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AB119719

Recombinant Human Cyclin E1 protein

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(1 Publication)

Recombinant Human Cyclin E1 protein is a Human Full Length protein, in the 1 to 410 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

CCNE, CCNE1, G1/S-specific cyclin-E1

1 Images
SDS-PAGE - Recombinant Human Cyclin E1 protein (AB119719)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cyclin E1 protein (AB119719)

SDS Page analysis of ab119719

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P24864

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB234617

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB234617)

  • 0
  • 0
Primary Antibodies

AB33911

Anti-Cyclin E1 antibody [EP435E]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin E1 antibody [EP435E] (AB33911)

  • 4
  • 8
Proteins & Peptides

AB111646

Recombinant Human CDK2 protein (His tag C-Terminus)

SDS-PAGE - Recombinant Human CDK2 protein (His tag C-Terminus) (AB111646)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32147

Anti-Cdk2 antibody [E304]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Cdk2 antibody [E304] (AB32147)

  • 5
  • 8
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"73 kDa","actualMolecularWeight":null,"aminoAcidEnd":410,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P24864","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Purity tested by densitometry. Affinity purified.

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General info

Function

Essential for the control of the cell cycle at the G1/S (start) transition.

Sequence similarities

Belongs to the cyclin family. Cyclin E subfamily.

Post-translational modifications

Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

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Product protocols

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Target data

Essential for the control of the cell cycle at the G1/S (start) transition.
See full target information CCNE1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadi3821 PubMed37801505

2023

LncRNA EILA promotes CDK4/6 inhibitor resistance in breast cancer by stabilizing cyclin E1 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Zijie Cai,Qianfeng Shi,Yudong Li,Liang Jin,Shunying Li,Lok Lam Wong,Jingru Wang,Xiaoting Jiang,Mengdi Zhu,Jinna Lin,Qi Wang,Wang Yang,Yujie Liu,Jun Zhang,Chang Gong,Herui Yao,Yandan Yao,Qiang Liu
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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