Recombinant human CXCL2 protein (Active) is a Human Full Length protein, in the 35 to 107 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
A P L A T E L R C Q C L Q T L Q G I H L K N I Q S V K V K S P G P H C A Q T E V I A T L K N G Q K A C L N P A S P M V K K I I E K M L K N G K S N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
Alternative names
GRO2, GROB, MIP2A, SCYB2, CXCL2, C-X-C motif chemokine 2, Growth-regulated protein beta, Macrophage inflammatory protein 2-alpha, Gro-beta, MIP2-alpha
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Recombinant human CXCL2 protein (Active) is a Human Full Length protein, in the 35 to 107 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- <= 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Human neutrophil chemotaxis (first detectable at 10 ng/mL).
- Accession
- P19875-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.1% Trifluoroacetic acid
Sequence info
Amino acid sequence
- A P L A T E L R C Q C L Q T L Q G I H L K N I Q S V K V K S P G P H C A Q T E V I A T L K N G Q K A C L N P A S P M V K K I I E K M L K N G K S N
- Accession
- P19875
- Protein length
- Full Length
- Predicted molecular weight
- 7.9 kDa
- Amino acids
- 35 to 107
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
- Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family.
- Post-translational modifications
The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CXCL2 protein also known as macrophage inflammatory protein 2 (MIP-2) or KC in the mouse model functions as a chemokine. It weighs approximately 7.8 kDa and is part of the CXC chemokine family. CXCL2 is mainly expressed in macrophages neutrophils and certain epithelial cells. It plays an important role in mediating the migration of these cells to sites of inflammation or injury.
- Biological function summary
CXCL2 attracts and activates neutrophils as part of the innate immune response. It acts independently not as part of a larger complex to provoke a chemotactic response guiding neutrophils to areas of tissue injury. CXCL2 also promotes the release of other cytokines and enzymes that contribute to inflammation. Its activity is mediated through its interaction with receptors like CXCR2 on target cell surfaces.
- Pathways
CXCL2 is involved in inflammatory signaling and leukocyte migration. It holds importance in the chemokine signaling pathway which regulates leukocyte trafficking. CXCL2 interacts with proteins like CXCR2 influencing the inflammation process by activating downstream kinases and other signal transduction molecules such as mitogen-activated protein kinases (MAPKs).
- Associated diseases and disorders
CXCL2 is connected to inflammatory conditions and diseases like sepsis and inflammatory bowel disease (IBD). Elevated levels of CXCL2 can enhance the inflammatory response which is linked to the progression of these conditions. The protein is also associated with tumor necrosis factor-alpha (TNF-alpha) which amplifies inflammation and tissue damage during disease processes.
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1 product image
SDS-PAGE - Recombinant human CXCL2 protein (Active) (ab256023)
SDS-PAGE analysis of ab256023 (1μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions.
4-20% Tris-Glycine gel. Coomassie Blue staining.
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