Recombinant Human CRIF1 protein is a Human Fragment protein, in the 48 to 222 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M P R W Q L G P R Y A A K Q F A R Y G A A S G V V P G S L W P S P E Q L R E L E A E E R E W Y P S L A T M Q E S L R V K Q L A E E Q K R R E R E Q H I A E C M A K M P Q M I V N W Q Q Q Q R E N W E K A Q A D K E R R A R L Q A E A Q E L L G Y Q V D P R S A R F Q E L L Q D L E K K E R K R L K E E K Q K R K K E A R A A A L A A A V A Q D P A A S G A P S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.
Alternative names
MRPL59, PLINP1, PRG6, GADD45GIP1, Large ribosomal subunit protein mL64, CKII beta-associating protein, CR6-interacting factor 1, Growth arrest and DNA damage-inducible proteins-interacting protein 1, Papillomavirus L2-interacting nuclear protein 1, p53-responsive gene 6 protein, MRP-L59, CRIF1, PLINP, PLINP-1
Recommended products
Recombinant Human CRIF1 protein is a Human Fragment protein, in the 48 to 222 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q8TAE8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M P R W Q L G P R Y A A K Q F A R Y G A A S G V V P G S L W P S P E Q L R E L E A E E R E W Y P S L A T M Q E S L R V K Q L A E E Q K R R E R E Q H I A E C M A K M P Q M I V N W Q Q Q Q R E N W E K A Q A D K E R R A R L Q A E A Q E L L G Y Q V D P R S A R F Q E L L Q D L E K K E R K R L K E E K Q K R K K E A R A A A L A A A V A Q D P A A S G A P S S
- Accession
- Q8TAE8
- Protein length
- Fragment
- Predicted molecular weight
- 22.6 kDa
- Amino acids
- 48 to 222
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab113863 was purified by using conventional chromatography techniques.
General info
- Function
Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.
- Sequence similarities
Belongs to the mitochondrion-specific ribosomal protein mL64 family.
- Subcellular localisation
- Mitochondrion, Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as GADD45GIP1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CRIF1 also known as CRIFone is a protein implicated in coordinating mitochondrial translation and metabolism. It has a molecular weight of approximately 33 kDa. The gene encoding CRIF1 is found in humans and is expressed in various tissues including liver heart and skeletal muscle indicating its significant role in energy-demanding tissues. CRIF1 localizes mainly in the mitochondrial inner membrane where it interacts with mitoribosomes to facilitate the synthesis and integration of respiratory chain complexes.
- Biological function summary
CRIF1 functionally contributes to the mitochondrial protein synthesis machinery and is a part of the mitoribosome complex. It directly interacts with nascent polypeptide chains during mitochondrial translation ensuring proper insertion into the mitochondrial inner membrane. This interaction is essential for effective cellular respiration and ATP production. The protein further plays a role in maintaining mitochondrial structure and regulating cellular energy homeostasis by stabilizing key mitochondrial complexes particularly involved in oxidative phosphorylation.
- Pathways
CRIF1 participates noticeably in the oxidative phosphorylation and mitochondrial biogenesis pathways. In these pathways CRIF1 ensures the proper assembly and maintenance of the electron transport chain complexes. It interacts with other mitochondrial proteins such as COX1 and ND1 integral parts of Complex IV and Complex I of the electron transport chain respectively. These interactions position CRIF1 as critical for efficient energy production and metabolic regulation.
- Associated diseases and disorders
CRIF1 has been linked to metabolic syndromes and neurodegenerative diseases. Its dysregulation can impact mitochondrial function leading to energy production deficits seen in disorders like mitochondrial myopathies and Parkinson's disease. CRIF1 interacts with proteins like Parkin known to be involved in mitochondrial quality control processes. Disruptions in CRIF1 function can exacerbate defects in mitochondrial maintenance and turnover contributing to disease pathogenesis.
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1 product image
SDS-PAGE - Recombinant Human CRIF1 protein (ab113863)
ab113863 at 3 μg analysed by 15% SDS-PAGE.
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