Recombinant Human CSF-1-R protein (Tagged) (Biotin) is a Human Fragment protein, in the 20 to 512 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
Amino acid sequence
I P V I E P S V P E L V V K P G A T V T L R C V G N G S V E W D G P P S P H W T L Y S D G S S S I L S T N N A T F Q N T G T Y R C T E P G D P L G G S A A I H L Y V K D P A R P W N V L A Q E V V V F E D Q D A L L P C L L T D P V L E A G V S L V R V R G R P L M R H T N Y S F S P W H G F T I H R A K F I Q S Q D Y Q C S A L M G G R K V M S I S I R L K V Q K V I P G P P A L T L V P A E L V R I R G E A A Q I V C S A S S V D V N F D V F L Q H N N T K L A I P Q Q S D F H N N R Y Q K V L T L N L D Q V D F Q H A G N Y S C V A S N V Q G K H S T S M F F R V V E S A Y L N L S S E Q N L I Q E V T V G E G L N L K V M V E A Y P G L Q G F N W T Y L G P F S D H Q P E P K L A N A T T K D T Y R H T F T L S L P R L K P S E A G R Y S F L A R N P G G W R A L T F E L T L R Y P P E V S V I W T F I N G S G T L L C A A S G Y P Q P N V T W L Q C S G H T D R C D E A Q V L Q V W D D P Y P E V L S Q E P F H K V T V Q S L L T V E T L E H N Q T Y E C R A H N S V G S G S W A F I P I S A G A H T H P P D E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).
Alternative names
CD115, FMS, CSF1R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R
Recommended products
Recombinant Human CSF-1-R protein (Tagged) (Biotin) is a Human Fragment protein, in the 20 to 512 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
- Accession
- P07333-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- I P V I E P S V P E L V V K P G A T V T L R C V G N G S V E W D G P P S P H W T L Y S D G S S S I L S T N N A T F Q N T G T Y R C T E P G D P L G G S A A I H L Y V K D P A R P W N V L A Q E V V V F E D Q D A L L P C L L T D P V L E A G V S L V R V R G R P L M R H T N Y S F S P W H G F T I H R A K F I Q S Q D Y Q C S A L M G G R K V M S I S I R L K V Q K V I P G P P A L T L V P A E L V R I R G E A A Q I V C S A S S V D V N F D V F L Q H N N T K L A I P Q Q S D F H N N R Y Q K V L T L N L D Q V D F Q H A G N Y S C V A S N V Q G K H S T S M F F R V V E S A Y L N L S S E Q N L I Q E V T V G E G L N L K V M V E A Y P G L Q G F N W T Y L G P F S D H Q P E P K L A N A T T K D T Y R H T F T L S L P R L K P S E A G R Y S F L A R N P G G W R A L T F E L T L R Y P P E V S V I W T F I N G S G T L L C A A S G Y P Q P N V T W L Q C S G H T D R C D E A Q V L Q V W D D P Y P E V L S Q E P F H K V T V Q S L L T V E T L E H N Q T Y E C R A H N S V G S G S W A F I P I S A G A H T H P P D E
- Accession
- P07333
- Protein length
- Fragment
- Predicted molecular weight
- 83 kDa
- Amino acids
- 20 to 512
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, Fc tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Post-translational modifications
Autophosphorylated in response to CSF1 or IL34 binding (PubMed:20489731, PubMed:23408870, PubMed:24336230). Phosphorylation at Tyr-561 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-561 and Tyr-809 is important for interaction with SRC family members, including FYN, YES1 and SRC, and for subsequent activation of these protein kinases. Phosphorylation at Tyr-699 and Tyr-923 is important for interaction with GRB2. Phosphorylation at Tyr-723 is important for interaction with PIK3R1. Phosphorylation at Tyr-708 is important for normal receptor degradation. Phosphorylation at Tyr-723 and Tyr-809 is important for interaction with PLCG2. Phosphorylation at Tyr-969 is important for interaction with CBL. Dephosphorylation by PTPN2 negatively regulates downstream signaling and macrophage differentiation.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Enzymatically biotin-labeled using Avi-tag™ technology.
Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
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SDS-PAGE - Recombinant Human CSF-1-R protein (Tagged) (Biotin) (ab271477)
SDS-PAGE analysis of ab271477.
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