Recombinant Human CRBN protein is a Human Full Length protein, in the 1 to 442 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
M A G E G D Q Q D A A H N M G N H L P L L P A E S E E E D E M E V E D Q D S K E A K K P N I I N F D T S L P T S H T Y L G A D M E E F H G R T L H D D D S C Q V I P V L P Q V M M I L I P G Q T L P L Q L F H P Q E V S M V R N L I Q K D R T F A V L A Y S N V Q E R E A Q F G T T A E I Y A Y R E E Q D F G I E I V K V K A I G R Q R F K V L E L R T Q S D G I Q Q A K V Q I L P E C V L P S T M S A V Q L E S L N K C Q I F P S K P V S R E D Q C S Y K W W Q K Y Q K R K F H C A N L T S W P R W L Y S L Y D A E T L M D R I K K Q L R E W D E N L K D D S L P S N P I D F S Y R V A A C L P I D D V L R I Q L L K I G S A I Q R L R C E L D I M N K C T S L C C K Q C Q E T E I T T K N E I F S L S L C G P M A A Y V N P H G Y V H E T L T V Y K A C N L N L I G R P S T E H S W F P G Y A W T V A Q C K I C A S H I G W K F T A T K K D M S P Q K F W G L T R S A L L P T I P D T E D E I S P D K V I L C L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 (Probable). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed:20223979, PubMed:24328678, PubMed:25043012, PubMed:25108355). Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons (PubMed:18414909, PubMed:29530986). Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1 (PubMed:18414909). May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism (By similarity).
Alternative names
Protein cereblon, CRBN, AD-006
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Recombinant Human CRBN protein is a Human Full Length protein, in the 1 to 442 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Alternative names
Protein cereblon, CRBN, AD-006
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- Q96SW2-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M A G E G D Q Q D A A H N M G N H L P L L P A E S E E E D E M E V E D Q D S K E A K K P N I I N F D T S L P T S H T Y L G A D M E E F H G R T L H D D D S C Q V I P V L P Q V M M I L I P G Q T L P L Q L F H P Q E V S M V R N L I Q K D R T F A V L A Y S N V Q E R E A Q F G T T A E I Y A Y R E E Q D F G I E I V K V K A I G R Q R F K V L E L R T Q S D G I Q Q A K V Q I L P E C V L P S T M S A V Q L E S L N K C Q I F P S K P V S R E D Q C S Y K W W Q K Y Q K R K F H C A N L T S W P R W L Y S L Y D A E T L M D R I K K Q L R E W D E N L K D D S L P S N P I D F S Y R V A A C L P I D D V L R I Q L L K I G S A I Q R L R C E L D I M N K C T S L C C K Q C Q E T E I T T K N E I F S L S L C G P M A A Y V N P H G Y V H E T L T V Y K A C N L N L I G R P S T E H S W F P G Y A W T V A Q C K I C A S H I G W K F T A T K K D M S P Q K F W G L T R S A L L P T I P D T E D E I S P D K V I L C L
- Accession
- Protein length
Full Length
- Predicted molecular weight
76.9 kDa
- Amino acids
1 to 442
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Glutathione Sepharose 4 Fast Flow
General info
- Function
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 (Probable). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed:20223979, PubMed:24328678, PubMed:25043012, PubMed:25108355). Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons (PubMed:18414909, PubMed:29530986). Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1 (PubMed:18414909). May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism (By similarity).
- Sequence similarities
Belongs to the CRBN family.
- Post-translational modifications
Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Protein concentration is above or equal to 0.05 mg/ml.
Supplementary info
- Activity summary
CRBN also known as cereblon is a protein involved in the regulation of protein homeostasis. It has a molecular weight of approximately 51 kDa. CRBN is expressed in various tissues including the brain kidney and muscle. It functions as a substrate receptor for the CRL4-CRBN E3 ubiquitin ligase complex recognizing proteins for ubiquitination and degradation. The chemical structure of thalidomide shows binding affinity to CRBN influencing its function.
- Biological function summary
Cereblon plays a role in the regulation of protein stability and degradation. It forms part of the CRL4-CRBN complex which tags specific proteins for ubiquitination and subsequent proteasomal degradation. This function is critical for maintaining cellular protein balance. Additionally CRBN modulates immune response and has immunomodulator properties influencing cell-mediated responses.
- Pathways
CRBN participates in the ubiquitin-proteasome pathway which regulates protein turnover and quality control within cells. Moreover CRBN is involved in immune signaling pathways partly by modulating the expression of various cytokines and immune-related proteins. It interacts with proteins such as DDB1 and CUL4 within these pathways impacting cellular responses to stress and immune challenges.
- Associated diseases and disorders
CRBN is associated with multiple myeloma and intellectual disability. Cereblon acts as a target for drugs like thalidomide and lenalidomide which are used in the treatment of multiple myeloma by altering ubiquitination processes. In intellectual disability alterations in CRBN function or expression can disrupt neuronal development and signaling. CRBN interacts with proteins like IKZF1 and IKZF3 within these contexts affecting their roles in disease progression and treatment responses.
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1 product image
SDS-PAGE - Recombinant Human CRBN protein (ab162449)
ab162449 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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