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AB140737

Recombinant Human CPSF30/NAR protein (denatured) (His tag N-Terminus)

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Recombinant Human CPSF30/NAR protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 244 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

CPSF30, NAR, NEB1, CPSF4, Cleavage and polyadenylation specificity factor subunit 4, Cleavage and polyadenylation specificity factor 30 kDa subunit, NS1 effector domain-binding protein 1, No arches homolog, CPSF 30 kDa subunit, Neb-1

1 Images
SDS-PAGE - Recombinant Human CPSF30/NAR protein (denatured) (His tag N-Terminus) (AB140737)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human CPSF30/NAR protein (denatured) (His tag N-Terminus) (AB140737)

15% SDS-PAGE analysis of ab140737 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O95639

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as CPSF30
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYLVGFCPEGPSCKFMHPRFELPMGTTEQPPLPQQTQPPAKQRTPQVIGVMQSQNSSAGNRGPRPLEQVTCYKCGEKGHYANRCTKGHLAFLSGQ","proteinLength":"Full Length","predictedMolecularWeight":"29.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":244,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95639","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPSF30 also known as Cleavage and Polyadenylation Specificity Factor subunit 30 is a protein involved in the cleavage and polyadenylation of pre-mRNA. It has a mass of about 30 kDa and performs its functions inside the cell nucleus. CPSF30 is expressed widely across various tissues enabling it to play multiple roles in RNA processing. Another notable alias for this protein is NAR (Nuclear Apoptosis Regulatory protein) which highlights its involvement in apoptotic processes.
Biological function summary

CPSF30 helps in mRNA processing by forming part of the CPSF complex. This complex is important for cutting and adding a poly-A tail to the 3' end of pre-mRNAs a step essential for mRNA stability export and translation. CPSF30 interacts directly with other CPSF subunits such as CPSF73 and CPSF100 enabling the PCAS endonuclease activity required for cleaving pre-mRNA. This activity facilitates proper gene expression regulation in response to different cellular conditions.

Pathways

CPSF30 is an integral part of the post-transcriptional regulation of gene expression. The protein participates in both the mRNA polyadenylation pathway and mRNA surveillance pathway ensuring accurate mRNA maturation and degradation of faulty mRNA. Through these pathways CPSF30 interacts with related proteins such as FIP1 and CSTF77 which assist in the precise control of gene expression by assuring proper RNA transcript modification and functionality.

CPSF30 involvement links to specific health conditions related to RNA processing defects and apoptosis. Disorders including certain types of cancer and autoimmune diseases have been associated with abnormalities in CPSF30 function. The CPSF complex's interaction with apoptotic regulators like BCL2L11 suggests its potential role in conditions where apoptosis is dysregulated. Understanding these interactions may offer insights into the mechanistic pathways contributing to these diseases providing potential targets for therapeutic intervention.
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Specifications

Form

Liquid

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General info

Function

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).

Sequence similarities

Belongs to the CPSF4/YTH1 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).
See full target information CPSF4
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Complementary Products

Primary Antibodies

AB248378

Anti-CPSF30/NAR antibody [EPR7433] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-CPSF30/NAR antibody [EPR7433] - BSA and Azide free (AB248378)

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