Recombinant Hu Cyclin D3/CCND3 + Cdk4 protein (Active) (GST N-Term, GST N-Term + His N-Term)
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Recombinant Hu Cyclin D3/CCND3 + Cdk4 protein (Active) (GST N-Term, GST N-Term + His N-Term) is a Human Full Length protein, in the 2 to 292 aa range, expressed in Baculovirus infected Sf9 cells, with >73%, suitable for SDS-PAGE, FuncS.
View Alternative Names
Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4, G1/S-specific cyclin-D3, CCND3
- FuncS
Unknown
Functional Studies - Recombinant Hu Cyclin D3/CCND3 + Cdk4 protein (Active) (GST N-Term, GST N-Term + His N-Term) (AB271459)
Functional analysis of ab271459.
Specific activity = 10 pmole/min/ug.
Assay was done in a Kinase buffer using Rb (0.02 mg/ml) as a substrate with 10 μM ATP at 30°C for 60 min.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Hu Cyclin D3/CCND3 + Cdk4 protein (Active) (GST N-Term, GST N-Term + His N-Term) (AB271459)
SDS-PAGE analysis of ab271459.
Complementary Products
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Post-translational modifications
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
Subcellular localisation
Nucleus
Target data
Additional targets
Product promise
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