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Recombinant Human CstF-50 protein (denatured) is a Human Full Length protein, in the 1 to 431 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human CstF-50 protein (denatured) (AB130044), expandable thumbnail

Key facts

Purity
>90% SDS-PAGE
Expression system
Escherichia coli
Tags
His tag C-Terminus
Applications
SDS-PAGE
Biologically active
No

Amino acid sequence

M Y R T K V G L K D R Q Q L Y K L I I S Q L L Y D G Y I S I A N G L I N E I K P Q S V C A P S E Q L L H L I K L G M E N D D T A V Q Y A I G R S D T V A P G T G I D L E F D A D V Q T M S P E A S E Y E T C Y V T S H K G P C R V A T Y S R D G Q L I A T G S A D A S I K I L D T E R M L A K S A M P I E V M M N E T A Q Q N M E N H P V I R T L Y D H V D E V T C L A F H P T E Q I L A S G S R D Y T L K L F D Y S K P S A K R A F K Y I Q E A E M L R S I S F H P S G D F I L V G T Q H P T L R L Y D I N T F Q C F V S C N P Q D Q H T D A I C S V N Y N S S A N M Y V T G S K D G C I K L W D G V S N R C I T T F E K A H D G A E V C S A I F S K N S K Y I L S S G K D S V A K L W E I S T G R T L V R Y T G A G L S G R Q V H R T Q A V F N H T E D Y V L L P D E R T I S L C C W D S R T A E R R N L L S L G H N N I V R C I V H S P T N P G F M T C S D D F R A R F W Y R R S T T D V E H H H H H H

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs (PubMed:10669729). May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA (PubMed:10669729).

Alternative names

Recommended products

Recombinant Human CstF-50 protein (denatured) is a Human Full Length protein, in the 1 to 431 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity
>90% SDS-PAGE
Expression system
Escherichia coli
Applications
SDS-PAGE
Accession
Q05048-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 12.01% Urea, 0.58% Sodium chloride, 0.32% Tris HCl

Sequence info

Amino acid sequence

M Y R T K V G L K D R Q Q L Y K L I I S Q L L Y D G Y I S I A N G L I N E I K P Q S V C A P S E Q L L H L I K L G M E N D D T A V Q Y A I G R S D T V A P G T G I D L E F D A D V Q T M S P E A S E Y E T C Y V T S H K G P C R V A T Y S R D G Q L I A T G S A D A S I K I L D T E R M L A K S A M P I E V M M N E T A Q Q N M E N H P V I R T L Y D H V D E V T C L A F H P T E Q I L A S G S R D Y T L K L F D Y S K P S A K R A F K Y I Q E A E M L R S I S F H P S G D F I L V G T Q H P T L R L Y D I N T F Q C F V S C N P Q D Q H T D A I C S V N Y N S S A N M Y V T G S K D G C I K L W D G V S N R C I T T F E K A H D G A E V C S A I F S K N S K Y I L S S G K D S V A K L W E I S T G R T L V R Y T G A G L S G R Q V H R T Q A V F N H T E D Y V L L P D E R T I S L C C W D S R T A E R R N L L S L G H N N I V R C I V H S P T N P G F M T C S D D F R A R F W Y R R S T T D V E H H H H H H
Accession
Q05048
Protein length
Full Length
Predicted molecular weight
49.4 kDa
Amino acids
1 to 431
Nature
Recombinant
Tags
His tag C-Terminus

Specifications

Form
Liquid

General info

Function

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs (PubMed:10669729). May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA (PubMed:10669729).

Post-translational modifications

The N-terminus is blocked.

Subcellular localisation
Nucleus

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human CstF-50 protein (denatured) (ab130044), expandable thumbnail

    SDS-PAGE - Recombinant Human CstF-50 protein (denatured) (ab130044)

    15% SDS-PAGE showing ab130044 (3 µg).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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