Recombinant Human CUL-2 + Elongin-B + Elongin-C + RBX1 + VHL protein (Tagged) is a Human Full Length protein, in the 2 to 108 aa range, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, FuncS.
Images
Publications
Filter by
- The Journal of cell biology 221:2022SARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiying Wang,Chao Liu,Bo Yang,Haotian Zhang,Jian Jiao,Ruidan Zhang,Shujun Liu,Sai Xiao,Yinghong Chen,Bo Liu,Yanjie Ma,Xuefeng Duan,Yueshuai Guo,Mengmeng Guo,Bingbing Wu,Xiangdong Wang,Xingxu Huang,Haitao Yang,Yaoting Gui,Min Fang,Luo Zhang,Shuguang Duo,Xuejiang Guo,Wei LiPubMed 35674692
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
A A A M D V D T P S G T N S G A G K K R F E V K K W N A V A L W A W D I V V D N C A I C R N H I M D L C I E C Q A N Q A S A T S E E C T V A W G V C N H A F H F H C I S R W L K T R Q V C P L D N R E W E F Q K Y G H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins (PubMed:11384984, PubMed:26138980, PubMed:29775578, PubMed:29779948, PubMed:38326650). CUL2 serves as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the E2 ubiquitin-conjugating enzyme (PubMed:10973499, PubMed:11384984, PubMed:12609982, PubMed:24076655, PubMed:9122164, PubMed:38326650). The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (PubMed:12609982, PubMed:24076655, PubMed:27565346, PubMed:38326650). The functional specificity of the ECS complex depends on the substrate recognition component (PubMed:10973499, PubMed:26138980, PubMed:29775578, PubMed:29779948, PubMed:9122164, PubMed:38326650). ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) (PubMed:10973499, PubMed:9122164). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:26138980, PubMed:29775578, PubMed:29779948). ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase (By similarity).
Additional Targets
ELOB, ELOC, RBX1, VHL
Alternative names
Cullin-2, CUL-2, CUL2
Recommended products
Recombinant Human CUL-2 + Elongin-B + Elongin-C + RBX1 + VHL protein (Tagged) is a Human Full Length protein, in the 2 to 108 aa range, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=80% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Assay Conditions: ARV-771-mediated binding of VHL to BET bromodomain was assessed using A-screen technology. Various amounts of VHL were incubated in 10-ul reaction with ARV-771 and GSTBRD3 (BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
- Accession
- Q13617-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- A A A M D V D T P S G T N S G A G K K R F E V K K W N A V A L W A W D I V V D N C A I C R N H I M D L C I E C Q A N Q A S A T S E E C T V A W G V C N H A F H F H C I S R W L K T R Q V C P L D N R E W E F Q K Y G H
- Accession
- P62877
- Protein length
- Full Length
- Predicted molecular weight
- 13 kDa
- Amino acids
- 2 to 108
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 2
- D G E E K T Y G G C E G P D A M Y V K L I S S D G H E F I V K R E H A L T S G T I K A M L S G P G Q F A E N E T N E V N F R E I P S H V L S K V C M Y F T Y K V R Y T N S S T E I P E F P I A P E I A L E L L M A A N F L D C
- Accession
- Q15369
- Protein length
- Fragment
- Predicted molecular weight
- 13 kDa
- Amino acids
- 2 to 112
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 3
- D V F L M I R R H K T T I F T D A K E S S T V F E L K R I V E G I L K R P P D E Q R L Y K D D Q L L D D G K T L G E C G F T S Q T A R P Q A P A T V G L A F R A D D T F E A L C I E P F S S P P E L P D V M K P Q D S G S S A N E Q A V Q
- Accession
- Q15370
- Protein length
- Fragment
- Predicted molecular weight
- 14 kDa
- Amino acids
- 2 to 118
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Amino acid sequence 4
- P R R A E N W D E A E V G A E E A G V E E Y G P E E D G G E E S G A E E S G P E E S G P E E L G A E E E M E A G R P R P V L R S V N S R E P S Q V I F C N R S P R V V L P V W L N F D G E P Q P Y P T L P P G T G R R I H S Y R G H L W L F R D A G T H D G L L V N Q T E L F V P S L N V D G Q P I F A N I T L P V Y T L K E R C L Q V V R S L V K P E N Y R R L D I V R S L Y E D L E D H P N V Q K D L E R L T Q E R I A H Q R M G D
- Accession
- P40337
- Protein length
- Fragment
- Predicted molecular weight
- 25 kDa
- Amino acids
- 2 to 212
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Amino acid sequence 5
- S L K P R V V D F D E T W N K L L T T I K A V V M L E Y V E R A T W N D R F S D I Y A L C V A Y P E P L G E R L Y T E T K I F L E N H V R H L H K R V L E S E E Q V L V M Y H R Y W E E Y S K G A D Y M D C L Y R Y L N T Q F I K K N K L T E A D L Q Y G Y G G V D M N E P L M E I G E L A L D M W R K L M V E P L Q A I L I R M L L R E I K N D R G G E D P N Q K V I H G V I N S F V H V E Q Y K K K F P L K F Y Q E I F E S P F L T E T G E Y Y K Q E A S N L L Q E S N C S Q Y M E K V L G R L K D E E I R C R K Y L H P S S Y T K V I H E C Q Q R M V A D H L Q F L H A E C H N I I R Q E K K N D M A N M Y V L L R A V S T G L P H M I Q E L Q N H I H D E G L R A T S N L T Q E N M P T L F V E S V L E V H G K F V Q L I N T V L N G D Q H F M S A L D K A L T S V V N Y R E P K S V C K A P E L L A K Y C D N L L K K S A K G M T E N E V E D R L T S F I T V F K Y I D D K D V F Q K F Y A R M L A K R L I H G L S M S M D S E E A M I N K L K Q A C G Y E F T S K L H R M Y T D M S V S A D L N N K F N N F I K N Q D T V I D L G I S F Q I Y V L Q A G A W P L T Q A P S S T F A I P Q E L E K S V Q M F E L F Y S Q H F S G R K L T W L H Y L C T G E V K M N Y L G K P Y V A M V T T Y Q M A V L L A F N N S E T V S Y K E L Q D S T Q M N E K E L T K T I K S L L D V K M I N H D S E K E D I D A E S S F S L N M N F S S K R T K F K I T T S M Q K D T P Q E M E Q T R S A V D E D R K M Y L Q A A I V R I M K A R K V L R H N A L I Q E V I S Q S R A R F N P S I S M I K K C I E V L I D K Q Y I E R S Q A S A D E Y S Y V A
- Accession
- Q13617
- Protein length
- Fragment
- Predicted molecular weight
- 88 kDa
- Amino acids
- 2 to 745
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins (PubMed:11384984, PubMed:26138980, PubMed:29775578, PubMed:29779948, PubMed:38326650). CUL2 serves as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the E2 ubiquitin-conjugating enzyme (PubMed:10973499, PubMed:11384984, PubMed:12609982, PubMed:24076655, PubMed:9122164, PubMed:38326650). The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (PubMed:12609982, PubMed:24076655, PubMed:27565346, PubMed:38326650). The functional specificity of the ECS complex depends on the substrate recognition component (PubMed:10973499, PubMed:26138980, PubMed:29775578, PubMed:29779948, PubMed:9122164, PubMed:38326650). ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) (PubMed:10973499, PubMed:9122164). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:26138980, PubMed:29775578, PubMed:29779948). ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase (By similarity).
- Sequence similarities
Belongs to the cullin family.
- Post-translational modifications
Neddylated; which enhances the ubiquitination activity of ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes (PubMed:24076655, PubMed:27565346, PubMed:38326650). Neddylation leads to structural rearrangment in the complex that allows interaction between the E2 ubiquitin-conjugating enzyme and the acceptor ubiquitin (PubMed:38326650). CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Recombinant Human CUL-2 + Elongin-B + Elongin-C + RBX1 + VHL protein (Tagged) (ab271787)
SDS-PAGE analysis ofab271787.
Functional Studies - Recombinant Human CUL-2 + Elongin-B + Elongin-C + RBX1 + VHL protein (Tagged) (ab271787)
Functional studies of ab271787.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com