Recombinant Human Cytochrome C Oxidase subunit VIc/COX6C protein is a Human Full Length protein, in the 1 to 75 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A P E V L P K P R M R G L L A R R L R N H M A V A F V L S L G V A A L Y K F R V A D Q R K K A Y A D F Y R N Y D V M K D F E E M R K A G I F Q S V K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Alternative names
Cytochrome c oxidase subunit 6C, Cytochrome c oxidase polypeptide VIc, COX6C
Recommended products
Recombinant Human Cytochrome C Oxidase subunit VIc/COX6C protein is a Human Full Length protein, in the 1 to 75 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P09669-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A P E V L P K P R M R G L L A R R L R N H M A V A F V L S L G V A A L Y K F R V A D Q R K K A Y A D F Y R N Y D V M K D F E E M R K A G I F Q S V K
- Accession
- P09669
- Protein length
- Full Length
- Amino acids
- 1 to 75
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
- Sequence similarities
Belongs to the cytochrome c oxidase subunit 6c family.
- Subcellular localisation
- Mitochondrion inner membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Cytochrome C Oxidase subunit Vic.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytochrome C Oxidase subunit VIc also known as COX6C or cytochrome c oxidase subunit 1 is a component of the enzyme cytochrome c oxidase also referred to as complex IV in the mitochondrial electron transport chain. This subunit contributes significantly to the mechanical activity by aiding the catalytic conversion of molecular oxygen to water. COX6C is relatively lightweight compared to other subunits with an approximate mass of 7.5 kDa. The expression of COX6C predominantly occurs in tissues with high metabolic activity such as the heart muscle and brain where energetic demands are substantial.
- Biological function summary
Cytochrome C Oxidase subunit VIc is part of the larger cytochrome c oxidase complex facilitating efficient electron transfer from cytochrome c to oxygen therefore promoting the process of oxidative phosphorylation. This activity is critical for sustaining the mitochondrial membrane potential and ATP production. COX6C's integration within the complex is necessary for its structural integrity and optimal performance. This subunit impacts mitochondrial functionality playing a pivotal role in cellular energy metabolism.
- Pathways
Cytochrome C Oxidase subunit VIc contributes to the oxidative phosphorylation pathway and impacts the electron transport chain's efficiency. Its function within this pathway is interconnected with cytochrome c and other cytochrome oxidase complexes. During this process ATP synthase takes advantage of the proton gradient established by the electron flow managed by complex IV. COX6C's role within these pathways ensures effective energy conversion maintaining cellular respiration and energy output in aerobically respiring cells.
- Associated diseases and disorders
Abnormalities in cytochrome C Oxidase subunit VIc expression or function often lead to mitochondrial diseases such as Leigh syndrome and cytochrome c oxidase deficiency. These conditions are linked with defects in cellular respiration causing a wide array of symptoms including neurological and muscular impairments. The subunit's relationship with cytochrome c integral to both electron transfer and apoptosis pathways highlights its influence in these mitochondrial disorders. Understanding COX6C's role offers insights into potential therapeutic targets to mitigate these conditions.
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1 product image
SDS-PAGE - Recombinant Human Cytochrome C Oxidase subunit VIc/COX6C protein (ab158177)
ab158177 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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