Recombinant human CSF-1-R protein is a Human Fragment protein, in the 20 to 512 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
I P V I E P S V P E L V V K P G A T V T L R C V G N G S V E W D G P P S P H W T L Y S D G S S S I L S T N N A T F Q N T G T Y R C T E P G D P L G G S A A I H L Y V K D P A R P W N V L A Q E V V V F E D Q D A L L P C L L T D P V L E A G V S L V R V R G R P L M R H T N Y S F S P W H G F T I H R A K F I Q S Q D Y Q C S A L M G G R K V M S I S I R L K V Q K V I P G P P A L T L V P A E L V R I R G E A A Q I V C S A S S V D V N F D V F L Q H N N T K L A I P Q Q S D F H N N R Y Q K V L T L N L D Q V D F Q H A G N Y S C V A S N V Q G K H S T S M F F R V V E S A Y L N L S S E Q N L I Q E V T V G E G L N L K V M V E A Y P G L Q G F N W T Y L G P F S D H Q P E P K L A N A T T K D T Y R H T F T L S L P R L K P S E A G R Y S F L A R N P G G W R A L T F E L T L R Y P P E V S V I W T F I N G S G T L L C A A S G Y P Q P N V T W L Q C S G H T D R C D E A Q V L Q V W D D P Y P E V L S Q E P F H K V T V Q S L L T V E T L E H N Q T Y E C R A H N S V G S G S W A F I P I S A G A H T H P P D E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Calculated MW 55.1 kDa. DTT-reduced protein migrates as 80-95 kDa polypeptide in SDS-PAGE. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).
Alternative names
CD115, FMS, CSF1R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R
Recommended products
Recombinant human CSF-1-R protein is a Human Fragment protein, in the 20 to 512 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Activity measured by its ability to inhibit the MCSF-induced proliferation of NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.5-8.7 pg/ml in the presence of 1 ng/ml of rhMCSF.
- Accession
- P07333-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- I P V I E P S V P E L V V K P G A T V T L R C V G N G S V E W D G P P S P H W T L Y S D G S S S I L S T N N A T F Q N T G T Y R C T E P G D P L G G S A A I H L Y V K D P A R P W N V L A Q E V V V F E D Q D A L L P C L L T D P V L E A G V S L V R V R G R P L M R H T N Y S F S P W H G F T I H R A K F I Q S Q D Y Q C S A L M G G R K V M S I S I R L K V Q K V I P G P P A L T L V P A E L V R I R G E A A Q I V C S A S S V D V N F D V F L Q H N N T K L A I P Q Q S D F H N N R Y Q K V L T L N L D Q V D F Q H A G N Y S C V A S N V Q G K H S T S M F F R V V E S A Y L N L S S E Q N L I Q E V T V G E G L N L K V M V E A Y P G L Q G F N W T Y L G P F S D H Q P E P K L A N A T T K D T Y R H T F T L S L P R L K P S E A G R Y S F L A R N P G G W R A L T F E L T L R Y P P E V S V I W T F I N G S G T L L C A A S G Y P Q P N V T W L Q C S G H T D R C D E A Q V L Q V W D D P Y P E V L S Q E P F H K V T V Q S L L T V E T L E H N Q T Y E C R A H N S V G S G S W A F I P I S A G A H T H P P D E
- Accession
- P07333
- Protein length
- Fragment
- Predicted molecular weight
- 55.1 kDa
- Amino acids
- 20 to 512
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Post-translational modifications
Autophosphorylated in response to CSF1 or IL34 binding (PubMed:20489731, PubMed:23408870, PubMed:24336230). Phosphorylation at Tyr-561 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-561 and Tyr-809 is important for interaction with SRC family members, including FYN, YES1 and SRC, and for subsequent activation of these protein kinases. Phosphorylation at Tyr-699 and Tyr-923 is important for interaction with GRB2. Phosphorylation at Tyr-723 is important for interaction with PIK3R1. Phosphorylation at Tyr-708 is important for normal receptor degradation. Phosphorylation at Tyr-723 and Tyr-809 is important for interaction with PLCG2. Phosphorylation at Tyr-969 is important for interaction with CBL. Dephosphorylation by PTPN2 negatively regulates downstream signaling and macrophage differentiation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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3 product images
SDS-PAGE - Recombinant human CSF-1-R protein (ab155619)
Human CSF-1-R (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 96%.
Functional Studies - Recombinant human CSF-1-R protein (ab155619)
Immobilized ab155619 at 5 μg/mL (100 μL/well) can bind biotinylated human M-CSF, His,Avitag (ab246167) with a linear range of 2-31 ng/mL.
SDS-PAGE - Recombinant human CSF-1-R protein (ab155619)
Purity of ab155619 determined by SDS-PAGE analysis under reduced conditions and staining overnight with coomassie blue.
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