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AB114269

Recombinant Human CRMP2 protein

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Recombinant Human CRMP2 protein is a Human Full Length protein, in the 1 to 572 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CRMP2, ULIP2, DPYSL2, Dihydropyrimidinase-related protein 2, DRP-2, Collapsin response mediator protein 2, N2A3, Unc-33-like phosphoprotein 2, CRMP-2, ULIP-2

2 Images
Western blot - Recombinant Human CRMP2 protein (AB114269)
  • WB

Lab

Western blot - Recombinant Human CRMP2 protein (AB114269)

Blocking and dilution buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (<a href='/en-us/products/primary-antibodies/crmp1-crmp2-antibody-epr7792-ab129082'>ab129082</a>) at 1/1000 dilution

Lanes 1 and 3:

Western blot - Recombinant Human CRMP1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-crmp1-protein-ab202599'>ab202599</a>)

Lanes 2 and 4:

Western blot - Recombinant Human CRMP2 protein (ab114269)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 3s

SDS-PAGE - Recombinant Human CRMP2 protein (AB114269)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CRMP2 protein (AB114269)

12.5% SDS-PAGE analysis of ab114269.
Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

Q16555

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(for recombinant protein)</p>" } } }
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Sequence info

[{"sequence":"MSYQGKKNIPRITSDRLLIKGGKIVNDDQSFYADIYMEDGLIKQIGENLIVPGGVKTIEAHSRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMIIDHVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVDISEWHKGIQEEMEALVKDHGVNSFLVYMAFKDRFQLTDCQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILDLGITGPEGHVLSRPEEVEAEAVNRAITIANQTNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG","proteinLength":"Full Length","predictedMolecularWeight":"88.99 kDa","actualMolecularWeight":null,"aminoAcidEnd":572,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q16555","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.

Sequence similarities

Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.

Post-translational modifications

3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.. Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding leading to destabilization of microtubule assembly in axons and neurodegeneration (By similarity). Phosphorylation by DYRK2 at Ser-522 is required for subsequent phosphorylation by GSK3B.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.
See full target information DPYSL2
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Complementary Products

Primary Antibodies

AB129082

Anti-CRMP1 + CRMP2 antibody [EPR7792]

RabMAb|Recombinant|Trial Size

Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (AB129082)

  • 0
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Reagents, Controls & Accessories

AB210899

ELISA Coating Buffer 1X

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Primary Antibodies

AB252953

Anti-Aldolase antibody [EPR23181-39]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Western blot - Anti-Aldolase antibody [EPR23181-39] (AB252953)

  • 0
  • 0
Primary Antibodies

AB216329

Anti-RAGE antibody [EPR21171]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-RAGE antibody [EPR21171] (AB216329)

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Product promise

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