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AB78843

Recombinant Human Cyclophilin E protein (His tag N-Terminus)

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Recombinant Human Cyclophilin E protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

CYP33, PPIE, Peptidyl-prolyl cis-trans isomerase E, PPIase E, Cyclophilin E, Cyclophilin-33, Rotamase E

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SDS-PAGE - Recombinant Human Cyclophilin E protein (His tag N-Terminus) (AB78843)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cyclophilin E protein (His tag N-Terminus) (AB78843)

15% SDS-PAGE showing ab78843 at approximately 38kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 210nmol/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH 8.0 using chymotrypsin.

Accession

Q9UNP9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.242% Tris

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDDWLKKFSGKTLEENKEEEGSEPPKAETQEGEPIAKKARSNPQVYMDIKIGNKPAGRIQMLLRSDVVPMTAENFRCLCTHEKGFGFKGSSFHRIIPQFMCQGGDFTNHNGTGGKSIYGKKFDDENFILKHTGPGLLSMANSGPNTNGSQFFLTCDKTDWLDGKHVVFGEVTEGLDVLRQIEAQGSKDGKPKQKVIIADCGEYV","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UNP9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

ab78843 is purified using conventional chromatography techniques.

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General info

Function

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 28076346). Combines RNA-binding and PPIase activities (PubMed : 18258190, PubMed : 20460131, PubMed : 20677832, PubMed : 8977107). Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules (PubMed : 18258190, PubMed : 20460131, PubMed : 8977107). Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins (PubMed : 18258190, PubMed : 20541251, PubMed : 20677832, PubMed : 8977107). Inhibits KMT2A activity; this requires proline isomerase activity (PubMed : 20460131, PubMed : 20541251, PubMed : 20677832).

Sequence similarities

Belongs to the cyclophilin-type PPIase family. PPIase E subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 28076346). Combines RNA-binding and PPIase activities (PubMed : 18258190, PubMed : 20460131, PubMed : 20677832, PubMed : 8977107). Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules (PubMed : 18258190, PubMed : 20460131, PubMed : 8977107). Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins (PubMed : 18258190, PubMed : 20541251, PubMed : 20677832, PubMed : 8977107). Inhibits KMT2A activity; this requires proline isomerase activity (PubMed : 20460131, PubMed : 20541251, PubMed : 20677832).
See full target information PPIE
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Complementary Products

Primary Antibodies

AB249164

Anti-Cyclophilin E antibody [EPR10805(B)] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Cyclophilin E antibody [EPR10805(B)] - BSA and Azide free (AB249164)

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