Recombinant Human CYB5R3 protein is a Human Full Length protein, in the 27 to 301 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M F Q R S T P A I T L E S P D I K Y P L R L I D R E I I S H D T R R F R F A L P S P Q H I L G L P V G Q H I Y L S A R I D G N L V V R P Y T P I S S D D D K G F V D L V I K V Y F K D T H P K F P A G G K M S Q Y L E S M Q I G D T I E F R G P S G L L V Y Q G K G K F A I R P D K K S N P I I R T V K S V G M I A G G T G I T P M L Q V I R A I M K D P D D H T V C H L L F A N Q T E K D I L L R P E L E E L R N K H S A R F K L W Y T L D R A P E A W D Y G Q G F V N E E M I R D H L P P P E E E P L V L M C G P P P M I Q Y A C L P N L D H V G H P T E R C F V F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Alternative names
DIA1, CYB5R3, NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1
Recommended products
Recombinant Human CYB5R3 protein is a Human Full Length protein, in the 27 to 301 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P00387-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M F Q R S T P A I T L E S P D I K Y P L R L I D R E I I S H D T R R F R F A L P S P Q H I L G L P V G Q H I Y L S A R I D G N L V V R P Y T P I S S D D D K G F V D L V I K V Y F K D T H P K F P A G G K M S Q Y L E S M Q I G D T I E F R G P S G L L V Y Q G K G K F A I R P D K K S N P I I R T V K S V G M I A G G T G I T P M L Q V I R A I M K D P D D H T V C H L L F A N Q T E K D I L L R P E L E E L R N K H S A R F K L W Y T L D R A P E A W D Y G Q G F V N E E M I R D H L P P P E E E P L V L M C G P P P M I Q Y A C L P N L D H V G H P T E R C F V F
- Accession
- P00387
- Protein length
- Full Length
- Predicted molecular weight
- 34 kDa
- Amino acids
- 27 to 301
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab119468 was purified using conventional chromatography.
General info
- Function
Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
- Sequence similarities
Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
- Subcellular localisation
- Mitochondrion outer membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein CYB5R3 also known as Cytochrome b5 reductase 3 has a molecular mass of approximately 34 kDa. This enzyme is located in various cellular compartments including the outer mitochondrial membrane and the endoplasmic reticulum. CYB5R3 plays a role in electron transfer by reducing cytochrome b5 using NADH or NADPH as electron donors. This reduction is important for various biosynthesis reactions including fatty acid desaturation and elongation as well as steroid biosynthesis.
- Biological function summary
CYB5R3 contributes to the electron transport chain by facilitating the transfer of reducing equivalents to other systems. This enzyme efficiently elevates the electron flow when functioning within a larger enzymatic complex. CYB5R3 is necessary for maintaining redox balance in cells and supports the synthesis of important biomolecules like cholesterol and lipids due to its involvement in these processes.
- Pathways
CYB5R3 integrates into the fatty acid metabolism and steroid biosynthesis pathways. It effectively partners with cytochrome b5 and other reductases to enable efficient electron flux within these pathways. CYB5R3's activity supports metabolic cooperation with other enzymes such as fatty acid desaturase promoting the production and transformation of essential fatty acids.
- Associated diseases and disorders
Mutations or malfunctions in CYB5R3 lead to conditions such as methemoglobinemia and insulin resistance. Methemoglobinemia results from altered oxygen delivery in the blood due to improper reduction of methemoglobin. In this context the reduced function of CYB5R3 affects the ability of related proteins like hemoglobin to bind oxygen efficiently. Insulin resistance involves metabolic alterations where CYB5R3 acts in coordination with proteins associated with lipid metabolism influencing energy homeostasis and insulin action.
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1 product image
SDS-PAGE - Recombinant Human CYB5R3 protein (ab119468)
15% SDS-PAGE analysis of ab119468 (3μg)
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