JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB132558

Recombinant Human CTP synthase/CTPS protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human CTP synthase/CTPS protein is a Human Full Length protein, in the 1 to 591 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CTPS, CTPS1, CTP synthase 1, CTP synthetase 1, UTP--ammonia ligase 1

1 Images
SDS-PAGE - Recombinant Human CTP synthase/CTPS protein (AB132558)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CTP synthase/CTPS protein (AB132558)

12.5% SDS-PAGE analysis of ab132558 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

P17812

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as CTP synthase.
style
left-column

Sequence info

[{"sequence":"MKYILVTGGVISGIGKGIIASSVGTILKSCGLHVTSIKIDPYINIDAGTFSPYEHGEVFVLDDGGEVDLDLGNYERFLDIRLTKDNNLTTGKIYQYVINKERKGDYLGKTVQVVPHITDAIQEWVMRQALIPVDEDGLEPQVCVIELGGTVGDIESMPFIEAFRQFQFKVKRENFCNIHVSLVPQPSSTGEQKTKPTQNSVRELRGLGLSPDLVVCRCSNPLDTSVKEKISMFCHVEPEQVICVHDVSSIYRVPLLLEEQGVVDYFLRRLDLPIERQPRKMLMKWKEMADRYDRLLETCSIALVGKYTKFSDSYASVIKALEHSALAINHKLEIKYIDSADLEPITSQEEPVRYHEAWQKLCSAHGVLVPGGFGVRGTEGKIQAIAWARNQKKPFLGVCLGMQLAVVEFSRNVLGWQDANSTEFDPTTSHPVVVDMPEHNPGQMGGTMRLGKRRTLFQTKNSVMRKLYGDADYLEERHRHRFEVNPVWKKCLEEQGLKFVGQDVEGERMEIVELEDHPFFVGVQYHPEFLSRPIKPSPPYFGLLLASVGRLSHYLQKGCRLSPRDTYSDRIGSSSPDSEITELKFPSINHD","proteinLength":"Full Length","predictedMolecularWeight":"90.75 kDa","actualMolecularWeight":null,"aminoAcidEnd":591,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P17812","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTP synthase also known as CTPS or the CTP protein is an enzyme involved in pyrimidine metabolism. It catalyzes the conversion of UTP to CTP an essential step in nucleotide biosynthesis. The enzyme exists in two isoforms CTPS1 and CTPS2 with both found in various tissues but CTPS2 has a higher expression in the brain. CTP synthase has a molecular mass of roughly 70 kDa. CTPS can assemble into filamentous structures under certain conditions influencing its enzymatic activity. These filaments sometimes referred to as cytoophidia are observed in many types of cells.
Biological function summary

CTP synthase plays a significant role in the regulation of nucleotide pools. The enzyme ensures the balance of CTP levels necessary for DNA RNA and phospholipid synthesis acting as a rate-limiting step in these processes. CTPS is part of larger enzymatic complexes that aid in cellular response to nutrient availability and DNA damage. Allosteric regulation and product feedback inhibition modulate CTP synthase activity maintaining cellular homeostasis and a proper supply of CTP for various biosynthetic pathways.

Pathways

CTP synthase is an important component in the pyrimidine biosynthesis pathway essential for nucleic acid and lipid metabolism. The enzyme's activity is tightly linked to the salvage pathways collaborating with enzymes like ribonucleotide reductase to regulate deoxyribonucleotide levels for DNA replication and repair. These interactions highlight the enzyme's involvement in cellular proliferation and response to metabolic stresses underpinning its regulatory function in metabolic pathways.

CTP synthase activity disorders relate to cancer and neurodegeneration. Dysregulation of CTPS is associated with increased proliferation seen in certain cancers given its role in nucleotide biosynthesis. Research also indicates a link between CTPS activity and neurodegenerative diseases as nucleotide imbalance affects neural function. Additionally interactions with proteins involved in cell cycle control and apoptosis such as p53 and Rb further highlight CTPS's role in disease etiology influencing therapeutic strategies targeting these diverse conditions.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.

Sequence similarities

Belongs to the CTP synthase family.

style
left-column

Product protocols

style
left-column

Target data

This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity.
See full target information CTPS1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB133743

Anti-CTP synthase/CTPS antibody [EPR8086(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CTP synthase/CTPS antibody [EPR8086(B)] (AB133743)

  • 0
  • 0
Primary Antibodies

AB211498

Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1]

RabMAb|Recombinant

ELISA - Anti-8-methyladenosine (m8A) antibody [EPR-20836-125-1] (AB211498)

  • 0
  • 0
Primary Antibodies

AB106363

Anti-GNPDA2 antibody

Immunocytochemistry - Anti-GNPDA2 antibody (AB106363)

  • 0
  • 0
Primary Antibodies

AB172714

Anti-FAM117A antibody

Western blot - Anti-FAM117A antibody (AB172714)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com