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AB265857

Recombinant human Cystatin A protein (Active) (His tag N-Terminus)

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Recombinant human Cystatin A protein (Active) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 98 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.

View Alternative Names

STF1, STFA, CSTA, Cystatin-A, Cystatin-AS, Stefin-A

2 Images
Functional Studies - Recombinant human Cystatin A protein (Active) (His tag N-Terminus) (AB265857)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Cystatin A protein (Active) (His tag N-Terminus) (AB265857)

Functional analysis of ab265857.

The IC50 value is < 1.0nM. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.

SDS-PAGE - Recombinant human Cystatin A protein (Active) (His tag N-Terminus) (AB265857)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Cystatin A protein (Active) (His tag N-Terminus) (AB265857)

3µg by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE, Mass Spec

applications

Biologically active

Yes

Biological activity

The IC50 value is < 1.0nM. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25°C.

Accession

P01040

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Primary Antibodies

AB128948

Anti-Cystatin A antibody [EPR6941]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Cystatin A antibody [EPR6941] (AB128948)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Secondary Antibodies

AB99697

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP)

VeriBlot

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (AB99697)

  • 5
  • 1
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMIPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVFKSLPGQNEDLVLTGYQVDKNKDDELTGF","proteinLength":"Full Length","predictedMolecularWeight":"13.1 kDa","actualMolecularWeight":"13.1 kDa","aminoAcidEnd":98,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01040","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

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General info

Function

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.

Sequence similarities

Belongs to the cystatin family.

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Product protocols

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Target data

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.
See full target information CSTA
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Product promise

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