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AB152261

Recombinant Human Cyclin T1 protein (GST tag N-Terminus)

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Recombinant Human Cyclin T1 protein (GST tag N-Terminus) is a Human Fragment protein, in the 321 to 420 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Cyclin-T1, CycT1, Cyclin-T, CCNT1

1 Images
SDS-PAGE - Recombinant Human Cyclin T1 protein (GST tag N-Terminus) (AB152261)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cyclin T1 protein (GST tag N-Terminus) (AB152261)

12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

O60563

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Primary Antibodies

AB184703

Anti-Cyclin T1 antibody [EPR17982]

RabMAb|Recombinant

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SNLTSVEMLPGKRWLSSQPSFKLEPTQGHRTSENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYAY","proteinLength":"Fragment","predictedMolecularWeight":"36.74 kDa","actualMolecularWeight":null,"aminoAcidEnd":420,"aminoAcidStart":321,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O60563","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II) (PubMed : 16109376, PubMed : 16109377, PubMed : 30134174, PubMed : 35393539). Required to activate the protein kinase activity of CDK9 : acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II (PubMed : 29849146, PubMed : 35393539).. (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.

Sequence similarities

Belongs to the cyclin family. Cyclin C subfamily.

Post-translational modifications

ADP-ribosylation on serine residues by PARP1 in response to DNA damage disrupts the phase separation activity of CCNT1, thereby preventing activation of CDK9.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II) (PubMed : 16109376, PubMed : 16109377, PubMed : 30134174, PubMed : 35393539). Required to activate the protein kinase activity of CDK9 : acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II (PubMed : 29849146, PubMed : 35393539).. (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
See full target information CCNT1
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Product promise

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