Recombinant human Cystatin SA/CST2 protein is a Human Full Length protein, in the 21 to 141 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
W S P Q E E D R I I E G G I Y D A D L N D E R V Q R A L H F V I S E Y N K A T E D E Y Y R R L L R V L R A R E Q I V G G V N Y F F D I E V G R T I C T K S Q P N L D T C A F H E Q P E L Q K K Q L C S F Q I Y E V P W E D R M S L V N S R C Q E A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
Cystatin-SA, Cystatin-2, Cystatin-S5, CST2
Recommended products
Recombinant human Cystatin SA/CST2 protein is a Human Full Length protein, in the 21 to 141 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is < 10.0 nM.
- Accession
- P09228-1
- Animal free
No
- Species
Human
- Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 5% Trehalose, 0.88% Sodium chloride, 0.3% Tris
Sequence info
Amino acid sequence
W S P Q E E D R I I E G G I Y D A D L N D E R V Q R A L H F V I S E Y N K A T E D E Y Y R R L L R V L R A R E Q I V G G V N Y F F D I E V G R T I C T K S Q P N L D T C A F H E Q P E L Q K K Q L C S F Q I Y E V P W E D R M S L V N S R C Q E A
- Accession
- P09228
- Protein length
Full Length
- Predicted molecular weight
15.2 kDa
- Amino acids
21 to 141
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Thiol protease inhibitor.
- Sequence similarities
Belongs to the cystatin family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
Up to 12 months
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cystatin SA also known as CST2 is a member of the cystatin superfamily which are cysteine protease inhibitors. It has a molecular mass of approximately 16 kilodaltons. This protein is expressed mainly in the human saliva and tears where it helps to protect the oral cavity and ocular surface by inhibiting protease activity. The primary role of Cystatin SA is to bind tightly to its target cysteine proteases preventing them from degrading proteins in these sensitive tissues.
- Biological function summary
Cystatin SA plays an important role in maintaining tissue integrity and homeostasis. It is not known to be part of a complex but operates individually to inhibit cysteine proteases like cathepsin B H and L. By controlling the proteolytic activities of these enzymes Cystatin SA helps to prevent excessive tissue breakdown and inflammation which contributes to the preservation of mucosal barriers in the body.
- Pathways
Cystatin SA's inhibitory action plays a significant role in proteolysis and inflammation pathways. One important pathway involves cathepsins which are targeted by Cystatin SA to modulate their activity. Another related pathway includes tissue remodeling where Cystatin SA helps regulate protease activities to maintain structural balance. Both these pathways emphasize its interaction with similar cystatins such as Cystatin C in modulating protease activities to protect tissues.
- Associated diseases and disorders
Cystatin SA has been implicated in conditions such as periodontitis and dry eye syndrome. In periodontitis altered Cystatin SA levels can affect the proteolytic balance contributing to tissue destruction and inflammation. For dry eye syndrome its role in protecting ocular surfaces brings it into focus as a protective factor. Changes in Cystatin SA expression are often correlated with such conditions and it connects with other proteins like cathepsin L which is involved in tissue damage in these disorders.
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2 product images
SDS-PAGE - Recombinant human Cystatin SA/CST2 protein (ab180061)
Human Cystatin SA (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
SDS-PAGE - Recombinant human Cystatin SA/CST2 protein (ab180061)
SDS-PAGE analysis of ab180061 in reducing conditions. Gel stained overnight with Coomassie Blue. DTT-reduced protein migrates as 15-17 kDa.
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