Recombinant human Cystatin D protein is a Human Full Length protein, in the 21 to 142 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
G S A S A Q S R T L A G G I H A T D L N D K S V Q C A L D F A I S E Y N K V I N K D E Y Y S R P L Q V M A A Y Q Q I V G G V N Y Y F N V K F G R T T C T K S Q P N L D N C P F N D Q P K L K E E E F C S F Q I N E V P W E D K I S I L N Y K C R K V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B.
Alternative names
Cystatin-D, Cystatin-5, CST5
Recommended products
Recombinant human Cystatin D protein is a Human Full Length protein, in the 21 to 142 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC.
The IC50 value is <15 nM. - Accession
- P28325-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 97% PBS, 3% Trehalose
Sequence info
Amino acid sequence
- G S A S A Q S R T L A G G I H A T D L N D K S V Q C A L D F A I S E Y N K V I N K D E Y Y S R P L Q V M A A Y Q Q I V G G V N Y Y F N V K F G R T T C T K S Q P N L D N C P F N D Q P K L K E E E F C S F Q I N E V P W E D K I S I L N Y K C R K V
- Accession
- P28325
- Protein length
- Full Length
- Predicted molecular weight
- 14.7 kDa
- Amino acids
- 21 to 142
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B.
- Sequence similarities
Belongs to the cystatin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant human Cystatin D protein (ab182715)
The purity of ab182715 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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