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Recombinant human CTLA4 protein (Active) is a Human Fragment protein, in the 37 to 162 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
Alternative names=Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CTLA4, CD152
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, ELISA, FuncS
- Biologically active
Yes
- Sequence
A M H V A Q P A V V L A S S R G I A S F V C E Y A S P G K A T E V R V T V L R Q A D S Q V T E V C A A T Y M M G N E L T F L D D S I C T G T S S G N Q V N L T I Q G L R A M D T G L Y I C K V E L M Y P P P Y Y L G I G N G T Q I Y V I D P E P C P D S D F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Recommended products
Recombinant human CTLA4 protein (Active) is a Human Fragment protein, in the 37 to 162 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
Alternative names=Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CTLA4, CD152
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, ELISA, FuncS
- Biologically active
Yes
- Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (ab173993) at 2μg/mL (100 μL/well) can bind ab167727 with a linear range of 0.16-2.56 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Human B7-2, Fc Tag (ab167720) at 2μg/mL (100 μL/well) can bind ab167727 with a linear range of 1-6.4 ng/mL.
Measured by its binding ability in an SPR assay. Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ab167727 with an affinity constant of 25.7 nM.
- Predicted molecular weight
14.3 kDa
- Amino acids
37 to 162
- Sequence
A M H V A Q P A V V L A S S R G I A S F V C E Y A S P G K A T E V R V T V L R Q A D S Q V T E V C A A T Y M M G N E L T F L D D S I C T G T S S G N Q V N L T I Q G L R A M D T G L Y I C K V E L M Y P P P Y Y L G I G N G T Q I Y V I D P E P C P D S D F
- Accession
- P16410-1
- Protein length
Fragment
- Animal free
No
- Carrier free
Yes
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 99% PBS
Specifications
- Form
Lyophilized
General info
- Function
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
- Post-translational modifications
N-glycosylation is important for dimerization.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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