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AB283919

Recombinant Human Cystatin C Protein (Active)

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Recombinant Human Cystatin C Protein (Active) is a Human Full Length protein, in the 27 to 146 aa range, expressed in HEK 293 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Cystatin-C, Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, CST3

2 Images
Functional Studies - Recombinant Human Cystatin C Protein (Active) (AB283919)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human Cystatin C Protein (Active) (AB283919)

Fully biologically active determined by its ability to inhibit Papain-induced enzymatic cleavage of the fluorescence peptide substrate Z-FR-AMC. ED50 is ≤ 66.1 ng/ml, corresponding to a specific activity of 1.51 x 105 units/mg.

SDS-PAGE - Recombinant Human Cystatin C Protein (Active) (AB283919)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cystatin C Protein (Active) (AB283919)

SDS-page analysis of ab283919.

Key facts

Purity

>95% SDS-PAGE

Expression system

HEK 293 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by its ability to inhibit Papain-induced enzymatic cleavage of the fluorescence peptide substrate Z-FR-AMC.

ED50 is ≤ 66.1 ng/ml, corresponding to a specific activity of 1.51 x 105 units/mg.

Accession

P01034

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

  • 0
  • 0
Primary Antibodies

AB109508

Anti-Cystatin C antibody [EPR4413]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (AB109508)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB201280

Mouse Cystatin C ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse Cystatin C ELISA Kit (AB201280)

  • 0
  • 0
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Lab Essentials|KO Validated

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"SSPGKPPRLVGGPMDASVEEEGVRRALDFAVGEYNKASNDMYHSRALQVVRARKQIVAGVNYFLDVELGRTTCTKTQPNLDNCPFHDQPHLKRKAFCSFQIYAVPWQGTMTLSKSTCQDA","proteinLength":"Full Length","predictedMolecularWeight":"15.85 kDa","actualMolecularWeight":null,"aminoAcidEnd":146,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P01034","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

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General info

Function

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Sequence similarities

Belongs to the cystatin family.

Post-translational modifications

The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.

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Product protocols

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Target data

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
See full target information CST3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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