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AB152322

Recombinant Human CYP24A1 protein (GST tag N-Terminus)

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Recombinant Human CYP24A1 protein (GST tag N-Terminus) is a Human Fragment protein, in the 415 to 514 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CYP24, CYP24A1, 24-OHase, Vitamin D(3) 24-hydroxylase, Cytochrome P450 24A1, Cytochrome P450-CC24

1 Images
SDS-PAGE - Recombinant Human CYP24A1 protein (GST tag N-Terminus) (AB152322)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CYP24A1 protein (GST tag N-Terminus) (AB152322)

12.5% SDS-PAGE analysis of ab152322 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

Q07973

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB125001

Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (AB125001)

  • 5
  • 5
Primary Antibodies

AB206655

Anti-CYP27B1 antibody [EPR20271]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27B1 antibody [EPR20271] (AB206655)

  • 4
  • 1
Cell Lines & Lysates

AB273777

Human CYP24A1 knockout A549 cell lysate

Next Generation Sequencing - Human CYP24A1 knockout A549 cell lysate (AB273777)

  • 0
  • 0
Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"LMLNTQVLGSSEDNFEDSSQFRPERWLQEKEKINPFAHLPFGVGKRMCIGRRLAELQLHLALCWIVRKYDIQATDNEPVEMLHSGTLVPSRELPIAFCQR","proteinLength":"Fragment","predictedMolecularWeight":"36.74 kDa","actualMolecularWeight":null,"aminoAcidEnd":514,"aminoAcidStart":415,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q07973","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP24A1 also known as cytochrome P450 family 24 subfamily A member 1 is an enzyme with a molecular mass of approximately 56 kDa. It plays an important role in the catabolism of active vitamin D metabolites particularly in the hydroxylation of calcitriol. This enzyme is located mainly in the kidney but also in other tissues such as the placenta and the pituitary gland. Its expression is regulated by various physiological factors including calcium and phosphate levels.
Biological function summary

This enzyme modulates the levels of active vitamin D influencing calcium and phosphate homeostasis. By converting calcitriol into its inactive forms CYP24A1 prevents excessive vitamin D activity which affects bone mineralization and cellular processes. It does not function as part of a larger complex but interacts closely with other vitamin D-metabolizing enzymes.

Pathways

CYP24A1 is an integral component of the vitamin D metabolism pathway. It acts in concert with another enzyme CYP27B1 which activates vitamin D by hydroxylating calcidiol to calcitriol. Together they balance the hormone levels vital for various cellular functions. The enzyme also participates in calcium signaling pathways affecting cellular growth and differentiation.

Dysregulation of CYP24A1 has been associated with conditions such as hypercalcemia and certain cancers like colorectal cancer. Overexpression or mutations in this enzyme lead to altered vitamin D metabolism exacerbating disease states. In cancer CYP24A1 often interplays with proteins like VDR the vitamin D receptor influencing cancer cell proliferation and response to therapy.
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Specifications

Form

Liquid

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General info

Function

A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) (PubMed : 11012668, PubMed : 15574355, PubMed : 16617161, PubMed : 24893882, PubMed : 29461981, PubMed : 8679605). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed : 15574355, PubMed : 24893882). With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product (PubMed : 11012668, PubMed : 8679605). Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed : 25727742). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed : 8679605).

Sequence similarities

Belongs to the cytochrome P450 family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) (PubMed : 11012668, PubMed : 15574355, PubMed : 16617161, PubMed : 24893882, PubMed : 29461981, PubMed : 8679605). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed : 15574355, PubMed : 24893882). With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product (PubMed : 11012668, PubMed : 8679605). Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol (PubMed : 25727742). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed : 8679605).
See full target information CYP24A1
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