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AB119468

Recombinant Human CYB5R3 protein (His tag N-Terminus)

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Recombinant Human CYB5R3 protein (His tag N-Terminus) is a Human Full Length protein, in the 27 to 301 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

DIA1, CYB5R3, NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1

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SDS-PAGE - Recombinant Human CYB5R3 protein (His tag N-Terminus) (AB119468)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human CYB5R3 protein (His tag N-Terminus) (AB119468)

15% SDS-PAGE analysis of ab119468 (3μg)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P00387

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF","proteinLength":"Full Length","predictedMolecularWeight":"34 kDa","actualMolecularWeight":null,"aminoAcidEnd":301,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00387","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein CYB5R3 also known as Cytochrome b5 reductase 3 has a molecular mass of approximately 34 kDa. This enzyme is located in various cellular compartments including the outer mitochondrial membrane and the endoplasmic reticulum. CYB5R3 plays a role in electron transfer by reducing cytochrome b5 using NADH or NADPH as electron donors. This reduction is important for various biosynthesis reactions including fatty acid desaturation and elongation as well as steroid biosynthesis.
Biological function summary

CYB5R3 contributes to the electron transport chain by facilitating the transfer of reducing equivalents to other systems. This enzyme efficiently elevates the electron flow when functioning within a larger enzymatic complex. CYB5R3 is necessary for maintaining redox balance in cells and supports the synthesis of important biomolecules like cholesterol and lipids due to its involvement in these processes.

Pathways

CYB5R3 integrates into the fatty acid metabolism and steroid biosynthesis pathways. It effectively partners with cytochrome b5 and other reductases to enable efficient electron flux within these pathways. CYB5R3's activity supports metabolic cooperation with other enzymes such as fatty acid desaturase promoting the production and transformation of essential fatty acids.

Mutations or malfunctions in CYB5R3 lead to conditions such as methemoglobinemia and insulin resistance. Methemoglobinemia results from altered oxygen delivery in the blood due to improper reduction of methemoglobin. In this context the reduced function of CYB5R3 affects the ability of related proteins like hemoglobin to bind oxygen efficiently. Insulin resistance involves metabolic alterations where CYB5R3 acts in coordination with proteins associated with lipid metabolism influencing energy homeostasis and insulin action.
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Specifications

Form

Liquid

Additional notes

ab119468 was purified using conventional chromatography.

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General info

Function

Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.

Sequence similarities

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Subcellular localisation

Mitochondrion outer membrane

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Product protocols

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Target data

Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
See full target information CYB5R3
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Complementary Products

Primary Antibodies

AB133247

Anti-CYB5R3 antibody [EPR3868]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB5R3 antibody [EPR3868] (AB133247)

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