Recombinant Human CTP synthase/CTPS protein is a Human Full Length protein, in the 1 to 591 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M K Y I L V T G G V I S G I G K G I I A S S V G T I L K S C G L H V T S I K I D P Y I N I D A G T F S P Y E H G E V F V L D D G G E V D L D L G N Y E R F L D I R L T K D N N L T T G K I Y Q Y V I N K E R K G D Y L G K T V Q V V P H I T D A I Q E W V M R Q A L I P V D E D G L E P Q V C V I E L G G T V G D I E S M P F I E A F R Q F Q F K V K R E N F C N I H V S L V P Q P S S T G E Q K T K P T Q N S V R E L R G L G L S P D L V V C R C S N P L D T S V K E K I S M F C H V E P E Q V I C V H D V S S I Y R V P L L L E E Q G V V D Y F L R R L D L P I E R Q P R K M L M K W K E M A D R Y D R L L E T C S I A L V G K Y T K F S D S Y A S V I K A L E H S A L A I N H K L E I K Y I D S A D L E P I T S Q E E P V R Y H E A W Q K L C S A H G V L V P G G F G V R G T E G K I Q A I A W A R N Q K K P F L G V C L G M Q L A V V E F S R N V L G W Q D A N S T E F D P T T S H P V V V D M P E H N P G Q M G G T M R L G K R R T L F Q T K N S V M R K L Y G D A D Y L E E R H R H R F E V N P V W K K C L E E Q G L K F V G Q D V E G E R M E I V E L E D H P F F V G V Q Y H P E F L S R P I K P S P P Y F G L L L A S V G R L S H Y L Q K G C R L S P R D T Y S D R I G S S S P D S E I T E L K F P S I N H D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.
Alternative names
CTPS, CTPS1, CTP synthase 1, CTP synthetase 1, UTP--ammonia ligase 1
Recommended products
Recombinant Human CTP synthase/CTPS protein is a Human Full Length protein, in the 1 to 591 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P17812-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M K Y I L V T G G V I S G I G K G I I A S S V G T I L K S C G L H V T S I K I D P Y I N I D A G T F S P Y E H G E V F V L D D G G E V D L D L G N Y E R F L D I R L T K D N N L T T G K I Y Q Y V I N K E R K G D Y L G K T V Q V V P H I T D A I Q E W V M R Q A L I P V D E D G L E P Q V C V I E L G G T V G D I E S M P F I E A F R Q F Q F K V K R E N F C N I H V S L V P Q P S S T G E Q K T K P T Q N S V R E L R G L G L S P D L V V C R C S N P L D T S V K E K I S M F C H V E P E Q V I C V H D V S S I Y R V P L L L E E Q G V V D Y F L R R L D L P I E R Q P R K M L M K W K E M A D R Y D R L L E T C S I A L V G K Y T K F S D S Y A S V I K A L E H S A L A I N H K L E I K Y I D S A D L E P I T S Q E E P V R Y H E A W Q K L C S A H G V L V P G G F G V R G T E G K I Q A I A W A R N Q K K P F L G V C L G M Q L A V V E F S R N V L G W Q D A N S T E F D P T T S H P V V V D M P E H N P G Q M G G T M R L G K R R T L F Q T K N S V M R K L Y G D A D Y L E E R H R H R F E V N P V W K K C L E E Q G L K F V G Q D V E G E R M E I V E L E D H P F F V G V Q Y H P E F L S R P I K P S P P Y F G L L L A S V G R L S H Y L Q K G C R L S P R D T Y S D R I G S S S P D S E I T E L K F P S I N H D
- Accession
- P17812
- Protein length
- Full Length
- Predicted molecular weight
- 90.75 kDa
- Amino acids
- 1 to 591
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.
- Sequence similarities
Belongs to the CTP synthase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as CTP synthase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CTP synthase also known as CTPS or the CTP protein is an enzyme involved in pyrimidine metabolism. It catalyzes the conversion of UTP to CTP an essential step in nucleotide biosynthesis. The enzyme exists in two isoforms CTPS1 and CTPS2 with both found in various tissues but CTPS2 has a higher expression in the brain. CTP synthase has a molecular mass of roughly 70 kDa. CTPS can assemble into filamentous structures under certain conditions influencing its enzymatic activity. These filaments sometimes referred to as cytoophidia are observed in many types of cells.
- Biological function summary
CTP synthase plays a significant role in the regulation of nucleotide pools. The enzyme ensures the balance of CTP levels necessary for DNA RNA and phospholipid synthesis acting as a rate-limiting step in these processes. CTPS is part of larger enzymatic complexes that aid in cellular response to nutrient availability and DNA damage. Allosteric regulation and product feedback inhibition modulate CTP synthase activity maintaining cellular homeostasis and a proper supply of CTP for various biosynthetic pathways.
- Pathways
CTP synthase is an important component in the pyrimidine biosynthesis pathway essential for nucleic acid and lipid metabolism. The enzyme's activity is tightly linked to the salvage pathways collaborating with enzymes like ribonucleotide reductase to regulate deoxyribonucleotide levels for DNA replication and repair. These interactions highlight the enzyme's involvement in cellular proliferation and response to metabolic stresses underpinning its regulatory function in metabolic pathways.
- Associated diseases and disorders
CTP synthase activity disorders relate to cancer and neurodegeneration. Dysregulation of CTPS is associated with increased proliferation seen in certain cancers given its role in nucleotide biosynthesis. Research also indicates a link between CTPS activity and neurodegenerative diseases as nucleotide imbalance affects neural function. Additionally interactions with proteins involved in cell cycle control and apoptosis such as p53 and Rb further highlight CTPS's role in disease etiology influencing therapeutic strategies targeting these diverse conditions.
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1 product image
SDS-PAGE - Recombinant Human CTP synthase/CTPS protein (ab132558)
12.5% SDS-PAGE analysis of ab132558 stained with Coomassie Blue.
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Product protocols
- Visit the general protocols
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