Recombinant Human Cyclophilin E protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S M A T T K R V L Y V G G L A E E V D D K V L H A A F I P F G D I T D I Q I P L D Y E T E K H R G F A F V E F E L A E D A A A A I D N M N E S E L F G R T I R V N L A K P M R I K E G S S R P V W S D D D W L K K F S G K T L E E N K E E E G S E P P K A E T Q E G E P I A K K A R S N P Q V Y M D I K I G N K P A G R I Q M L L R S D V V P M T A E N F R C L C T H E K G F G F K G S S F H R I I P Q F M C Q G G D F T N H N G T G G K S I Y G K K F D D E N F I L K H T G P G L L S M A N S G P N T N G S Q F F L T C D K T D W L D G K H V V F G E V T E G L D V L R Q I E A Q G S K D G K P K Q K V I I A D C G E Y V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28076346). Combines RNA-binding and PPIase activities (PubMed:18258190, PubMed:20460131, PubMed:20677832, PubMed:8977107). Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules (PubMed:18258190, PubMed:20460131, PubMed:8977107). Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins (PubMed:18258190, PubMed:20541251, PubMed:20677832, PubMed:8977107). Inhibits KMT2A activity; this requires proline isomerase activity (PubMed:20460131, PubMed:20541251, PubMed:20677832).
Alternative names
CYP33, PPIE, Peptidyl-prolyl cis-trans isomerase E, PPIase E, Cyclophilin E, Cyclophilin-33, Rotamase E
Recommended products
Recombinant Human Cyclophilin E protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Specific activity is > 210nmol/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH 8.0 using chymotrypsin.
- Accession
- Q9UNP9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.242% Tris
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S M A T T K R V L Y V G G L A E E V D D K V L H A A F I P F G D I T D I Q I P L D Y E T E K H R G F A F V E F E L A E D A A A A I D N M N E S E L F G R T I R V N L A K P M R I K E G S S R P V W S D D D W L K K F S G K T L E E N K E E E G S E P P K A E T Q E G E P I A K K A R S N P Q V Y M D I K I G N K P A G R I Q M L L R S D V V P M T A E N F R C L C T H E K G F G F K G S S F H R I I P Q F M C Q G G D F T N H N G T G G K S I Y G K K F D D E N F I L K H T G P G L L S M A N S G P N T N G S Q F F L T C D K T D W L D G K H V V F G E V T E G L D V L R Q I E A Q G S K D G K P K Q K V I I A D C G E Y V
- Accession
- Q9UNP9
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab78843 is purified using conventional chromatography techniques.
General info
- Function
Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28076346). Combines RNA-binding and PPIase activities (PubMed:18258190, PubMed:20460131, PubMed:20677832, PubMed:8977107). Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules (PubMed:18258190, PubMed:20460131, PubMed:8977107). Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins (PubMed:18258190, PubMed:20541251, PubMed:20677832, PubMed:8977107). Inhibits KMT2A activity; this requires proline isomerase activity (PubMed:20460131, PubMed:20541251, PubMed:20677832).
- Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase E subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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1 product image
SDS-PAGE - Recombinant Human Cyclophilin E protein (ab78843)
15% SDS-PAGE showing ab78843 at approximately 38kDa (3μg).
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Product protocols
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