Recombinant human Cyclophilin A protein (Active) is a Human Full Length protein, in the 1 to 165 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Images
Publications
Filter by
- JACS Au 1:1147-11572021Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCorinne A Lutomski,Tarick J El-Baba,Jani R Bolla,Carol V RobinsonPubMed 34462738
- Frontiers in immunology 12:6091962021Cyclophilins A, B, and C Role in Human T Lymphocytes Upon Inflammatory Conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra Gegunde,Amparo Alfonso,Rebeca Alvariño,Eva Alonso,Luis M BotanaPubMed 33859635
- Molecular and cellular endocrinology 518:1109902020Extracellular cyclophilin A induces cardiac hypertrophy via the ERK/p47phox pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengfei Cao et. al.PubMed 32805334
- International journal of molecular sciences 21:2019SP-8356, a Novel Inhibitor of CD147-Cyclophilin A Interactions, Reduces Plaque Progression and Stabilizes Vulnerable Plaques in apoE-Deficient Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKisoo Pahk et. al.PubMed 31877775
- Oxidative medicine and cellular longevity 2019:27179862019Cyclophilin A Protects Cardiomyocytes against Hypoxia/Reoxygenation-Induced Apoptosis via the AKT/Nox2 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuyu Cheng et. al.PubMed 31182989
- Frontiers in immunology 7:4522016Secondary Metabolites, Promising Modulators of Immune Response through CD147 Receptor Modulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJon Andoni Sánchez et. al.PubMed 27822214
- Protein and peptide letters 22:672-802015HIV-Enhancing Factors Are Secreted by Reproductive Epithelia upon Inoculation with Bacterial Vaginosis-Associated Bacteria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColleen R Eade,Camila Diaz,Sixue Chen,Amy L Cole,Alexander M ColePubMed 25751267
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M V N P T V F F D I A V D G E P L G R V S F E L F A D K V P K T A E N F R A L S T G E K G F G Y K G S C F H R I I P G F M C Q G G D F T R H N G T G G K S I Y G E K F E D E N F I L K H T G P G I L S M A N A G P N T N G S Q F F I C T A K T E W L D G K H V V F G K V K E G M N I V E A M E R F G S R N G K T S K K I T I A D C G Q L E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed:2001362, PubMed:20676357, PubMed:21245143, PubMed:21593166, PubMed:25678563). Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation (PubMed:11943775, PubMed:21245143). Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1 (PubMed:15130913). Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed:31063815). In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2 (PubMed:23180369). Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1 (PubMed:26095851). Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates (PubMed:25678563). Plays an important role in platelet activation and aggregation (By similarity). Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton (By similarity). Binds heparan sulfate glycosaminoglycans (PubMed:11943775). Inhibits replication of influenza A virus (IAV) (PubMed:19207730). Inhibits ITCH/AIP4-mediated ubiquitination of matrix protein 1 (M1) of IAV by impairing the interaction of ITCH/AIP4 with M1, followed by the suppression of the nuclear export of M1, and finally reduction of the replication of IAV (PubMed:22347431, PubMed:30328013). (Microbial infection) May act as a mediator between human SARS coronavirus nucleoprotein and BSG/CD147 in the process of invasion of host cells by the virus (PubMed:15688292). (Microbial infection) Stimulates RNA-binding ability of HCV NS5A in a peptidyl-prolyl cis-trans isomerase activity-dependent manner.
Alternative names
CYPA, PPIA, Peptidyl-prolyl cis-trans isomerase A, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A
Recommended products
Recombinant human Cyclophilin A protein (Active) is a Human Full Length protein, in the 1 to 165 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is >650 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH8.0 using chymotrypsin. Activity Assay Prepare 180µl assay buffer into a suitable container: Test is 120µl of 200mM Tris-HCl pH8.0, 50µl of 20nM chymotrypsin and 10µl of 100µg/ml Cyclophilin A protein. Blank is 130µl of 200mM Tris-HCl pH8.0 and 50µl of 20nM chymotrypsin. Equilibrate to 37℃ and monitor the A405nm until value is constant using a spectrophotometer. Load 20µl of 5mM Suc-AAPF-pNA into plate. (460mM Lithium chloride, 3mM 2,2,2-Trifluoroethanol in DW. The suc-AAPF-pNA is first dissolved in DMSO and diluted once in 200 mM Tris-HCl (pH8.0). Finally, manufacture the final 5mM with 460mM Lithium chloride, 3mM 2,2,2-Trifluoroethanol). Add 180µl of assay buffer in 5mM Suc-AAPF-pNA. Record the absorbance A405nm for 40 minutes at 37 °C.
- Accession
- P62937-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris, 0.116% Sodium chloride, 0.0077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M V N P T V F F D I A V D G E P L G R V S F E L F A D K V P K T A E N F R A L S T G E K G F G Y K G S C F H R I I P G F M C Q G G D F T R H N G T G G K S I Y G E K F E D E N F I L K H T G P G I L S M A N A G P N T N G S Q F F I C T A K T E W L D G K H V V F G K V K E G M N I V E A M E R F G S R N G K T S K K I T I A D C G Q L E
- Accession
- P62937
- Protein length
- Full Length
- Predicted molecular weight
- 20 kDa
- Actual molecular weight
- 20.18 kDa
- Amino acids
- 1 to 165
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified by using conventional chromatography techniques.
General info
- Function
Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed:2001362, PubMed:20676357, PubMed:21245143, PubMed:21593166, PubMed:25678563). Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation (PubMed:11943775, PubMed:21245143). Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1 (PubMed:15130913). Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed:31063815). In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2 (PubMed:23180369). Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1 (PubMed:26095851). Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates (PubMed:25678563). Plays an important role in platelet activation and aggregation (By similarity). Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton (By similarity). Binds heparan sulfate glycosaminoglycans (PubMed:11943775). Inhibits replication of influenza A virus (IAV) (PubMed:19207730). Inhibits ITCH/AIP4-mediated ubiquitination of matrix protein 1 (M1) of IAV by impairing the interaction of ITCH/AIP4 with M1, followed by the suppression of the nuclear export of M1, and finally reduction of the replication of IAV (PubMed:22347431, PubMed:30328013).
- Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
- Post-translational modifications
Acetylation at Lys-125 markedly inhibits catalysis of cis to trans isomerization and stabilizes cis rather than trans forms of the HIV-1 capsid. PPIA acetylation also antagonizes the immunosuppressive effects of cyclosporine by inhibiting the sequential steps of cyclosporine binding and calcineurin inhibition (PubMed:20364129, Ref.12). Acetylation at Lys-125 favors its interaction with TARDBP (PubMed:25678563).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
MW confirme by MALDI-TOF.
Concentration determined by Bradford assay.
Endotoxin levels determined by LAL method.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant human Cyclophilin A protein (Active) (ab86219)
3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com