Recombinant Human Cyclin D1 protein is a Human Full Length protein, in the 1 to 295 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, WB.
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Publications
- Nature communications 11:55132020LncRNA DILA1 inhibits Cyclin D1 degradation and contributes to tamoxifen resistance in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianfeng Shi et. al.PubMed 33139730
Key facts
- Purity
>85% Densitometry
- Expression system
Escherichia coli
- Tags
GST tag N-Terminus
His tag N-Terminus- Applications
SDS-PAGE, WB
- Biologically active
No
Amino acid sequence
M E H Q L L C C E V E T I R R A Y P D A N L L N D R V L R A M L K A E E T C A P S V S Y F K C V Q K E V L P S M R K I V A T W M L E V C E E Q K C E E E V F P L A M N Y L D R F L S L E P V K K S R L Q L L G A T C M F V A S K M K E T I P L T A E K L C I Y T D N S I R P E E L L Q M E L L L V N K L K W N L A A M T P H D F I E H F L S K M P E A E E N K Q I I R K H A Q T F V A L C A T D V K F I S N P P S M V A A G S V V A A V Q G L N L R S P N N F L S Y Y R L T R F L S R V I K C D P D C L R A C Q E Q I E A L L E S S L R Q A Q Q N M D P K A A E E E E E E E E E V D L A C T P T D V R D V D I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Alternative names
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1, PRAD1, BCL1, CCND1
Recommended products
Recombinant Human Cyclin D1 protein is a Human Full Length protein, in the 1 to 295 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, WB.
Alternative names
G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1, PRAD1, BCL1, CCND1
Key facts
- Purity
>85% Densitometry
- Expression system
Escherichia coli
- Tags
GST tag N-Terminus
His tag N-Terminus- Applications
SDS-PAGE, WB
- Biologically active
No
- Accession
- P24385-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
M E H Q L L C C E V E T I R R A Y P D A N L L N D R V L R A M L K A E E T C A P S V S Y F K C V Q K E V L P S M R K I V A T W M L E V C E E Q K C E E E V F P L A M N Y L D R F L S L E P V K K S R L Q L L G A T C M F V A S K M K E T I P L T A E K L C I Y T D N S I R P E E L L Q M E L L L V N K L K W N L A A M T P H D F I E H F L S K M P E A E E N K Q I I R K H A Q T F V A L C A T D V K F I S N P P S M V A A G S V V A A V Q G L N L R S P N N F L S Y Y R L T R F L S R V I K C D P D C L R A C Q E Q I E A L L E S S L R Q A Q Q N M D P K A A E E E E E E E E E V D L A C T P T D V R D V D I
- Accession
- Protein length
Full Length
- Amino acids
1 to 295
- Nature
Recombinant
- Tags
GST tag N-Terminus, His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
- Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily.
- Post-translational modifications
Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
- Subcellular localisation
Nucleus, Nucleus membrane
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human Cyclin D1 protein (ab85247)
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