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AB79148

Recombinant Human CPLX1 protein

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Recombinant Human CPLX1 protein is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Complexin-1, Complexin I, Synaphin-2, CPX I, CPLX1

1 Images
SDS-PAGE - Recombinant Human CPLX1 protein (AB79148)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human CPLX1 protein (AB79148)

15% SDS-PAGE showing ab79148 (3μg).
Note : Real molecular weight on SDS-PAGE is shifted up compared to predicted molecular weight.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O14810

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMEFVMKQALGGATKDMGKMLGGDEEKDPDAAKKEEERQEALRQAEEERKAKYAKMEAEREAVRQGIRDKYGIKKKEEREAEAQAAMEANSEGSLTRPKKAIPPGCGDEVEEEDESILDTVIKYLPGPLQDMLKK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O14810","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CPLX1 also known as Complexin-1 functions in the mechanical control of presynaptic vesicle release. It weighs approximately 16 kDa and finds expression primarily in the central nervous system. CPLX1 interacts with the SNARE complex playing a role in neurotransmitter release. This protein ensures precise regulation at synaptic junctions which are important for efficient neuronal communication.
Biological function summary

Complexin-1 acts as an essential regulator of synaptic exocytosis. It stabilizes the SNARE complex inhibiting premature synaptic vesicle fusion while also preparing the vesicles for rapid release upon calcium influx. In synaptic terminals Complexin-1 ensures quick and precise neurotransmitter release therefore supporting synaptic plasticity and signal transduction essential for learning and memory. Complexin-1 forms complexes with proteins like synaptobrevin and syntaxin participating in the excitatory and inhibitory synaptic transmission.

Pathways

Complexin-1 plays a significant role in the synaptic vesicle cycle and neurotransmitter secretion pathways. It interacts with proteins in the SNARE complex notably SNAP-25 and synaptotagmin facilitating neurotransmitter release upon stimulus. Its function in these pathways supports the maintenance of synaptic efficacy and neuronal circuitry critical for normal nervous system operation and responses to environmental stimuli.

Abnormalities in Complexin-1 expression or function link to neurological conditions like epilepsy and schizophrenia. Both of these disorders involve disrupted synaptic transmission and altered neurotransmitter release. Additionally Complexin-1 has connections with the protein alpha-synuclein implicated in neurodegenerative diseases such as Parkinson's disease. These associations highlight the importance of Complexin-1 in maintaining synaptic function and its potential as a therapeutic target.
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Specifications

Form

Liquid

Additional notes

ab79148 is purified using conventional chromatography techniques.Endotoxin level: < 1.0 EU per 1 μg of protein (determined by LAL method)

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General info

Function

Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles (PubMed : 21785414). Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse (PubMed : 21785414). Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.

Sequence similarities

Belongs to the complexin/synaphin family.

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Product protocols

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Target data

Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles (PubMed : 21785414). Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse (PubMed : 21785414). Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.
See full target information CPLX1
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