Recombinant human CTLA4 protein (Active) is a Human Fragment protein, in the 37 to 162 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, ELISA, FuncS
- Biologically active
- Yes
Amino acid sequence
A M H V A Q P A V V L A S S R G I A S F V C E Y A S P G K A T E V R V T V L R Q A D S Q V T E V C A A T Y M M G N E L T F L D D S I C T G T S S G N Q V N L T I Q G L R A M D T G L Y I C K V E L M Y P P P Y Y L G I G N G T Q I Y V I D P E P C P D S D F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Alternative names
CD152, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4
Recommended products
Recombinant human CTLA4 protein (Active) is a Human Fragment protein, in the 37 to 162 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, ELISA, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (ab173993) at 2μg/mL (100 μL/well) can bind ab167727 with a linear range of 0.16-2.56 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Human B7-2, Fc Tag (ab167720) at 2μg/mL (100 μL/well) can bind ab167727 with a linear range of 1-6.4 ng/mL.
Measured by its binding ability in an SPR assay. Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ab167727 with an affinity constant of 25.7 nM.
- Accession
- P16410-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 99% PBS
Sequence info
Amino acid sequence
- A M H V A Q P A V V L A S S R G I A S F V C E Y A S P G K A T E V R V T V L R Q A D S Q V T E V C A A T Y M M G N E L T F L D D S I C T G T S S G N Q V N L T I Q G L R A M D T G L Y I C K V E L M Y P P P Y Y L G I G N G T Q I Y V I D P E P C P D S D F
- Accession
- P16410
- Protein length
- Fragment
- Predicted molecular weight
- 14.3 kDa
- Amino acids
- 37 to 162
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
- Post-translational modifications
N-glycosylation is important for dimerization.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CTLA-4 also known as CD152 is an immune checkpoint receptor with a molecular weight of approximately 34 kDa. This protein is expressed on the surface of T-cells especially after activation and sometimes on regulatory T-cells (Tregs). CTLA-4 competes with CD28 for binding to the same ligands CD80 and CD86 on antigen-presenting cells. Unlike CD28 which stimulates T-cell activation CTLA-4 sends inhibitory signals to downregulate immune responses. By doing this CTLA-4 helps maintain immune system homeostasis and prevents autoimmune reactions.
- Biological function summary
The CTLA-4 protein functions as a critical regulator of the immune system. It interacts with CD80/CD86 in a complex that effectively transmits inhibitory signals to T-cells. CTLA-4 controls the amplitude of the initial activation of T-cells ensuring that the body's immune responses are kept under control. In regulatory T-cells CTLA-4 engagement contributes to their immunosuppressive functions through which they maintain tolerance to self-antigens and prevent overactive immune reactions.
- Pathways
CTLA-4 involves itself in the adaptive immune response pathways specifically the regulation of T-cell activity. CTLA-4's interaction with CD80/CD86 modulates pathways associated with TCR (T-cell receptor) signaling. When CTLA-4 binds its ligands it recruits phosphatases such as SHP-2 that dephosphorylate signaling proteins leading to the downregulation of T-cell interaction. This pathway interaction exemplifies how CTLA-4 acts in concert with proteins like CD28 to finely tune immune responses.
- Associated diseases and disorders
CTLA-4 plays a role in autoimmune diseases and cancer. The dysregulation of CTLA-4 function can lead to autoimmune disorders where the immune system attacks the body's own tissue. In cancer tumor cells may manipulate CTLA-4 pathways to evade immune surveillance. Targeted therapies like anti-CTLA-4 antibodies such as ipilimumab have been developed to block CTLA-4 aiming to enhance the immune system’s ability to attack cancer cells. These treatments highlight the role of CTLA-4 in balancing immune activity and the potential to alter this balance for therapeutic benefit.
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4 product images
SDS-PAGE - Recombinant human CTLA4 protein (Active) (ab167727)
SDS-PAGE of reduced ab167727 stained overnight with Coomassie Blue. The protein migrates as 25-30 kDa under reducing conditions due to glycosylation.
ELISA - Recombinant human CTLA4 protein (Active) (ab167727)
Immobilized Human B7-1, Fc Tag (Recombinant human CD80 protein (Active) ab173993) at 2μg/mL (100 μL/well) can bind ab167727 with a linear range of 0.16-2.56 ng/mL.
ELISA - Recombinant human CTLA4 protein (Active) (ab167727)
Immobilized Human B7-2, Fc Tag (Recombinant human CD86 protein (Fc Chimera) ab167720) at 2μg/mL (100 μL/well) can bind ab167727 with a linear range of 1-6.4 ng/mL.
Functional Studies - Recombinant human CTLA4 protein (Active) (ab167727)
Yervoy (Ipilimumab, Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind ab167727 with an affinity constant of 25.7 nM as determined in SPR assay.
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