Recombinant Human Cytochrome P450 17A1/CYP17A1 protein is a Human Full Length protein, in the 1 to 508 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M W E L V A L L L L T L A Y L F W P K R R C P G A K Y P K S L L S L P L V G S L P F L P R H G H M H N N F F K L Q K K Y G P I Y S V R M G T K T T V I V G H H Q L A K E V L I K K G K D F S G R P Q M A T L D I A S N N R K G I A F A D S G A H W Q L H R R L A M A T F A L F K D G D Q K L E K I I C Q E I S T L C D M L A T H N G Q S I D I S F P V F V A V T N V I S L I C F N T S Y K N G D P E L N V I Q N Y N E G I I D N L S K D S L V D L V P W L K I F P N K T L E K L K S H V K I R N D L L N K I L E N Y K E K F R S D S I T N M L D T L M Q A K M N S D N G N A G P D Q D S E L L S D N H I L T T I G D I F G A G V E T T T S V V K W T L A F L L H N P Q V K K K L Y E E I D Q N V G F S R T P T I S D R N R L L L L E A T I R E V L R L R P V A P M L I P H K A N V D S S I G E F A V D K G T E V I I N L W A L H H N E K E W H Q P D Q F M P E R F L N P A G T Q L I S P S V S Y L P F G A G P R S C I G E I L A R Q E L F L I M A W L L Q R F D L E V P D D G Q L P S L E G I P K V V F L I D S F K V K I K V R Q A W R E A Q A E G S T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis (PubMed:22266943, PubMed:25301938, PubMed:27339894, PubMed:9452426). Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (Probable) (PubMed:25301938, PubMed:9452426). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione (PubMed:22266943, PubMed:25301938, PubMed:27339894, PubMed:36640554, PubMed:9452426). Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA (PubMed:36640554). Also 16-alpha hydroxylates androgens, relevant for estriol synthesis (PubMed:25301938, PubMed:27339894). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:22266943, PubMed:25301938, PubMed:27339894, PubMed:9452426).
Alternative names
CYP17, S17AH, CYP17A1, 17-alpha-hydroxyprogesterone aldolase, CYPXVII, Cytochrome P450 17A1, Cytochrome P450-C17, Steroid 17-alpha-monooxygenase, Cytochrome P450c17
Recommended products
Recombinant Human Cytochrome P450 17A1/CYP17A1 protein is a Human Full Length protein, in the 1 to 508 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P05093-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M W E L V A L L L L T L A Y L F W P K R R C P G A K Y P K S L L S L P L V G S L P F L P R H G H M H N N F F K L Q K K Y G P I Y S V R M G T K T T V I V G H H Q L A K E V L I K K G K D F S G R P Q M A T L D I A S N N R K G I A F A D S G A H W Q L H R R L A M A T F A L F K D G D Q K L E K I I C Q E I S T L C D M L A T H N G Q S I D I S F P V F V A V T N V I S L I C F N T S Y K N G D P E L N V I Q N Y N E G I I D N L S K D S L V D L V P W L K I F P N K T L E K L K S H V K I R N D L L N K I L E N Y K E K F R S D S I T N M L D T L M Q A K M N S D N G N A G P D Q D S E L L S D N H I L T T I G D I F G A G V E T T T S V V K W T L A F L L H N P Q V K K K L Y E E I D Q N V G F S R T P T I S D R N R L L L L E A T I R E V L R L R P V A P M L I P H K A N V D S S I G E F A V D K G T E V I I N L W A L H H N E K E W H Q P D Q F M P E R F L N P A G T Q L I S P S V S Y L P F G A G P R S C I G E I L A R Q E L F L I M A W L L Q R F D L E V P D D G Q L P S L E G I P K V V F L I D S F K V K I K V R Q A W R E A Q A E G S T
- Accession
- P05093
- Protein length
- Full Length
- Predicted molecular weight
- 81.62 kDa
- Amino acids
- 1 to 508
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis (PubMed:22266943, PubMed:25301938, PubMed:27339894, PubMed:9452426). Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (Probable) (PubMed:25301938, PubMed:9452426). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione (PubMed:22266943, PubMed:25301938, PubMed:27339894, PubMed:36640554, PubMed:9452426). Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA (PubMed:36640554). Also 16-alpha hydroxylates androgens, relevant for estriol synthesis (PubMed:25301938, PubMed:27339894). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:22266943, PubMed:25301938, PubMed:27339894, PubMed:9452426).
- Sequence similarities
Belongs to the cytochrome P450 family.
- Post-translational modifications
Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Cytochrome P450 17A1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytochrome P450 17A1 also known as CYP17A1 is an essential enzyme in the steroidogenic pathway. Its molecular mass is approximately 57 kDa. It is part of the cytochrome P450 family and shows expression primarily in the adrenal glands and gonads. This enzyme catalyzes two key reactions: the 17α-hydroxylation of pregnenolone and progesterone and the subsequent 1720-lyase reaction. These reactions are critical for the production of steroid hormones such as glucocorticoids and sex steroids.
- Biological function summary
CYP17A1 plays a central role in hormone biosynthesis. The enzyme exists as a single protein and does not form part of a larger complex. By facilitating the conversion of progestogens into androgens it directly impacts the balance and levels of steroid hormones in the body. This function supports vital processes like the development of secondary sexual characteristics and the regulation of metabolism therefore affecting multiple physiological functions.
- Pathways
CYP17A1 integrates into both the steroidogenesis and biosynthesis pathways of adrenal and sex hormones. It links directly with proteins such as steroidogenic acute regulatory protein (StAR) and cytochrome P450 oxidoreductase (POR) which assist in enabling its enzymatic function. These pathways regulate the levels of hormones influencing biological processes including stress responses and reproductive functions.
- Associated diseases and disorders
CYP17A1 is associated with conditions like congenital adrenal hyperplasia and prostate cancer. In congenital adrenal hyperplasia CYP17A1 mutations disrupt the steroidogenesis pathway leading to cortisol production deficiency. In prostate cancer CYP17A1 is often upregulated contributing to increased androgen levels that fuel cancer progression. Proteins such as 3β-hydroxysteroid dehydrogenase (3β-HSD) and androgen receptor (AR) interact with CYP17A1 in these disorders demonstrating its importance in disease mechanisms.
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1 product image
SDS-PAGE - Recombinant Human Cytochrome P450 17A1/CYP17A1 protein (ab152320)
12.5% SDS-PAGE analysis of ab152320 stained with Coomassie Blue.
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