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AB132558

Recombinant Human CTP synthase/CTPS protein (GST tag N-Terminus)

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Recombinant Human CTP synthase/CTPS protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 591 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CTPS, CTPS1, CTP synthase 1, CTP synthetase 1, Protein-asparagine deamidase CTPS1, UTP--ammonia ligase 1

1 Images
SDS-PAGE - Recombinant Human CTP synthase/CTPS protein (GST tag N-Terminus) (AB132558)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CTP synthase/CTPS protein (GST tag N-Terminus) (AB132558)

12.5% SDS-PAGE analysis of ab132558 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P17812

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB133743

Anti-CTP synthase/CTPS antibody [EPR8086(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

  • 0
  • 0
Primary Antibodies

AB211498

Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1]

RabMAb|Recombinant

ELISA - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] (AB211498)

  • 0
  • 0
Primary Antibodies

AB106363

Anti-GNPDA2 antibody

Immunocytochemistry - Anti-GNPDA2 antibody (AB106363)

  • 0
  • 0
Primary Antibodies

AB172714

Anti-FAM117A antibody

Western blot - Anti-FAM117A antibody (AB172714)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as CTP synthase.
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Sequence info

[{"sequence":"MKYILVTGGVISGIGKGIIASSVGTILKSCGLHVTSIKIDPYINIDAGTFSPYEHGEVFVLDDGGEVDLDLGNYERFLDIRLTKDNNLTTGKIYQYVINKERKGDYLGKTVQVVPHITDAIQEWVMRQALIPVDEDGLEPQVCVIELGGTVGDIESMPFIEAFRQFQFKVKRENFCNIHVSLVPQPSSTGEQKTKPTQNSVRELRGLGLSPDLVVCRCSNPLDTSVKEKISMFCHVEPEQVICVHDVSSIYRVPLLLEEQGVVDYFLRRLDLPIERQPRKMLMKWKEMADRYDRLLETCSIALVGKYTKFSDSYASVIKALEHSALAINHKLEIKYIDSADLEPITSQEEPVRYHEAWQKLCSAHGVLVPGGFGVRGTEGKIQAIAWARNQKKPFLGVCLGMQLAVVEFSRNVLGWQDANSTEFDPTTSHPVVVDMPEHNPGQMGGTMRLGKRRTLFQTKNSVMRKLYGDADYLEERHRHRFEVNPVWKKCLEEQGLKFVGQDVEGERMEIVELEDHPFFVGVQYHPEFLSRPIKPSPPYFGLLLASVGRLSHYLQKGCRLSPRDTYSDRIGSSSPDSEITELKFPSINHD","proteinLength":"Full Length","predictedMolecularWeight":"90.75 kDa","actualMolecularWeight":null,"aminoAcidEnd":591,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P17812","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTP synthase also known as CTPS or the CTP protein is an enzyme involved in pyrimidine metabolism. It catalyzes the conversion of UTP to CTP an essential step in nucleotide biosynthesis. The enzyme exists in two isoforms CTPS1 and CTPS2 with both found in various tissues but CTPS2 has a higher expression in the brain. CTP synthase has a molecular mass of roughly 70 kDa. CTPS can assemble into filamentous structures under certain conditions influencing its enzymatic activity. These filaments sometimes referred to as cytoophidia are observed in many types of cells.
Biological function summary

CTP synthase plays a significant role in the regulation of nucleotide pools. The enzyme ensures the balance of CTP levels necessary for DNA RNA and phospholipid synthesis acting as a rate-limiting step in these processes. CTPS is part of larger enzymatic complexes that aid in cellular response to nutrient availability and DNA damage. Allosteric regulation and product feedback inhibition modulate CTP synthase activity maintaining cellular homeostasis and a proper supply of CTP for various biosynthetic pathways.

Pathways

CTP synthase is an important component in the pyrimidine biosynthesis pathway essential for nucleic acid and lipid metabolism. The enzyme's activity is tightly linked to the salvage pathways collaborating with enzymes like ribonucleotide reductase to regulate deoxyribonucleotide levels for DNA replication and repair. These interactions highlight the enzyme's involvement in cellular proliferation and response to metabolic stresses underpinning its regulatory function in metabolic pathways.

CTP synthase activity disorders relate to cancer and neurodegeneration. Dysregulation of CTPS is associated with increased proliferation seen in certain cancers given its role in nucleotide biosynthesis. Research also indicates a link between CTPS activity and neurodegenerative diseases as nucleotide imbalance affects neural function. Additionally interactions with proteins involved in cell cycle control and apoptosis such as p53 and Rb further highlight CTPS's role in disease etiology influencing therapeutic strategies targeting these diverse conditions.
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Specifications

Form

Liquid

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General info

Function

CTP synthase involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids (PubMed : 16179339, PubMed : 17189248, PubMed : 17463002, PubMed : 24870241, PubMed : 28459447, PubMed : 34583994). Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen (PubMed : 16179339, PubMed : 24870241, PubMed : 28459447, PubMed : 34583994). CTPS1 CTP synthase activity plays a crucial role in the proliferation of activated lymphocytes and immunity; additional CTP being required to meet increased demand for DNA, RNA and lipid membrane biosynthesis in proliferating lymphocytes (PubMed : 24870241, PubMed : 8530356). In addition to CTP synthase activity, also acts as a protein deamidase that catalyzes the side chain deamidation of specific asparagine residues of proteins to aspartate (PubMed : 40240600). Acts as a negative regulator of innate immunity by mediating deamidation of 'Asn-85' of IRF3, preventing IRF3 from binding DNA (By similarity). Facilitates chromatin relaxation in response to DNA damage by mediating deamidation of 'Asn-76' and 'Asn-77' of histone H1, thereby promoting subsequent acetylation of histone H1 at 'Lys-75' (H1K75ac), increasing chromatin accessibility to facilitate the recruitment of DNA repair proteins (PubMed : 40240600).

Sequence similarities

Belongs to the CTP synthase family.

Post-translational modifications

Phosphorylation at Thr-455 by PKA and PKC inhibits the CTP synthase activity (PubMed:17189248, PubMed:17463002). Phosphorylation at Ser-462 by PKC increases the CTP synthase activity (PubMed:17463002). Phosphorylation by GSK3-beta (GSK3B) inhibits both the CTP synthase and protein deamidase activities (PubMed:17681942). Phosphorylation at Ser-571 by GSK3-beta (GSK3B) constitutes the major phosphorylation site, while phosphorylation at Ser-575 may by required for priming phosphorylation at Ser-571 (PubMed:17681942). Phosphorylation at Ser-575 by GSK3-beta (GSK3B) inhibits its ability to mediate deamination of IRF3 (By similarity).

Subcellular localisation

Nucleus

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Product protocols

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Target data

CTP synthase involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids (PubMed : 16179339, PubMed : 17189248, PubMed : 17463002, PubMed : 24870241, PubMed : 28459447, PubMed : 34583994). Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen (PubMed : 16179339, PubMed : 24870241, PubMed : 28459447, PubMed : 34583994). CTPS1 CTP synthase activity plays a crucial role in the proliferation of activated lymphocytes and immunity; additional CTP being required to meet increased demand for DNA, RNA and lipid membrane biosynthesis in proliferating lymphocytes (PubMed : 24870241, PubMed : 8530356). In addition to CTP synthase activity, also acts as a protein deamidase that catalyzes the side chain deamidation of specific asparagine residues of proteins to aspartate (PubMed : 40240600). Acts as a negative regulator of innate immunity by mediating deamidation of 'Asn-85' of IRF3, preventing IRF3 from binding DNA (By similarity). Facilitates chromatin relaxation in response to DNA damage by mediating deamidation of 'Asn-76' and 'Asn-77' of histone H1, thereby promoting subsequent acetylation of histone H1 at 'Lys-75' (H1K75ac), increasing chromatin accessibility to facilitate the recruitment of DNA repair proteins (PubMed : 40240600).
See full target information CTPS1
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