Recombinant Human Cyclin D1 protein is a Human Full Length protein, in the 1 to 295 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, WB.
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Publications
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- Nature communications 11:55132020LncRNA DILA1 inhibits Cyclin D1 degradation and contributes to tamoxifen resistance in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianfeng Shi et. al.PubMed 33139730
Key facts
- Purity
- >85% Densitometry
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M E H Q L L C C E V E T I R R A Y P D A N L L N D R V L R A M L K A E E T C A P S V S Y F K C V Q K E V L P S M R K I V A T W M L E V C E E Q K C E E E V F P L A M N Y L D R F L S L E P V K K S R L Q L L G A T C M F V A S K M K E T I P L T A E K L C I Y T D N S I R P E E L L Q M E L L L V N K L K W N L A A M T P H D F I E H F L S K M P E A E E N K Q I I R K H A Q T F V A L C A T D V K F I S N P P S M V A A G S V V A A V Q G L N L R S P N N F L S Y Y R L T R F L S R V I K C D P D C L R A C Q E Q I E A L L E S S L R Q A Q Q N M D P K A A E E E E E E E E E V D L A C T P T D V R D V D I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The protein expressed by the CCND1 gene acts as a regulatory component of the cyclin D1-CDK4 (DC) complex, which phosphorylates and inhibits retinoblastoma (RB) protein family members, including RB1, regulating the cell cycle during the G(1)/S transition. Phosphorylation of RB1 allows the transcription factor E2F to dissociate from the RB/E2F complex, enabling transcription of E2F target genes responsible for G(1) phase progression. In early G(1) phase, it hypophosphorylates RB1. Cyclin D-CDK4 complexes integrate various mitogenic and antimitogenic signals. The protein also serves as a substrate for SMAD3, phosphorylating it in a cell-cycle-dependent manner to repress its transcriptional activity, and forms part of the cyclin D1/CDK4/CDKN1B complex necessary for nuclear translocation and activity. Additionally, it exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters, independent of the cell cycle. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
BCL1, PRAD1, CCND1, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1
Recommended products
Recombinant Human Cyclin D1 protein is a Human Full Length protein, in the 1 to 295 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >85% Densitometry
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P24385-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- M E H Q L L C C E V E T I R R A Y P D A N L L N D R V L R A M L K A E E T C A P S V S Y F K C V Q K E V L P S M R K I V A T W M L E V C E E Q K C E E E V F P L A M N Y L D R F L S L E P V K K S R L Q L L G A T C M F V A S K M K E T I P L T A E K L C I Y T D N S I R P E E L L Q M E L L L V N K L K W N L A A M T P H D F I E H F L S K M P E A E E N K Q I I R K H A Q T F V A L C A T D V K F I S N P P S M V A A G S V V A A V Q G L N L R S P N N F L S Y Y R L T R F L S R V I K C D P D C L R A C Q E Q I E A L L E S S L R Q A Q Q N M D P K A A E E E E E E E E E V D L A C T P T D V R D V D I
- Accession
- P24385
- Protein length
- Full Length
- Amino acids
- 1 to 295
- Nature
- Recombinant
- Tags
- GST tag N-Terminus, His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
The protein expressed by the CCND1 gene acts as a regulatory component of the cyclin D1-CDK4 (DC) complex, which phosphorylates and inhibits retinoblastoma (RB) protein family members, including RB1, regulating the cell cycle during the G(1)/S transition. Phosphorylation of RB1 allows the transcription factor E2F to dissociate from the RB/E2F complex, enabling transcription of E2F target genes responsible for G(1) phase progression. In early G(1) phase, it hypophosphorylates RB1. Cyclin D-CDK4 complexes integrate various mitogenic and antimitogenic signals. The protein also serves as a substrate for SMAD3, phosphorylating it in a cell-cycle-dependent manner to repress its transcriptional activity, and forms part of the cyclin D1/CDK4/CDKN1B complex necessary for nuclear translocation and activity. Additionally, it exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters, independent of the cell cycle.
This supplementary information is collated from multiple sources and compiled automatically.- Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily.
- Post-translational modifications
Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex (PubMed:10766840, PubMed:33854232, PubMed:33854235, PubMed:33854239). It also plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex following DNA damage (PubMed:19412162).
- Subcellular localisation
- Nucleus, Nucleus membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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SDS-PAGE - Recombinant Human Cyclin D1 protein (ab85247)
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