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AB152297

Recombinant Human CRF1/CRHR1 protein (GST tag N-Terminus)

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Recombinant Human CRF1/CRHR1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 415 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CRFR, CRFR1, CRHR, CRHR1, Corticotropin-releasing factor receptor 1, CRF-R-1, CRF-R1, CRFR-1, Corticotropin-releasing hormone receptor 1, CRH-R-1, CRH-R1

1 Images
SDS-PAGE - Recombinant Human CRF1/CRHR1 protein (GST tag N-Terminus) (AB152297)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CRF1/CRHR1 protein (GST tag N-Terminus) (AB152297)

12.5% SDS-PAGE analysis of ab152297 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P34998

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB77686

Anti-CRF1/CRHR1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CRF1/CRHR1 antibody (AB77686)

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Proteins & Peptides

AB152782

Recombinant Human UCP1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human UCP1 protein (GST tag N-Terminus) (AB152782)

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  • 0
Proteins & Peptides

AB269103

Recombinant human UBE2I / UBC9 protein (Active)

Functional Studies - Recombinant human UBE2I / UBC9 protein (Active) (AB269103)

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Proteins & Peptides

AB79512

Recombinant Human UCP3 protein

SDS-PAGE - Recombinant Human UCP3 protein (AB79512)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as CRF1.
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Sequence info

[{"sequence":"MGGHPQLRLVKALLLLGLNPVSASLQDQHCESLSLASNISGLQCNASVDLIGTCWPRSPAGQLVVRPCPAFFYGVRYNTTNNGYRECLANGSWAARVNYSECQEILNEEKKSKVHYHVAVIINYLGHCISLVALLVAFVLFLRLRSIRCLRNIIHWNLISAFILRNATWFVVQLTMSPEVHQSNVGWCRLVTAAYNYFHVTNFFWMFGEGCYLHTAIVLTYSTDRLRKWMFICIGWGVPFPIIVAWAIGKLYYDNEKCWFGKRPGVYTDYIYQGPMILVLLINFIFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSFLESFQGFFVSVFYCFLNSEVRSAIRKRWHRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAV","proteinLength":"Full Length","predictedMolecularWeight":"74.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":415,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P34998","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRF1 also known as CRHR1 is a receptor for corticotropin-releasing factor (CRF) a peptide hormone involved in stress response. This receptor is a G protein-coupled receptor with a mass of approximately 50 kDa. CRHR1 is expressed in the central nervous system especially in the brain regions like the cortex and the cerebellum. It can also be found in peripheral tissues like the pituitary gland. Its localization is important because it is involved in mediating the effects of CRF across various tissues.
Biological function summary

CRHR1 plays a significant role in stress-related responses and is involved in the modulation of the hypothalamic-pituitary-adrenal (HPA) axis. When CRF binds to this receptor it triggers the release of adrenocorticotropic hormone (ACTH) from the pituitary gland. CRHR1 is also part of the CRF receptor family complex which includes CRHR2 another receptor with similar functions. The interaction with CRF can initiate various signaling pathways further affecting physiological responses to stress.

Pathways

CRHR1 integrates into the HPA axis pathway as well as the cyclic AMP (cAMP) signaling pathway. In the HPA pathway CRHR1's activation leads to the secretion of ACTH ultimately resulting in cortisol production by the adrenal glands. The cAMP-responsive element-binding protein (CREB) may become activated in response to CRHR1 modulation influencing gene expression and cellular response to stress. The CRHR1 receptor therefore plays a critical role in these pathways by linking stress signals to hormonal and behavioral changes.

CRHR1 has a strong connection to stress-related conditions such as depression and anxiety. Dysregulation in CRHR1 signaling can lead to altered HPA axis functioning which contributes to the development of these disorders. Stress-induced activation of CRHR1 is associated with increased levels of cortisol which is commonly seen in individuals with depression. Furthermore CRHR1 interacts closely with other proteins like the glucocorticoid receptor (GR) which also play a part in these psychiatric conditions indicating a complex network of interactions that govern stress responses.
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Specifications

Form

Liquid

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General info

Function

G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Post-translational modifications

C-terminal Ser or Thr residues may be phosphorylated.. Phosphorylation at Ser-301 by PKA prevents maximal coupling to Gq-protein, and thereby negatively regulates downstream signaling.

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Product protocols

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Target data

G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.
See full target information CRHR1
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Product promise

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