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Recombinant human CXCL16 protein (His tag) is a Human Fragment protein, in the 1 to 224 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

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Images

Functional Studies - Recombinant human CXCL16 protein (His tag) (AB276323), expandable thumbnail
  • SDS-PAGE - Recombinant human CXCL16 protein (His tag) (AB276323), expandable thumbnail

Key facts

Purity

>95% SDS-PAGE

Endotoxin level
< 1 EU/µg
Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

M G R D L R P G S R V L L L L L L L L L V Y L T Q P G N G N E G S V T G S C Y C G K R I S S D S P P S V Q F M N R L R K H L R A Y H R C L Y Y T R F Q L L S W S V C G G N K D P W V Q E L M S C L D L K E C G H A Y S G I V A H Q K H L L P T S P P I S Q A S E G A S S D I H T P A Q M L L S T L Q S T Q R P T L P V G S L S S D K E L T R P N E T T I H T A G H S L A A G P E A G E N Q K Q P E K N A G P T A R T S A T V P V L C L L A I I F I L T A A L S Y

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info
-
Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Alternative names

Recommended products

Recombinant human CXCL16 protein (His tag) is a Human Fragment protein, in the 1 to 224 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level
< 1 EU/µg
Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS

Biological activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR6.

The ED50 for this effect is 50-250 ng/mL.

Accession
Q9H2A7-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 100% PBS

Sequence info

Amino acid sequence

M G R D L R P G S R V L L L L L L L L L V Y L T Q P G N G N E G S V T G S C Y C G K R I S S D S P P S V Q F M N R L R K H L R A Y H R C L Y Y T R F Q L L S W S V C G G N K D P W V Q E L M S C L D L K E C G H A Y S G I V A H Q K H L L P T S P P I S Q A S E G A S S D I H T P A Q M L L S T L Q S T Q R P T L P V G S L S S D K E L T R P N E T T I H T A G H S L A A G P E A G E N Q K Q P E K N A G P T A R T S A T V P V L C L L A I I F I L T A A L S Y

Accession
Q9H2A7
Protein length

Fragment

Predicted molecular weight

20.5 kDa

Amino acids

1 to 224

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Lyophilized

General info

Function

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Post-translational modifications

Glycosylated.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CXCL16 also known as SR-PSOX is a chemokine that functions primarily in cellular signaling. It weighs approximately 32 kDa and exists in both membrane-bound and soluble forms. Primarily found in the spleen liver lungs and kidneys CXCL16 gets highly expressed on the surface of antigen-presenting cells such as dendritic cells and macrophages. This expression enables CXCL16 to perform its role in cell migration and adhesion by binding to its receptor CXCR6.

Biological function summary

CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.

Pathways

CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.

Associated diseases and disorders

CXCL16 is heavily implicated in atherosclerosis and rheumatoid arthritis. In atherosclerosis CXCL16 contributes to the accumulation of macrophages and foam cell formation in plaques. This connection associates it with inflammatory molecules like TNF-alpha. In rheumatoid arthritis CXCL16 expression is increased in synovial tissues contributing to tissue damage and inflammation potentially linked through interactions with other inflammatory mediators such as IL-6.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Functional Studies - Recombinant human CXCL16 protein (His tag) (ab276323), expandable thumbnail

    Functional Studies - Recombinant human CXCL16 protein (His tag) (ab276323)

    Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR6.

    The ED50 for this effect is 50-250 ng/mL.

  • SDS-PAGE - Recombinant human CXCL16 protein (His tag) (ab276323), expandable thumbnail

    SDS-PAGE - Recombinant human CXCL16 protein (His tag) (ab276323)

    SDS-PAGE analysis of ab276323

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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