Recombinant human Cyclin E1 + CDK3 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Additional Targets
CDK3
Alternative names
CCNE, CCNE1, G1/S-specific cyclin-E1
Recommended products
Recombinant human Cyclin E1 + CDK3 protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity of ab196131 was determined to be 30 pmol/min/μg. Assay Conditions: Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/mL) substrate and 20 μM ATP at 30°C for 50 min.
- Accession
- P24864-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.64% Sodium chloride, 0.63% Tris HCl, 0.12% Glutathione, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- Accession
- P24864
- Protein length
- Full Length
- Predicted molecular weight
- 73 kDa
- Nature
- Recombinant
Amino acid sequence 2
- Accession
- Q00526
- Protein length
- Full Length
- Predicted molecular weight
- 60 kDa
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Essential for the control of the cell cycle at the G1/S (start) transition.
- Sequence similarities
Belongs to the cyclin family. Cyclin E subfamily.
- Post-translational modifications
Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This is a complex of Cdk3 and Cyclin E1 Co-expressed in a Baculovirus-infected Sf9 cell expression system.
Product promise
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2 product images
Functional Studies - Recombinant human Cyclin E1 + CDK3 protein (Active) (ab196131)
Specific activity of ab196131 was determined to be 30 pmol/min/μg.
SDS-PAGE - Recombinant human Cyclin E1 + CDK3 protein (Active) (ab196131)
10% SDS-PAGE analysis of ab196131 stained with Coomassie Blue.
Lane 1: Cdk3 Lower band and Cyclin E1 upper band
Lane 2: Protein Marker
Downloads
Product protocols
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