Recombinant Human Crk p38 protein is a Human Full Length protein, in the 1 to 224 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A G N F D S E E R S S W Y W G R L S R Q E A V A L L Q G Q R H G V F L V R D S S T S P G D Y V L S V S E N S R V S H Y I I N S S G P R P P V P P S P A Q P P P G V S P S R L R I G D Q E F D S L P A L L E F Y K I H Y L D T T T L I E P V S R S R Q G S G V I L R Q E E A E Y V R A L F D F N G N D E E D L P F K K G D I L R I R D K P E E Q W W N A E D S E G K R G M I P V P Y V E K Y R P A S A S V S A L I G G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Isoform Crk-II. Regulates cell adhesion, spreading and migration (PubMed:31311869). Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4 (PubMed:19004829). May regulate the EFNA5-EPHA3 signaling (By similarity).
Alternative names
Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK
Recommended products
Recombinant Human Crk p38 protein is a Human Full Length protein, in the 1 to 224 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P46108-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A G N F D S E E R S S W Y W G R L S R Q E A V A L L Q G Q R H G V F L V R D S S T S P G D Y V L S V S E N S R V S H Y I I N S S G P R P P V P P S P A Q P P P G V S P S R L R I G D Q E F D S L P A L L E F Y K I H Y L D T T T L I E P V S R S R Q G S G V I L R Q E E A E Y V R A L F D F N G N D E E D L P F K K G D I L R I R D K P E E Q W W N A E D S E G K R G M I P V P Y V E K Y R P A S A S V S A L I G G R
- Accession
- P46108
- Protein length
- Full Length
- Predicted molecular weight
- 25 kDa
- Amino acids
- 1 to 224
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab101046 was purified by using conventional chromatography.
General info
- Function
Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.
- Sequence similarities
Belongs to the CRK family.
- Post-translational modifications
Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway (PubMed:17515907). Isoform Crk-II: Phosphorylated by KIT (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Crk p38 protein (ab101046)
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