Recombinant Human CRMP1 protein is a Human Full Length protein, in the 1 to 572 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S E F M S Y Q G K K S I P H I T S D R L L I K G G R I I N D D Q S L Y A D V Y L E D G L I K Q I G E N L I V P G G V K T I E A N G R M V I P G G I D V N T Y L Q K P S Q G M T A A D D F F Q G T R A A L V G G T T M I I D H V V P E P G S S L L T S F E K W H E A A D T K S C C D Y S L H V D I T S W Y D G V R E E L E V L V Q D K G V N S F Q V Y M A Y K D V Y Q M S D S Q L Y E A F T F L K G L G A V I L V H A E N G D L I A Q E Q K R I L E M G I T G P E G H A L S R P E E L E A E A V F R A I T I A G R I N C P V Y I T K V M S K S A A D I I A L A R K K G P L V F G E P I A A S L G T D G T H Y W S K N W A K A A A F V T S P P L S P D P T T P D Y L T S L L A C G D L Q V T G S G H C P Y S T A Q K A V G K D N F T L I P E G V N G I E E R M T V V W D K A V A T G K M D E N Q F V A V T S T N A A K I F N L Y P R K G R I A V G S D A D V V I W D P D K L K T I T A K S H K S A V E Y N I F E G M E C H G S P L V V I S Q G K I V F E D G N I N V N K G M G R F I P R K A F P E H L Y Q R V K I R N K V F G L Q G V S R G M Y D G P V Y E V P A T P K Y A T P A P S A K S S P S K H Q P P P I R N L H Q S N F S L S G A Q I D D N N P R R T G H R I V A P P G G R S N I T S L G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton (PubMed:25358863). Plays a role in axon guidance (PubMed:25358863). During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone (PubMed:25358863). Involved in invasive growth and cell migration (PubMed:11562390). May participate in cytokinesis (PubMed:19799413).
Alternative names
DPYSL1, ULIP3, CRMP1, Dihydropyrimidinase-related protein 1, DRP-1, Collapsin response mediator protein 1, Inactive dihydropyrimidinase, Unc-33-like phosphoprotein 3, CRMP-1, ULIP-3
Recommended products
Recombinant Human CRMP1 protein is a Human Full Length protein, in the 1 to 572 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q14194-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00348% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S E F M S Y Q G K K S I P H I T S D R L L I K G G R I I N D D Q S L Y A D V Y L E D G L I K Q I G E N L I V P G G V K T I E A N G R M V I P G G I D V N T Y L Q K P S Q G M T A A D D F F Q G T R A A L V G G T T M I I D H V V P E P G S S L L T S F E K W H E A A D T K S C C D Y S L H V D I T S W Y D G V R E E L E V L V Q D K G V N S F Q V Y M A Y K D V Y Q M S D S Q L Y E A F T F L K G L G A V I L V H A E N G D L I A Q E Q K R I L E M G I T G P E G H A L S R P E E L E A E A V F R A I T I A G R I N C P V Y I T K V M S K S A A D I I A L A R K K G P L V F G E P I A A S L G T D G T H Y W S K N W A K A A A F V T S P P L S P D P T T P D Y L T S L L A C G D L Q V T G S G H C P Y S T A Q K A V G K D N F T L I P E G V N G I E E R M T V V W D K A V A T G K M D E N Q F V A V T S T N A A K I F N L Y P R K G R I A V G S D A D V V I W D P D K L K T I T A K S H K S A V E Y N I F E G M E C H G S P L V V I S Q G K I V F E D G N I N V N K G M G R F I P R K A F P E H L Y Q R V K I R N K V F G L Q G V S R G M Y D G P V Y E V P A T P K Y A T P A P S A K S S P S K H Q P P P I R N L H Q S N F S L S G A Q I D D N N P R R T G H R I V A P P G G R S N I T S L G
- Accession
- Q14194
- Protein length
- Full Length
- Predicted molecular weight
- 64.8 kDa
- Amino acids
- 1 to 572
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab202599 was purified using conventional chromatography techniques.
General info
- Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton (PubMed:25358863). Plays a role in axon guidance (PubMed:25358863). During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone (PubMed:25358863). Involved in invasive growth and cell migration (PubMed:11562390). May participate in cytokinesis (PubMed:19799413).
- Sequence similarities
Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.
- Post-translational modifications
Phosphorylation at Ser-522 enhances CRMP1-mediated alteration of FLNA ternary structure and FLNA dissociation from F-actin.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome, Spindle
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Terms & Conditions.
2 product images
SDS-PAGE - Recombinant Human CRMP1 protein (ab202599)
15% SDS-PAGE analysis of 3 μg ab202599.
Western blot - Recombinant Human CRMP1 protein (ab202599)
Blocking and dilution buffer: 5% NFDM /TBST
All lanes: Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (Anti-CRMP1 + CRMP2 antibody [EPR7792] ab129082) at 1/1000 dilution
Lanes 1 and 3: Western blot - Recombinant Human CRMP1 protein (ab202599)
Lanes 2 and 4: Western blot - Recombinant Human CRMP2 protein (Recombinant Human CRMP2 protein ab114269)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Exposure time: 3s
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Product protocols
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