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AB156728

Recombinant Human CYB5R1 protein (His tag N-Terminus)

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Recombinant Human CYB5R1 protein (His tag N-Terminus) is a Human Fragment protein, in the 29 to 305 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

NQO3A2, UNQ3049/PRO9865, CYB5R1, NADH-cytochrome b5 reductase 1, b5R.1, Humb5R2, NAD(P)H:quinone oxidoreductase type 3 polypeptide A2

1 Images
SDS-PAGE - Recombinant Human CYB5R1 protein (His tag N-Terminus) (AB156728)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human CYB5R1 protein (His tag N-Terminus) (AB156728)

15% SDS-PAGE analysis of ab156728 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9UHQ9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSLVRRSRRPQVTLLDPNEKYLLRLLDKTTVSHNTKRFRFALPTAHHTLGLPVGKHIYLSTRIDGSLVIRPYTPVTSDEDQGYVDLVIKVYLKGVHPKFPEGGKMSQYLDSLKVGDVVEFRGPSGLLTYTGKGHFNIQPNKKSPPEPRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFLLFANQTEKDIILREDLEELQARYPNRFKLWFTLDHPPKDWAYSKGFVTADMIREHLPAPGDDVLVLLCGPPPMVQLACHPNLDKLGYSQKMRFTY","proteinLength":"Fragment","predictedMolecularWeight":"33.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":305,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UHQ9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYB5R1 also known as NADH-cytochrome b5 reductase is an enzyme that plays an important role in electron transfer. It has a molecular mass of approximately 34 kDa and is found in various tissues like liver and erythrocytes. The enzyme exists in different forms within the cell both membrane-bound and soluble which contribute to its diverse functionality. It catalyzes the reduction of cytochrome b5 using NADH as an electron donor acting as a component of the electron transport chain that facilitates different metabolic processes.
Biological function summary

CYB5R1 participates in lipid metabolism and drug metabolism processes. This enzyme works in concert with cytochrome b5 enhancing metabolic activities involving fatty acid desaturation and elongation. It interacts with a wide array of substrates functioning in critical cellular mechanisms including the reduction of methemoglobin to hemoglobin. Although it does not typically form large complexes CYB5R1's activity is essential for maintaining cellular redox balance.

Pathways

CYB5R1 is involved in pathways such as the electron transport chain and fatty acid metabolism. Its activity influences the proper functioning of the electron transfer system by modulating electron transfer to cytochrome b5 which then influences enzymes like fatty acid desaturase. This modulation helps maintain lipid composition within cellular membranes and contributes to energy production in mitochondria.

CYB5R1 has implications in methemoglobinemia and cancer. Mutations or malfunctions in CYB5R1 can lead to an inability to effectively reduce methemoglobin resulting in methemoglobinemia where hemoglobin cannot carry oxygen efficiently. The enzyme's role in drug metabolism also links it to certain cancer types as alterations in its activity can affect drug efficacy and resistance. Its relationships with proteins like cytochrome P450 highlight CYB5R1’s significance in drug metabolism-related disorders.
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Specifications

Form

Liquid

Additional notes

ab156728 is purified using conventional chromatography techniques (anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA).

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General info

Function

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

Sequence similarities

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

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Product protocols

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Target data

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
See full target information CYB5R1
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Complementary Products

Primary Antibodies

AB96103

Anti-CYB5R1 antibody

Western blot - Anti-CYB5R1 antibody (AB96103)

  • 0
  • 0
Primary Antibodies

AB14713

Anti-NDUFA9 antibody [20C11B11B11]

KO Validated

Western blot - Anti-NDUFA9 antibody [20C11B11B11] (AB14713)

  • 4
  • 8
Primary Antibodies

AB110252

Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]

Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (AB110252)

  • 5
  • 1
Primary Antibodies

AB14746

Anti-UQCRFS1/RISP antibody [5A5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [5A5] (AB14746)

  • 4
  • 5

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