JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190395

Recombinant human Cripto1/CRIPTO protein (Tagged)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human Cripto1/CRIPTO protein (Tagged) is a Human Full Length protein, in the 31 to 150 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

CRIPTO-1, TDGF1, CRIPTO, Protein Cripto, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1, CRGF

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELSA. Immobilized recombinant Human Nodal protein at 2μg/ml (100μl/well) can bind rhCripto-1 with a linear range of 0.5-100 ng/ml.

Accession

P13385

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as Cripto1

This product was previously labelled as Cripto1

style
left-column

Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHGNLYFQGGEFLGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPPMGIQHSKELNRTCCLNGGTCMLGSFCACPPSFYGRNCEHDVRKENCGSVPHDTWLPKKCSLCKCWHGQLRCFPQAFLPGCD","proteinLength":"Full Length","predictedMolecularWeight":"16.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":150,"aminoAcidStart":31,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P13385","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

ab190395 was refolded using a unique temperature shift inclusion body refolding technology and chromatographically purified.

style
left-column

General info

Function

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed : 27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed : 11909953).

Sequence similarities

Belongs to the EGF-CFC (Cripto-1/FRL1/Cryptic) family.

Post-translational modifications

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (TMEM8A), removing an acyl-chain at the sn-2 position of GPI and releasing CRIPTO as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (GPLD1) into a soluble form.

style
left-column

Product protocols

style
left-column

Target data

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed : 27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed : 11909953).
See full target information CRIPTO
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB108391

Anti-Cripto1/CRIPTO antibody [EPNCIR106A]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

  • 0
  • 0
Proteins & Peptides

AB101548

Recombinant human ULK3 protein

Functional Studies - Recombinant human ULK3 protein (AB101548)

  • 0
  • 0
Proteins & Peptides

AB177262

Recombinant human WNK1 protein (Tagged)

Functional Studies - Recombinant human WNK1 protein (Tagged) (AB177262)

  • 0
  • 0
Proteins & Peptides

AB198644

Native Cynomolgus monkey Transferrin protein

SDS-PAGE - Native Cynomolgus monkey Transferrin protein (AB198644)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com