Recombinant Human CRBN protein (His tag)
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(1 Publication)
Recombinant Human CRBN protein (His tag) is a Human Full Length protein, in the 1 to 442 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
View Alternative Names
AD-006, CRBN, Protein cereblon
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human CRBN protein (His tag) (AB235611)
ab235611 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Cereblon plays a role in the regulation of protein stability and degradation. It forms part of the CRL4-CRBN complex which tags specific proteins for ubiquitination and subsequent proteasomal degradation. This function is critical for maintaining cellular protein balance. Additionally CRBN modulates immune response and has immunomodulator properties influencing cell-mediated responses.
Pathways
CRBN participates in the ubiquitin-proteasome pathway which regulates protein turnover and quality control within cells. Moreover CRBN is involved in immune signaling pathways partly by modulating the expression of various cytokines and immune-related proteins. It interacts with proteins such as DDB1 and CUL4 within these pathways impacting cellular responses to stress and immune challenges.
Specifications
Form
Liquid
Additional notes
Purified from an invitro E.coli expression system.
General info
Function
Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL (PubMed : 26990986, PubMed : 33009960). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed : 20223979, PubMed : 24328678, PubMed : 25043012, PubMed : 25108355). Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons (PubMed : 18414909, PubMed : 29530986). Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1 (PubMed : 18414909). May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism (By similarity). Plays a negative role in TLR4 signaling by interacting with TRAF6 and ECSIT, leading to inhibition of ECSIT ubiquitination, an important step of the signaling (PubMed : 31620128).
Sequence similarities
Belongs to the CRBN family.
Post-translational modifications
Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.
Subcellular localisation
Nucleus
Target data
Publications (1)
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Journal of enzyme inhibition and medicinal chemistry 37:1715-1723 PubMed35698881
2022
Applications
Unspecified application
Species
Unspecified reactive species
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