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Recombinant Human CRMP2 protein is a Human Full Length protein, in the 1 to 572 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.

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Images

SDS-PAGE - Recombinant Human CRMP2 protein (AB114269), expandable thumbnail
  • Western blot - Recombinant Human CRMP2 protein (AB114269), expandable thumbnail

Key facts

Expression system
Wheat germ
Tags
GST tag N-Terminus
Applications
SDS-PAGE, ELISA, WB
Biologically active
No

Amino acid sequence

M S Y Q G K K N I P R I T S D R L L I K G G K I V N D D Q S F Y A D I Y M E D G L I K Q I G E N L I V P G G V K T I E A H S R M V I P G G I D V H T R F Q M P D Q G M T S A D D F F Q G T K A A L A G G T T M I I D H V V P E P G T S L L A A F D Q W R E W A D S K S C C D Y S L H V D I S E W H K G I Q E E M E A L V K D H G V N S F L V Y M A F K D R F Q L T D C Q I Y E V L S V I R D I G A I A Q V H A E N G D I I A E E Q Q R I L D L G I T G P E G H V L S R P E E V E A E A V N R A I T I A N Q T N C P L Y I T K V M S K S S A E V I A Q A R K K G T V V Y G E P I T A S L G T D G S H Y W S K N W A K A A A F V T S P P L S P D P T T P D F L N S L L S C G D L Q V T G S A H C T F N T A Q K A V G K D N F T L I P E G T N G T E E R M S V I W D K A V V T G K M D E N Q F V A V T S T N A A K V F N L Y P R K G R I A V G S D A D L V I W D P D S V K T I S A K T H N S S L E Y N I F E G M E C R G S P L V V I S Q G K I V L E D G T L H V T E G S G R Y I P R K P F P D F V Y K R I K A R S R L A E L R G V P R G L Y D G P V C E V S V T P K T V T P A S S A K T S P A K Q Q A P P V R N L H Q S G F S L S G A Q I D D N I P R R T T Q R I V A P P G G R A N I T S L G

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

(for recombinant protein)

Target data

Function

Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.

Alternative names

Recommended products

Recombinant Human CRMP2 protein is a Human Full Length protein, in the 1 to 572 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.

Key facts

Expression system
Wheat germ
Applications
SDS-PAGE, ELISA, WB
Accession
Q16555-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione

Sequence info

Amino acid sequence

M S Y Q G K K N I P R I T S D R L L I K G G K I V N D D Q S F Y A D I Y M E D G L I K Q I G E N L I V P G G V K T I E A H S R M V I P G G I D V H T R F Q M P D Q G M T S A D D F F Q G T K A A L A G G T T M I I D H V V P E P G T S L L A A F D Q W R E W A D S K S C C D Y S L H V D I S E W H K G I Q E E M E A L V K D H G V N S F L V Y M A F K D R F Q L T D C Q I Y E V L S V I R D I G A I A Q V H A E N G D I I A E E Q Q R I L D L G I T G P E G H V L S R P E E V E A E A V N R A I T I A N Q T N C P L Y I T K V M S K S S A E V I A Q A R K K G T V V Y G E P I T A S L G T D G S H Y W S K N W A K A A A F V T S P P L S P D P T T P D F L N S L L S C G D L Q V T G S A H C T F N T A Q K A V G K D N F T L I P E G T N G T E E R M S V I W D K A V V T G K M D E N Q F V A V T S T N A A K V F N L Y P R K G R I A V G S D A D L V I W D P D S V K T I S A K T H N S S L E Y N I F E G M E C R G S P L V V I S Q G K I V L E D G T L H V T E G S G R Y I P R K P F P D F V Y K R I K A R S R L A E L R G V P R G L Y D G P V C E V S V T P K T V T P A S S A K T S P A K Q Q A P P V R N L H Q S G F S L S G A Q I D D N I P R R T T Q R I V A P P G G R A N I T S L G
Accession
Q16555
Protein length
Full Length
Predicted molecular weight
88.99 kDa
Amino acids
1 to 572
Nature
Recombinant
Tags
GST tag N-Terminus

Specifications

Form
Liquid

General info

Function

Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.

Sequence similarities

Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.

Post-translational modifications

3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.

Subcellular localisation
Cytoskeleton

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant Human CRMP2 protein (ab114269), expandable thumbnail

    SDS-PAGE - Recombinant Human CRMP2 protein (ab114269)

    12.5% SDS-PAGE analysis of ab114269.
    Stained with Coomassie Blue.

  • Western blot - Recombinant Human CRMP2 protein (ab114269), expandable thumbnail

    Western blot - Recombinant Human CRMP2 protein (ab114269)

    Blocking and dilution buffer: 5% NFDM /TBST

    All lanes: Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (Anti-CRMP1 + CRMP2 antibody [EPR7792] ab129082) at 1/1000 dilution

    Lanes 1 and 3: Western blot - Recombinant Human CRMP1 protein (Recombinant Human CRMP1 protein ab202599)

    Lanes 2 and 4: Western blot - Recombinant Human CRMP2 protein (ab114269)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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