPPKGYAFLLFQEESSVQALIDACLEEDGKLYLCVSSPIKDKPVQIRPWNLSDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHNDIDKRVEVKPYVLDDQMCDECQGTRCGGKFAPFFCANVTCLQYYCEYCWASIHSRAGREFHKPLVKEGGDRPRHVPFRWS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":697,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8NE35","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytoplasmic polyadenylation element-binding protein 3 (CPEB3) also called CPEB-3 functions as an RNA-binding protein that regulates mRNA translation. It has a mass of about 74 kDa. CPEB3 is predominantly expressed in neuronal tissues within the brain particularly in the hippocampus. It controls cytoplasmic polyadenylation which is important for the regulation of mRNA stability and translation. By binding to specific sequences in mRNA CPEB3 influences the polyadenylation status of that mRNA affecting synthesis of proteins important for synaptic function and plasticity.
Biological function summary

CPEB3 acts as an important regulator of synaptic plasticity and long-term memory formation. It belongs to the CPEB family known for their role in controlling translation of target mRNAs in dendrites. This protein interacts with other molecules and possibly forms part of a larger protein complex to exert its functions. Its ability to influence mRNA translation links it to processes facilitating structural and functional changes at synaptic junctions which are essential for learning and memory.

Pathways

CPEB3 is integral to signaling cascades that govern synaptic plasticity. It functions in pathways like the mTOR and MAPK signaling pathways. In these it coordinates with proteins like mTOR and ERK to regulate translation of mRNAs needed for dendritic growth and synapse strengthening. These pathways highlight the role of CPEB3 in adjusting neuron responsiveness during synaptic transmission and plasticity.

CPEB3 connections suggest roles in neurological conditions such as Alzheimer's disease and schizophrenia. Disruptions in CPEB3 function have potential links to cognitive decline observed in these diseases. Alzheimer’s disease in particular involves proteins like Tau with which CPEB3 may have indirect interactions through affected pathways. In schizophrenia altered synaptic plasticity mechanisms involving CPEB3 could contribute to the disorder's cognitive and behavioral symptoms.
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Specifications

Form

Liquid

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General info

Function

Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator (By similarity). In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3'-UTR, but binds to a U-rich loop within a stem-loop structure (By similarity). Required for the consolidation and maintenance of hippocampal-based long term memory (By similarity). In the basal state, binds to the mRNA 3'-UTR of the glutamate receptors GRIA2/GLUR2 mRNA and negatively regulates their translation (By similarity). Also represses the translation of DLG4, GRIN1, GRIN2A and GRIN2B (By similarity). When activated, acts as a translational activator of GRIA1 and GRIA2 (By similarity). In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation (By similarity). Binds to the 3'-UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception (By similarity). Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation (By similarity). Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay (PubMed : 21336257). In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance (PubMed : 20639532). In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression (PubMed : 26398195).

Sequence similarities

Belongs to the RRM CPEB family.

Post-translational modifications

Activated by NEURL1-mediated monoubiquitination, resulting in the growth of new dendritic spines and increased levels of GRIA1 and GRIA2. NEURL1-mediated monoubiquitination facilitates synaptic plasticity and hippocampal-dependent memory storage.. Under basal unstimulated conditions when CPEB3 is mainly unaggregated, sumoylated and acts as a translational repressor. Following neuronal stimulation, becomes desumoylated and aggregated which is required for the translation of mRNA targets and for dendritic filopodia formation.. Following neuronal stimulation, cleaved by CAPN2 which abolishes its translational repressor activity, leading to translation of CPEB3 target mRNAs.. Phosphorylation is enhanced by neuronal stimulation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator (By similarity). In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3'-UTR, but binds to a U-rich loop within a stem-loop structure (By similarity). Required for the consolidation and maintenance of hippocampal-based long term memory (By similarity). In the basal state, binds to the mRNA 3'-UTR of the glutamate receptors GRIA2/GLUR2 mRNA and negatively regulates their translation (By similarity). Also represses the translation of DLG4, GRIN1, GRIN2A and GRIN2B (By similarity). When activated, acts as a translational activator of GRIA1 and GRIA2 (By similarity). In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation (By similarity). Binds to the 3'-UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception (By similarity). Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation (By similarity). Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay (PubMed : 21336257). In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance (PubMed : 20639532). In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression (PubMed : 26398195).
See full target information CPEB3
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