Recombinant Human CPTP protein is a Human Full Length protein, in the 1 to 214 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M D D S E T G F N L K V V L V S F K Q C L D E K E E V L L D P Y I A S W K G L V R F L N S L G T I F S F I S K D V V S K L R I M E R L R G G P Q S E H Y R S L Q A M V A H E L S N R L V D L E R R S H H P E S G C R T V L R L H R A L H W L Q L F L E G L R T S P E D A R T S A L C A D S Y N A S L A A Y H P W V V R R A V T V A F C T L P T R E V F L E A M N V G P P E Q A V Q M L G E A L P F I Q R V Y N V S Q K L Y A E H S L L D L P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mediates the intracellular transfer of ceramide-1-phosphate (C1P) between organelle membranes and the cell membrane. Required for normal structure of the Golgi stacks. Can bind phosphoceramides with a variety of aliphatic chains, but has a preference for lipids with saturated C16:0 or monounsaturated C18:1 aliphatic chains, and is inefficient with phosphoceramides containing lignoceryl (C24:0). Plays a role in the regulation of the cellular levels of ceramide-1-phosphate, and thereby contributes to the regulation of phospholipase PLA2G4A activity and the release of arachidonic acid. Has no activity with galactosylceramide, lactosylceramide, sphingomyelin, phosphatidylcholine, phosphatidic acid and ceramide. C1P transfer is stimulated by phosphatidylserine in C1P source vesicles (PubMed:28011644). Regulates autophagy, inflammasome mediated IL1B and IL18 processing, and pyroptosis, but not apoptosis (PubMed:29164996).
Alternative names
GLTPD1, CPTP, Ceramide-1-phosphate transfer protein, Glycolipid transfer protein domain-containing protein 1, GLTP domain-containing protein 1
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Recombinant Human CPTP protein is a Human Full Length protein, in the 1 to 214 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q5TA50-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M D D S E T G F N L K V V L V S F K Q C L D E K E E V L L D P Y I A S W K G L V R F L N S L G T I F S F I S K D V V S K L R I M E R L R G G P Q S E H Y R S L Q A M V A H E L S N R L V D L E R R S H H P E S G C R T V L R L H R A L H W L Q L F L E G L R T S P E D A R T S A L C A D S Y N A S L A A Y H P W V V R R A V T V A F C T L P T R E V F L E A M N V G P P E Q A V Q M L G E A L P F I Q R V Y N V S Q K L Y A E H S L L D L P
- Accession
- Q5TA50
- Protein length
- Full Length
- Predicted molecular weight
- 26.8 kDa
- Amino acids
- 1 to 214
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab187436 was purified using conventional chromatography techniques.
General info
- Function
Mediates the intracellular transfer of ceramide-1-phosphate (C1P) between organelle membranes and the cell membrane. Required for normal structure of the Golgi stacks. Can bind phosphoceramides with a variety of aliphatic chains, but has a preference for lipids with saturated C16:0 or monounsaturated C18:1 aliphatic chains, and is inefficient with phosphoceramides containing lignoceryl (C24:0). Plays a role in the regulation of the cellular levels of ceramide-1-phosphate, and thereby contributes to the regulation of phospholipase PLA2G4A activity and the release of arachidonic acid. Has no activity with galactosylceramide, lactosylceramide, sphingomyelin, phosphatidylcholine, phosphatidic acid and ceramide. C1P transfer is stimulated by phosphatidylserine in C1P source vesicles (PubMed:28011644). Regulates autophagy, inflammasome mediated IL1B and IL18 processing, and pyroptosis, but not apoptosis (PubMed:29164996).
- Sequence similarities
Belongs to the GLTP family.
- Subcellular localisation
- Endosome membrane, Nucleus outer membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as GLTPD1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CPTP also known as ceramide-1-phosphate transfer protein is a specialized protein that facilitates the transfer of ceramide-1-phosphate (C1P) between membranes. CPTP has a molecular mass of approximately 25 kDa. You can find it widely expressed in tissues such as liver lung and kidney indicating its significant functional roles across different organ systems. Its structural characteristics make it a critical facilitator in the local distribution of lipids contributing to cellular lipid homeostasis.
- Biological function summary
The transfer of C1P by CPTP plays an important role in various signaling pathways and cellular processes. While it's not a component of a larger complex CPTP functions independently to mediate the intermembrane transport of its lipid substrate. This activity influences processes such as cell proliferation inflammation and apoptosis. Through its interaction with C1P CPTP significantly impacts cellular responses to stress and external stimuli serving as a vital regulator of cell fate.
- Pathways
CPTP and its associated lipid transport function integrate into essential biological routes such as the sphingolipid signaling pathway and the peroxisome proliferator-activated receptor (PPAR) signaling pathway. In these pathways C1P collaborates with proteins like sphingosine kinases and cPLA2 to affect cellular responses. The transport activity of CPTP ensures the precise localization of C1P providing a fine-tuning mechanism necessary for maintaining pathway integrity and function.
- Associated diseases and disorders
Dysregulation of CPTP activity has been linked to conditions such as inflammatory diseases and metabolic disorders. Inflammatory processes might get disrupted with altered CPTP function due to its regulation of lipid mediators of inflammation. Additionally abnormal lipid signaling through proteins related to CPTP like cPLA2 may contribute to the pathogenesis of metabolic syndrome. Further understanding of CPTP's disease associations offers the potential for novel therapeutic targets to mitigate these conditions.
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1 product image
SDS-PAGE - Recombinant Human CPTP protein (ab187436)
15% SDS-PAGE analysis of ab187436 (3μg).
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