Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged)
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Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) is a Human Full Length protein, in the 1 to 1140 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE, FuncS.
View Alternative Names
RNF75, ROC1, RBX1, E3 ubiquitin-protein ligase RBX1, E3 ubiquitin-protein transferase RBX1, Protein ZYP, RING finger protein 75, RING-box protein 1, Regulator of cullins 1, Rbx1
- FuncS
Supplier Data
Functional Studies - Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (AB271467)
Cereblon-BRD3 interaction.
Various amounts of ab271467 were incubated in 10-ul reaction with dBET1 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (AB271467)
SDS-PAGE analysis of ab271467.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The DDB1 CRBN and associated proteins interact closely in the ubiquitin-proteasome system functioning as a part of the larger cullin-RING ligase complex. It acts by recognizing and binding target proteins tagging them for degradation which mediates cellular processes such as DNA repair cell cycle and apoptosis. DDB1 acts as an adaptor that recruits substrate-specific receptors and CRBN often serves as a specificity factor within the protein assembly.
Pathways
The DDB1-CRBN complex intricately integrates into the ubiquitin-proteasome pathway and the cell cycle regulation pathway. It is important for the removal of misfolded or damaged proteins preventing protein aggregation. It interacts with proteins such as c-JUN and MDM2 which link it to cell cycle regulation and stress response pathways. This complex functions in substrate recognition and subsequent degradation to maintain cellular equilibrium.
Specifications
Form
Liquid
General info
Function
E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair (PubMed : 10230407, PubMed : 10579999, PubMed : 11961546, PubMed : 15983046, PubMed : 16678110, PubMed : 19112177, PubMed : 19679664, PubMed : 22748924, PubMed : 23455478, PubMed : 27565346, PubMed : 29769719, PubMed : 33417871, PubMed : 38326650). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (PubMed : 27565346). The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 35982156). Core component of a Cul9-RING ubiquitin ligase complex composed of CUL9 and RBX1, which mediates mono-ubiquitination of p53/TP53 (PubMed : 38605244). Recruits the E2 ubiquitin-conjugating enzyme CDC34 to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. As part of a multisubunit complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5; polyubiquitinates monoubiquitinated POLR2A (PubMed : 19920177).
Sequence similarities
Belongs to the RING-box family.
Subcellular localisation
Nucleus
Target data
Additional targets
Complementary Products
Product promise
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