Recombinant human Cripto1/CRIPTO protein (Fc Chimera) is a Human Fragment protein, in the 31 to 169 aa range, expressed in HEK 293, with >95% purity and suitable for FuncS, SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
T h e o r e t i c a l s e q u e n c e : L G H Q E F A R P S R G Y L A F R D D S I W P Q E E P A I R P R S S Q R V P P M G I Q H S K E L N R T C C L N G G T C M L G S F C A C P P S F Y G R N C E H D V R K E N C G S V P H D T W L P K K C S L C K C W H G Q L R C F P Q A F L P G C D G L V M D E H L V A S R T P E L P P S G S S N T K V D K K V E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Under reducing conditions ab84062 migrates as a broad band between 45 and 55 kDa on SDS-PAGE due to post-translational modifications, in |
Target data
Function
GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953).
Alternative names
CRIPTO-1, TDGF1, CRIPTO, Protein Cripto, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1, CRGF
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Recombinant human Cripto1/CRIPTO protein (Fc Chimera) is a Human Fragment protein, in the 31 to 169 aa range, expressed in HEK 293, with >95% purity and suitable for FuncS, SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- 200 ng/ml ab84062 induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells
- Accession
- P13385-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in 0.5 mL of PBS
- Concentration
- Storage buffer
Constituents: 10% Trehalose, 1% Human serum albumin
Sequence info
Amino acid sequence
- T h e o r e t i c a l s e q u e n c e : L G H Q E F A R P S R G Y L A F R D D S I W P Q E E P A I R P R S S Q R V P P M G I Q H S K E L N R T C C L N G G T C M L G S F C A C P P S F Y G R N C E H D V R K E N C G S V P H D T W L P K K C S L C K C W H G Q L R C F P Q A F L P G C D G L V M D E H L V A S R T P E L P P S G S S N T K V D K K V E P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
- Accession
- P13385
- Protein length
- Fragment
- Amino acids
- 31 to 169
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953).
- Sequence similarities
Belongs to the EGF-CFC (Cripto-1/FRL1/Cryptic) family.
- Post-translational modifications
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (TMEM8A), removing an acyl-chain at the sn-2 position of GPI and releasing CRIPTO as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (GPLD1) into a soluble form.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Reconstitute for long term storage
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
200 ng/ml of this Chimera induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells. This product was previously labelled as Cripto1
This product was previously labelled as Cripto1
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3 product images
SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)
Lane 1 – MW markers; Lane 2 – ab84062; Lane 3 – ab84062 treated with PNGase F to remove potential N linked glycans; Lane 4 – ab84062 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.
SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)
A sample of ab84062 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4 20% Tris-HCl 2D gel. Approximately 40 μg of protein was loaded; Gel was stained using Deep Purple™. The spot train indicates the presence of multiple glycoforms. Spots within the spot train were cut from the gel and identified as Cripto1/CRIPTO (Fc Chimera) by protein mass fingerprinting.
Functional Studies - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)
Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.
The triangle indicates theoretical pI and MW of the protein.
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