Recombinant Human CPI-17 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A A Q R L G K R V L S K L Q S P S R A R G P G G S P G G L Q K R H A R V T V K Y D R R E L Q R R L D V E K W I D G R L E E L Y R G M E A D M P D E I N I D E L L E L E S E E E R S R K I Q G L L K S C G K P V E D F I Q E L L A K L Q G L H R Q P G L R Q P S P S H D G S L S P L Q D R A R T A H P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Alternative names
CPI17, PPP1INL, PPP1R14A, Protein phosphatase 1 regulatory subunit 14A, 17 kDa PKC-potentiated inhibitory protein of PP1, Protein kinase C-potentiated inhibitor protein of 17 kDa, CPI-17
Recommended products
Recombinant Human CPI-17 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q96A00-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris, 0.0584% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A A Q R L G K R V L S K L Q S P S R A R G P G G S P G G L Q K R H A R V T V K Y D R R E L Q R R L D V E K W I D G R L E E L Y R G M E A D M P D E I N I D E L L E L E S E E E R S R K I Q G L L K S C G K P V E D F I Q E L L A K L Q G L H R Q P G L R Q P S P S H D G S L S P L Q D R A R T A H P
- Accession
- Q96A00
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab75206 is purified by using conventional chromatography techniques.
General info
- Function
Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
- Sequence similarities
Belongs to the PP1 inhibitor family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CPI-17 also known as Protein Kinase C-Potentiated Inhibitor Protein-17 is a protein that acts as a myosin phosphatase inhibitor. It has a molecular mass of approximately 17 kDa. Expressed in various tissues including smooth muscle heart and brain CPI-17 plays an essential role in regulating smooth muscle contractility. The protein modulates the phosphorylation state of myosin light chains by inhibiting myosin phosphatase activity impacting muscle contraction dynamics.
- Biological function summary
CPI-17 influences smooth muscle function by modulating the phosphorylation status of myosin light chains. It is not a component of larger protein complexes but interacts directly with myosin phosphatase. The precise regulation of myosin light chain phosphorylation by CPI-17 is critical for maintaining smooth muscle contractile responses affecting functions such as vasoconstriction and peristalsis.
- Pathways
CPI-17 plays an important role in the RhoA/ROCK signaling pathway which is key to controlling smooth muscle tension and motility. Through this pathway CPI-17 interacts closely with proteins like ROCK (Rho-associated coiled-coil containing protein kinase). Additionally CPI-17 is associated with the PKC (Protein Kinase C) pathway where its phosphorylation status and inhibitory activity are regulated by PKC enzymes emphasizing its role in cellular signal transduction.
- Associated diseases and disorders
CPI-17 is implicated in conditions such as hypertension and asthma where dysregulated smooth muscle contractility is a factor. In hypertension CPI-17's interaction with the PKC pathway suggests a substantial influence on blood vessel tension while in asthma altered CPI-17 activity affects airway smooth muscle contraction. The link between CPI-17 and PKC proteins under these conditions provides insight into potential therapeutic targets for managing these diseases.
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1 product image
SDS-PAGE - Recombinant Human CPI-17 protein (ab75206)
15% SDS-PAGE showing ab75206 at approximately 18kDa. (3μg)
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