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AB180333

Recombinant Human CYB5R2 protein - BSA and Azide free (His tag N-Terminus)

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Recombinant Human CYB5R2 protein - BSA and Azide free (His tag N-Terminus) is a Human Full Length protein, in the 1 to 276 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

NADH-cytochrome b5 reductase 2, b5R.2, CYB5R2

1 Images
SDS-PAGE - Recombinant Human CYB5R2 protein - BSA and Azide free (His tag N-Terminus) (AB180333)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CYB5R2 protein - BSA and Azide free (His tag N-Terminus) (AB180333)

ab180333 at 3 μg analysed by SDS PAGE.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q6BCY4

Animal free

No

Carrier free

Yes

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMNSRRREPITLQDPEAKYPLPLIEKEKISHNTRRFRFGLPSPDHVLGLPVGNYVQLLAKIDNELVVRAYTPVSSDDDRGFVDLIIKIYFKNVHPQYPEGGKMTQYLENMKIGETIFFRGPRGRLFYHGPGNLGIRPDQTSEPKKTLADHLGMIAGGTGITPMLQLIRHITKDPSDRTRMSLIFANQTEEDILVRKELEEIARTHPDQFNLWYTLDRPPIGWKYSSGFVTADMIKEHLPPPAKSTLILVCGPPPLIQTAAHPNLEKLGYTQDMIFTY","proteinLength":"Full Length","predictedMolecularWeight":"33.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":276,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6BCY4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CYB5R2 target also known as cytochrome b5 reductase 2 functions as an enzyme involved in electron transfer processes. It catalyzes the reduction of cytochrome b5 using NADH as an electron donor playing a critical role in the redox reactions. The molecular mass of CYB5R2 is approximately 34 kDa. This protein shows expression in multiple tissues including the liver and testis which suggests its widespread importance in cellular processes.
Biological function summary

Cytochrome b5 reductase 2 facilitates fatty acid desaturation and elongation. This protein does not function alone but often participates as part of larger enzyme complexes. In particular CYB5R2 operates in electron transport processes that are essential for maintaining cellular oxidation-reduction balance. Its activity in these processes highlights its role in maintaining metabolic homeostasis.

Pathways

CYB5R2 engages in fatty acid metabolism and lipid biosynthesis pathways. The protein collaborates with cytochrome P450 enzymes to contribute to lipid modification. These pathways play fundamental roles in energy storage and membrane structure which are necessary for cell function and survival. CYB5R2 shows interactivity with proteins such as CYB5A highlighting the intricate network of cellular electron transport.

Mutations or dysregulation in cytochrome b5 reductase 2 have associations with methemoglobinemia and certain cancers. Methemoglobinemia emerges due to the inability to effectively reduce methemoglobin to hemoglobin causing inefficient oxygen delivery to tissues. The connection with CYB5R2 in this disease involves its critical function in electron transfer. Additionally research indicates links between variations in CYB5R2 and prostate cancer emphasizing its role in cellular growth and proliferation alongside interactions with other proteins such as CYB5A.
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Specifications

Form

Liquid

Additional notes

ab180333 is purified using conventional chromatography techniques.

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General info

Function

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1).

Sequence similarities

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

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Product protocols

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Target data

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1).
See full target information CYB5R2
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