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AB132669

Recombinant Human CYP11A1 protein

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(1 Publication)

Recombinant Human CYP11A1 protein is a Human Full Length protein, in the 1 to 521 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CYP11A, CYP11A1, CYPXIA1, Cholesterol desmolase, Cytochrome P450 11A1, Cytochrome P450(scc)

1 Images
SDS-PAGE - Recombinant Human CYP11A1 protein (AB132669)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CYP11A1 protein (AB132669)

12.5% SDS-PAGE analysis of ab132669 stained with Coomassie Blue

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P05108

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MLAKGLPPRSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAAWKKDRVALNQEVMAPEATKNFLPLLDAVSRDFVSVLHRRIKKAGSGNYSGDISDDLFRFAFESITNVIFGERQGMLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDLFRLFRTKTWKDHVAAWDVIFSKADIYTQNFYWELRQKGSVHHDYRGILYRLLGDSKMSFEDIKANVTEMLAGGVDTTSMTLQWHLYEMARNLKVQDMLRAEVLAARHQAQGDMATMLQLVPLLKASIKETLRLHPISVTLQRYLVNDLVLRDYMIPAKTLVQVAIYALGREPTFFFDPENFDPTRWLSKDKNITYFRNLGFGWGVRQCLGRRIAELEMTIFLINMLENFRVEIQHLSDVGTTFNLILMPEKPISFTFWPFNQEATQQ","proteinLength":"Full Length","predictedMolecularWeight":"86.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":521,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P05108","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP11A1 also called cytochrome P450scc is an important enzyme that catalyzes the first step in steroid hormone biosynthesis. This enzyme has a molecular weight of approximately 60 kDa. It is primarily located in the mitochondria of steroidogenic tissues such as the adrenal cortex testes and ovaries. CYP11A1 converts cholesterol into pregnenolone a precursor for all steroid hormones. Its gene expression is tightly regulated by trophic hormones which play an important role in modulating steroid production.
Biological function summary

CYP11A1 is a member of the cytochrome P450 superfamily a large group of enzymes known for their role in oxidation and metabolism. It exists in the mitochondrial electron transport chain working closely with proteins such as ferredoxin and ferredoxin reductase. These interactions facilitate the conversion of cholesterol to pregnenolone. Through these biochemical activities CYP11A1 controls the synthesis rate of steroid hormones influencing various physiological functions including metabolism immune response and reproductive system dynamics.

Pathways

CYP11A1 operates within the steroidogenesis pathway which is essential for the synthesis of steroid hormones like cortisol and aldosterone. It directly interacts with StAR protein which transports cholesterol into mitochondria initiating the steroidogenesis process. Moreover CYP11A1 fits into the pregnenolone biosynthesis pathway showing functional connectivity with other cytochrome P450 enzymes like CYP17A1. These interconnected pathways highlight the role of CYP11A1 as a vital catalyst in hormone synthesis affecting both endocrine and metabolic pathways.

CYP11A1 mutations or expression abnormalities have associations with conditions such as congenital adrenal hyperplasia (CAH) and primary adrenal insufficiency. CAH arises from enzyme deficiencies in the steroidogenesis pathway and changes in CYP11A1 activity can exacerbate the disorder by altering cortisol and aldosterone synthesis. Additionally the enzyme’s link with StAR protein suggests a possible connection to lipoid congenital adrenal hyperplasia where defects in cholesterol transport may disrupt steroid production. Understanding these relationships helps in exploring therapeutic targets for steroid-related diseases.
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Specifications

Form

Liquid

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General info

Function

A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed : 21636783). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed : 21636783). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed : 21636783).

Sequence similarities

Belongs to the cytochrome P450 family.

Subcellular localisation

Mitochondrion inner membrane

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Product protocols

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Target data

A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed : 21636783). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed : 21636783). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed : 21636783).
See full target information CYP11A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:4007-4028 PubMed39113698

2024

promotes gastric cancer through CagA-mediated mitochondrial cholesterol accumulation by targeting CYP11A1 redistribution.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Zhang,Hongxin Huang,Zetian Chen,Mengpei Yan,Chen Lu,Zekuan Xu,Zheng Li
View all publications
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Complementary Products

Primary Antibodies

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Primary Antibodies

AB133657

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Western blot - Anti-StAR antibody [EP7639] (AB133657)

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Proteins & Peptides

AB158428

Recombinant Human Ferredoxin Reductase protein

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Primary Antibodies

AB181067

Anti-VAPA antibody [EPR13589(B)] - N-terminal

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (AB181067)

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