Recombinant human Cyclin D3/CCND3 + Cdk4 protein (Active) is a Human Full Length protein, in the 2 to 292 aa range, expressed in Baculovirus infected Sf9, with >=73% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=73% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
E L L C C E G T R H A P R A G P D P R L L G D Q R V L Q S L L R L E E R Y V P R A S Y F Q C V Q R E I K P H M R K M L A Y W M L E V C E E Q R C E E E V F P L A M N Y L D R Y L S C V P T R K A Q L Q L L G A V C M L L A S K L R E T T P L T I E K L C I Y T D H A V S P R Q L R D W E V L V L G K L K W D L A A V I A H D F L A F I L H R L S L P R D R Q A L V K K H A Q T F L A L C A T D Y T F A M Y P P S M I A T G S I G A A V Q G L G A C S M S G D E L T E L L A G I T G T E V D C L R A C Q E Q I E A A L R E S L R E A S Q T S S S P A P K A P R G S S S Q G P S Q T S T P T D V T A I H L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Additional Targets
G1/S-specific cyclin-D3
Alternative names
Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4
Recommended products
Recombinant human Cyclin D3/CCND3 + Cdk4 protein (Active) is a Human Full Length protein, in the 2 to 292 aa range, expressed in Baculovirus infected Sf9, with >=73% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=73% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity = 10 pmole/min/ug.
Assay was done in a Kinase buffer using Rb (0.02 mg/ml) as a substrate with 10 μM ATP at 30°C for 60 min.
- Accession
- P11802-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.06% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- E L L C C E G T R H A P R A G P D P R L L G D Q R V L Q S L L R L E E R Y V P R A S Y F Q C V Q R E I K P H M R K M L A Y W M L E V C E E Q R C E E E V F P L A M N Y L D R Y L S C V P T R K A Q L Q L L G A V C M L L A S K L R E T T P L T I E K L C I Y T D H A V S P R Q L R D W E V L V L G K L K W D L A A V I A H D F L A F I L H R L S L P R D R Q A L V K K H A Q T F L A L C A T D Y T F A M Y P P S M I A T G S I G A A V Q G L G A C S M S G D E L T E L L A G I T G T E V D C L R A C Q E Q I E A A L R E S L R E A S Q T S S S P A P K A P R G S S S Q G P S Q T S T P T D V T A I H L
- Accession
- P30281
- Protein length
- Full Length
- Amino acids
- 2 to 292
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Amino acid sequence 2
- M A T S R Y E P V A E I G V G A Y G T V Y K A R D P H S G H F V A L K S V R V P N G G G G G G G L P I S T V R E V A L L R R L E A F E H P N V V R L M D V C A T S R T D R E I K V T L V F E H V D Q D L R T Y L D K A P P P G L P A E T I K D L M R Q F L R G L D F L H A N C I V H R D L K P E N I L V T S G G T V K L A D F G L A R I Y S Y Q M A L T P V V V T L W Y R A P E V L L Q S T Y A T P V D M W S V G C I F A E M F R R K P L F C G N S E A D Q L G K I F D L I G L P P E D D W P R D V S L P R G A F P P R G P R P V Q S V V P E M E E S G A Q L L L E M L T F N P H K R I S A F R A L Q H S Y L H K D E G N P E
- Accession
- P11802
- Protein length
- Full Length
- Predicted molecular weight
- 64 kDa
- Amino acids
- 1 to 303
- Nature
- Recombinant
- Tags
- GST tag N-Terminus, His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
- Post-translational modifications
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
- Subcellular localisation
- Nucleus, Nucleus membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Complex of recombinant human CDK4 and Cyclin D3.
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2 product images
Functional Studies - Recombinant human Cyclin D3/CCND3 + Cdk4 protein (Active) (ab271459)
Functional analysis of ab271459.
Specific activity = 10 pmole/min/ug.
Assay was done in a Kinase buffer using Rb (0.02 mg/ml) as a substrate with 10 μM ATP at 30°C for 60 min.
SDS-PAGE - Recombinant human Cyclin D3/CCND3 + Cdk4 protein (Active) (ab271459)
SDS-PAGE analysis of ab271459.
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