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AB128445

Recombinant Human Cyclin B1 protein (His tag N-Terminus)

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(3 Publications)

Recombinant Human Cyclin B1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 433 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

CCNB, CCNB1, G2/mitotic-specific cyclin-B1

1 Images
SDS-PAGE - Recombinant Human Cyclin B1 protein (His tag N-Terminus) (AB128445)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cyclin B1 protein (His tag N-Terminus) (AB128445)

SDS-PAGE analysis of ab128445 (3 μg) under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P14635

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEEDLCQAFSDVILAVNDVDAEDGADPNLCSEYVKDIYAYLRQLEEEQAVRPKYLLGREVTGNMRAILIDWLVQVQMKFRLLQETMYMTVSIIDRFMQNNCVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTDNTYTKHQIRQMEMKILRALNFGLGRPLPLHFLRRASKIGEVDVEQHTLAKYLMELTMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV","proteinLength":"Full Length","predictedMolecularWeight":"50.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":433,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P14635","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab128445 was purified using conventional chromatography.

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General info

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Sequence similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Post-translational modifications

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132). Deubiquitinated by USP22 during G2/M phase (PubMed:27030811).. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition.
See full target information CCNB1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 146: PubMed30952665

2019

Cyclin B2 is required for progression through meiosis in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Enrico Maria Daldello,Xuan G Luong,Cai-Rong Yang,Jonathan Kuhn,Marco Conti

The Journal of biological chemistry 294:6188-6203 PubMed30782840

2019

Cyclins B1, T1, and H differ in their molecular mode of interaction with cytomegalovirus protein kinase pUL97.

Applications

Unspecified application

Species

Unspecified reactive species

Mirjam Steingruber,Lena Keller,Eileen Socher,Sabrina Ferre,Anne-Marie Hesse,Yohann Couté,Friedrich Hahn,Nicole Büscher,Bodo Plachter,Heinrich Sticht,Manfred Marschall

Viruses 8: PubMed27548200

2016

Proteomic Interaction Patterns between Human Cyclins, the Cyclin-Dependent Kinase Ortholog pUL97 and Additional Cytomegalovirus Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Mirjam Steingruber,Alexandra Kraut,Eileen Socher,Heinrich Sticht,Anna Reichel,Thomas Stamminger,Bushra Amin,Yohann Couté,Corina Hutterer,Manfred Marschall
View all publications
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Complementary Products

Primary Antibodies

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Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 antibody [Y106] (AB32053)

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Proteins & Peptides

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Secondary Antibodies

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Proteins & Peptides

AB126696

Recombinant Human Cyclin A2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Cyclin A2 protein (His tag N-Terminus) (AB126696)

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