Recombinant human CXCL14 protein is a Human Full Length protein, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, FuncS, WB.
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Key facts
- Purity
- >98% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
Amino acid sequence
S K C K C S R K G P K I R Y S D V K K L E M K P K Y P H C E E K M V I I T T K S V S R Y R G Q E H C L H P K L Q S T K R F I K W Y N A W N E K R R V Y E E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab50043 can be used as a WB positive control in conjunction with Anti-CXCL14 antibody ab36622. |
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Target data
Function
Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
Alternative names
MIP2G, NJAC, SCYB14, PSEC0212, UNQ240/PRO273, CXCL14, C-X-C motif chemokine 14, Chemokine BRAK, MIP-2G, Small-inducible cytokine B14
Recommended products
Recombinant human CXCL14 protein is a Human Full Length protein, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, FuncS, WB.
Key facts
- Purity
- >98% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
- Biological activity
- Biological Activity : Determined by it's ability to chemoattract activated monocytes using a concentration range of 1.0-10.0 ng/ml.
- Accession
- O95715-1
- Animal free
- No
- Species
- Human
- Concentration
Sequence info
Amino acid sequence
- S K C K C S R K G P K I R Y S D V K K L E M K P K Y P H C E E K M V I I T T K S V S R Y R G Q E H C L H P K L Q S T K R F I K W Y N A W N E K R R V Y E E
- Accession
- O95715
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Greater than 98% by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).
General info
- Function
Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
- Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family.
- Post-translational modifications
Ubiquitinated, followed by degradation by the proteasome.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- A few weeks
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CXCL14 protein also known as BRAK is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of 8.9 kDa and is expressed in various tissues with high levels in the kidney lung skin and reproductive tissues. It does not normally circulate in the blood but rather acts in tissues. The protein plays a role in immune system regulation by being involved in the migration of immune cells.
- Biological function summary
CXCL14 influences cell signaling pathways that regulate immune responses and tissue homeostasis. It engages in the attraction of immune cells like monocytes and dendritic cells to specific tissues. While CXCL14 is not part of a larger complex its function in modulating local immune environments is significant. This protein’s role in immune surveillance contributes to both normal physiological conditions and response to pathology.
- Pathways
Interactions of CXCL14 involve key signaling cascades. The protein is part of pathways that regulate inflammation and cell movement including the MAPK and PI3K signaling pathways. Related proteins such as CXCR4 and CCR2 interact within these pathways to coordinate cell signaling and chemotaxis. These interactions facilitate communication between cells and regulate responses essential for maintaining tissue integrity.
- Associated diseases and disorders
CXCL14 links with conditions like cancer and obesity. Studies suggest that CXCL14 may play a dual role in cancer acting as a tumor suppressor in certain contexts by influencing immune cell infiltration into tumors. In obesity altered expression patterns of CXCL14 have been observed highlighting potential roles in adipose tissue regulation. Proteins like CXCR4 are also connected with CXCL14 in these disease contexts suggesting complex interdependencies within the cellular communication network.
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1 product image
Western blot - Recombinant human CXCL14 protein (ab50043)
All lanes: Western blot - Anti-CXCL14 antibody (Anti-CXCL14 antibody ab36622) at 1 µg/mL
All lanes: Western blot - Recombinant human CXCL14 protein (ab50043) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Exposure time: 12min
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